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The map label for this gene is ispF

Identifier: 50085131

GI number: 50085131

Start: 1987409

End: 1987900

Strand: Reverse

Name: ispF

Synonym: ACIAD1996

Alternate gene names: 50085131

Gene position: 1987900-1987409 (Counterclockwise)

Preceding gene: 161349963

Following gene: 50085124

Centisome position: 55.24

GC content: 41.06

Gene sequence:

>492_bases
GTGGTTGTACAAATTAGAATTGGTCAGGGAATGGATGTTCATGCTTTTGAAGAGGGTGATCATGTTACTTTGGCTGGCAT
AAAAATTCCTCATACTCATGGTTTAAAAGCGCATTCAGATGGAGATGTAGTGTTACACGCATTGTCGGATGCATTGCTTG
GTGCCTTGGCACTGGGTGATATTGGTCAGCACTTTCCAGATACAGATGTACAGTTTAAAGGTGCTGATAGCCGTGTACTG
CTTAAACACGTCTATCAACTCATTTTAGATCGTGGTTATGCTTTAAATAATGCTGATATTACAGTGGCATGTGAACGACC
AAAATTGGCAAAATATAATCTGGAGATGCGTCAAAGCATAGCAGATGTGCTGGATGTTGATGTCACTCAAATTAGTGTCA
AAGCCACAACCACTGAAAAACTAGGTTTTACAGGCAGACAAGAAGGTATTCTGGCAACCGCTACCGTATTGGTTTCCTAT
CAGGCAAAATGA

Upstream 100 bases:

>100_bases
CATGATCGCTTATACTTTCACTTGTCTTTGCTTCACGTACTATAGCGAAACCTAAAGCCAGAATACAAGATTTGTTAATT
ATTTTATAGAGGAATGAATA

Downstream 100 bases:

>100_bases
TCTGGATGAATCAGTGACTGTTCAATTTCTTGTGTTGCTTTACGGCCCTGAAGCTGTAAAGCAACACTATGAATGATGCC
TGTCCATGTTTCGTTTGGTT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MVVQIRIGQGMDVHAFEEGDHVTLAGIKIPHTHGLKAHSDGDVVLHALSDALLGALALGDIGQHFPDTDVQFKGADSRVL
LKHVYQLILDRGYALNNADITVACERPKLAKYNLEMRQSIADVLDVDVTQISVKATTTEKLGFTGRQEGILATATVLVSY
QAK

Sequences:

>Translated_163_residues
MVVQIRIGQGMDVHAFEEGDHVTLAGIKIPHTHGLKAHSDGDVVLHALSDALLGALALGDIGQHFPDTDVQFKGADSRVL
LKHVYQLILDRGYALNNADITVACERPKLAKYNLEMRQSIADVLDVDVTQISVKATTTEKLGFTGRQEGILATATVLVSY
QAK
>Mature_163_residues
MVVQIRIGQGMDVHAFEEGDHVTLAGIKIPHTHGLKAHSDGDVVLHALSDALLGALALGDIGQHFPDTDVQFKGADSRVL
LKHVYQLILDRGYALNNADITVACERPKLAKYNLEMRQSIADVLDVDVTQISVKATTTEKLGFTGRQEGILATATVLVSY
QAK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=54.5454545454545, Blast_Score=162, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_ACIAD (Q6FAU4)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046641.1
- ProteinModelPortal:   Q6FAU4
- SMR:   Q6FAU4
- STRING:   Q6FAU4
- GeneID:   2879845
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1996
- NMPDR:   fig|62977.3.peg.1903
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- PhylomeDB:   Q6FAU4
- ProtClustDB:   PRK00084
- BioCyc:   ASP62977:ACIAD1996-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17626; Mature: 17626

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVQIRIGQGMDVHAFEEGDHVTLAGIKIPHTHGLKAHSDGDVVLHALSDALLGALALGD
CEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IGQHFPDTDVQFKGADSRVLLKHVYQLILDRGYALNNADITVACERPKLAKYNLEMRQSI
HHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCEEECHHHHHHH
ADVLDVDVTQISVKATTTEKLGFTGRQEGILATATVLVSYQAK
HHHHCCCEEEEEEEEECCHHCCCCCCCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MVVQIRIGQGMDVHAFEEGDHVTLAGIKIPHTHGLKAHSDGDVVLHALSDALLGALALGD
CEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
IGQHFPDTDVQFKGADSRVLLKHVYQLILDRGYALNNADITVACERPKLAKYNLEMRQSI
HHCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCEEECHHHHHHH
ADVLDVDVTQISVKATTTEKLGFTGRQEGILATATVLVSYQAK
HHHHCCCEEEEEEEEECCHHCCCCCCCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA