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The map label for this gene is azoR1 [H]

Identifier: 50364861

GI number: 50364861

Start: 61953

End: 62552

Strand: Reverse

Name: azoR1 [H]

Synonym: Mfl046

Alternate gene names: 50364861

Gene position: 62552-61953 (Counterclockwise)

Preceding gene: 50364865

Following gene: 50364850

Centisome position: 7.89

GC content: 29.83

Gene sequence:

>600_bases
ATGAGTAAATTATTAGTTATAAATGGTTCTGTTATTCCAAGTGATAAATCAAATTCACACGAAATGGCAAGAATATTTTT
AGAGGAATACAAAAAAGTTAATCCAAATGACGAGATTATTGAATTAGATTTAAATAAACTTGTTGTCGGAACAAATGTTT
TAACAACTGAAACTTTTTCAACTTATTGAGGTGAAGAAGAAGGAATGAAATATATTAATCAATTAAAAGATGTTGATAAA
TTATTAGTTATCGCTCCAATGTATAACTTCCATGTTTCTGGAATGTTAAAAAACTATATTGACCATGTTGCTTTAGCTAA
CCAAACTTTTTCTTATAAATACGCAACAAAGGGTGCAAGCATTGGTTTATTAGATAAACTTAAAGTTCAAATACTTGCTA
CTCAAGGAGCTCCAAAAGGTTGATACCCATGAGGAGATCATGTAGCATATTTAAAAGGTACATGAGAATTTATGGGGGCA
AAAGTTGCTGAACCTATTTTATTAGCTGGTGTTAAGGTTGAACCACTAAGTACTCAATCTCCAAAAGAAATTGTTTCATC
AATAACTTCTGATTTGATTGCTGCTGCTAAGAAATTTTAA

Upstream 100 bases:

>100_bases
TTTTCTTTTAACATTTTTATATTCCTTTTCAGTTGTTTTGTTAATATTGATTATAAACTTTTAGTGATATAATTAAAATA
ATAAAAAGGAGAATATTTTT

Downstream 100 bases:

>100_bases
ATTAAAATCGGGTTTCCCCGATTTTTTTATTTTGTTGCTTTTTTAGTTGCTGCTGTCAGTGCTTCTTGAATTATTTTTTC
AAAGTTTTTATTATCTAAAA

Product: azoreductase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MSKLLVINGSVIPSDKSNSHEMARIFLEEYKKVNPNDEIIELDLNKLVVGTNVLTTETFSTYWGEEEGMKYINQLKDVDK
LLVIAPMYNFHVSGMLKNYIDHVALANQTFSYKYATKGASIGLLDKLKVQILATQGAPKGWYPWGDHVAYLKGTWEFMGA
KVAEPILLAGVKVEPLSTQSPKEIVSSITSDLIAAAKKF

Sequences:

>Translated_199_residues
MSKLLVINGSVIPSDKSNSHEMARIFLEEYKKVNPNDEIIELDLNKLVVGTNVLTTETFSTY*GEEEGMKYINQLKDVDK
LLVIAPMYNFHVSGMLKNYIDHVALANQTFSYKYATKGASIGLLDKLKVQILATQGAPKG*YP*GDHVAYLKGT*EFMGA
KVAEPILLAGVKVEPLSTQSPKEIVSSITSDLIAAAKKF
>Mature_198_residues
SKLLVINGSVIPSDKSNSHEMARIFLEEYKKVNPNDEIIELDLNKLVVGTNVLTTETFSTY*GEEEGMKYINQLKDVDKL
LVIAPMYNFHVSGMLKNYIDHVALANQTFSYKYATKGASIGLLDKLKVQILATQGAPKG*YP*GDHVAYLKGT*EFMGAK
VAEPILLAGVKVEPLSTQSPKEIVSSITSDLIAAAKKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=146, Percent_Identity=32.1917808219178, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 3.1.4.14

Molecular weight: Translated: 21312; Mature: 21181

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLLVINGSVIPSDKSNSHEMARIFLEEYKKVNPNDEIIELDLNKLVVGTNVLTTETFS
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEEEECHHH
TYGEEEGMKYINQLKDVDKLLVIAPMYNFHVSGMLKNYIDHVALANQTFSYKYATKGASI
HCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GLLDKLKVQILATQGAPKGYPGDHVAYLKGTEFMGAKVAEPILLAGVKVEPLSTQSPKEI
CCHHHEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHCCHHEEECCEECCCCCCCHHHH
VSSITSDLIAAAKKF
HHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKLLVINGSVIPSDKSNSHEMARIFLEEYKKVNPNDEIIELDLNKLVVGTNVLTTETFS
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCEEEECCCEEEECHHH
TYGEEEGMKYINQLKDVDKLLVIAPMYNFHVSGMLKNYIDHVALANQTFSYKYATKGASI
HCCHHHHHHHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GLLDKLKVQILATQGAPKGYPGDHVAYLKGTEFMGAKVAEPILLAGVKVEPLSTQSPKEI
CCHHHEEEEEEEECCCCCCCCCCEEEEEECCCCCCHHHCCHHEEECCEECCCCCCCHHHH
VSSITSDLIAAAKKF
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA