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The map label for this gene is ybfF [H]

Identifier: 50085219

GI number: 50085219

Start: 2085041

End: 2085841

Strand: Reverse

Name: ybfF [H]

Synonym: ACIAD2099

Alternate gene names: 50085219

Gene position: 2085841-2085041 (Counterclockwise)

Preceding gene: 50085221

Following gene: 50085215

Centisome position: 57.96

GC content: 37.33

Gene sequence:

>801_bases
ATGGAAAACTTGATGATATTACATAGTCACATTGTTGAATCGGATCACGCAACAACTAACAAGCCAGTGGTCTTATTGCA
CGGTTTATTGGGTAGTTTATCTAATTTGGGCGTTATTGCACGGGCTCTAAATACAAATCATAAAATTATACAGCTCGATT
TACGTAATCACGGTTTATCGTCACATAGTGATGAAATGAACTATGAAATTATGGCACAAGATGTAATTGATACCCTAGAT
GAACTCGGTATTGAGCAATTTTCTTTGATTGGACACTCGATGGGCGGAAAGGTTTGCATGAAAATTGCAGGTCTTTATCC
GAATCGTGTGGATAAATTAATTGTATTGGATATTAGTCCAGTGGTTTATCAGGGACATCGACATCAGGACATTTTAAAAG
CCATCAATGCTGTGCGCGCTGAACCTCATGAACTGAGTCGCAAGCAAGCCACTGAAATTATGAAGCAGTTTATTCCACAA
GATGGCATCATTCTCTTCTTGCTTAAATCTTTTAATCAAGGGCATTGGCTATTTAATGTCGACGCAATTGAAAAACAGTA
TCCAAGTATTACAGGATGGAAGGATATTCAACCGTGGTTGAAACCATGTCTATTTATTAAAGGAGCAAATTCTGATTATA
TCGAAATGGAATATGAAAATAATATTCGTCAGCAGTTTCCATCAGCCGAAATTAAATCAATCGAAGGTGTAGGGCATTGG
TTACATGCTGAAAAACCTCAGGAAGTGATCAAGTTGATTCAGCAATTTTTGCAAGAACAGGGCGAATCACGTGATTATTA
A

Upstream 100 bases:

>100_bases
AAAATCTGTTGAGAGCAGTTTTTTATTTGATGATGAAATGTAGTTGCTGCTTGAATCATTCTATCTCGTCGCGCTAATCT
ATTGATAGAGCAATAAATAG

Downstream 100 bases:

>100_bases
TTACTCAATATGAGCAAGGACAGCACATTTTCTATGTGAGTATTGTCCAAAGCAGCAACCAGTGTTTTGGACAGATAGAT
TTATTTGATTTAATCAGGAT

Product: alpha/beta family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MENLMILHSHIVESDHATTNKPVVLLHGLLGSLSNLGVIARALNTNHKIIQLDLRNHGLSSHSDEMNYEIMAQDVIDTLD
ELGIEQFSLIGHSMGGKVCMKIAGLYPNRVDKLIVLDISPVVYQGHRHQDILKAINAVRAEPHELSRKQATEIMKQFIPQ
DGIILFLLKSFNQGHWLFNVDAIEKQYPSITGWKDIQPWLKPCLFIKGANSDYIEMEYENNIRQQFPSAEIKSIEGVGHW
LHAEKPQEVIKLIQQFLQEQGESRDY

Sequences:

>Translated_266_residues
MENLMILHSHIVESDHATTNKPVVLLHGLLGSLSNLGVIARALNTNHKIIQLDLRNHGLSSHSDEMNYEIMAQDVIDTLD
ELGIEQFSLIGHSMGGKVCMKIAGLYPNRVDKLIVLDISPVVYQGHRHQDILKAINAVRAEPHELSRKQATEIMKQFIPQ
DGIILFLLKSFNQGHWLFNVDAIEKQYPSITGWKDIQPWLKPCLFIKGANSDYIEMEYENNIRQQFPSAEIKSIEGVGHW
LHAEKPQEVIKLIQQFLQEQGESRDY
>Mature_266_residues
MENLMILHSHIVESDHATTNKPVVLLHGLLGSLSNLGVIARALNTNHKIIQLDLRNHGLSSHSDEMNYEIMAQDVIDTLD
ELGIEQFSLIGHSMGGKVCMKIAGLYPNRVDKLIVLDISPVVYQGHRHQDILKAINAVRAEPHELSRKQATEIMKQFIPQ
DGIILFLLKSFNQGHWLFNVDAIEKQYPSITGWKDIQPWLKPCLFIKGANSDYIEMEYENNIRQQFPSAEIKSIEGVGHW
LHAEKPQEVIKLIQQFLQEQGESRDY

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200008, Length=246, Percent_Identity=36.9918699186992, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI23200012, Length=246, Percent_Identity=36.9918699186992, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI223941839, Length=238, Percent_Identity=31.5126050420168, Blast_Score=121, Evalue=6e-28,
Organism=Escherichia coli, GI1786902, Length=241, Percent_Identity=50.207468879668, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17508535, Length=255, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17507063, Length=261, Percent_Identity=28.3524904214559, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321468, Length=285, Percent_Identity=30.5263157894737, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24640348, Length=260, Percent_Identity=30, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24646055, Length=258, Percent_Identity=23.2558139534884, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 30317; Mature: 30317

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLMILHSHIVESDHATTNKPVVLLHGLLGSLSNLGVIARALNTNHKIIQLDLRNHGLS
CCCEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
SHSDEMNYEIMAQDVIDTLDELGIEQFSLIGHSMGGKVCMKIAGLYPNRVDKLIVLDISP
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCH
VVYQGHRHQDILKAINAVRAEPHELSRKQATEIMKQFIPQDGIILFLLKSFNQGHWLFNV
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEHEEHHCCCCCEEEEE
DAIEKQYPSITGWKDIQPWLKPCLFIKGANSDYIEMEYENNIRQQFPSAEIKSIEGVGHW
HHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCEEEEEHHCCHHHHCCCHHHHHHHHHHHH
LHAEKPQEVIKLIQQFLQEQGESRDY
HCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MENLMILHSHIVESDHATTNKPVVLLHGLLGSLSNLGVIARALNTNHKIIQLDLRNHGLS
CCCEEEHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCC
SHSDEMNYEIMAQDVIDTLDELGIEQFSLIGHSMGGKVCMKIAGLYPNRVDKLIVLDISP
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCH
VVYQGHRHQDILKAINAVRAEPHELSRKQATEIMKQFIPQDGIILFLLKSFNQGHWLFNV
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEHEEHHCCCCCEEEEE
DAIEKQYPSITGWKDIQPWLKPCLFIKGANSDYIEMEYENNIRQQFPSAEIKSIEGVGHW
HHHHHHCCCCCCCCHHHHHHHHEEEEECCCCCEEEEEHHCCHHHHCCCHHHHHHHHHHHH
LHAEKPQEVIKLIQQFLQEQGESRDY
HCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]