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The map label for this gene is speE1

Identifier: 49188205

GI number: 49188205

Start: 5107467

End: 5108294

Strand: Reverse

Name: speE1

Synonym: BAS5219

Alternate gene names: 49188205

Gene position: 5108294-5107467 (Counterclockwise)

Preceding gene: 49188206

Following gene: 49188204

Centisome position: 97.7

GC content: 37.8

Gene sequence:

>828_bases
ATGGAACTATGGTTCACTGAAAAACAAACAAAACATTTTGGGATTACGGCGCGTATTAACCGCACATTACATACGGAGCA
AACAGAATTCCAAAAACTTGATATGGTTGAAACGGAAGAGTTCGGAAACATGCTTATTTTAGATGGCATGGTTATGACGA
CAGAAAAAGATGAGTTTGTGTACCATGAAATGGTAGCACACGTACCTTTATTTACACATCCAAACCCTGAAAACGTATTA
GTTGTTGGTGGCGGTGATGGCGGTGTTATTCGTGAAGTGTTAAAACACCCAAGCGTAAAGAAAGCAACTCTTGTTGAAAT
CGATGGAAAAGTAATTGAGTACTCTAAACAATACTTACCATCAATTGCAGGTGCATTAGATAATGAGCGTGTAGAAGTGA
AAGTAGGAGACGGTTTCCTACATATCGCAGAAAGCGAAAATGAATATGACGTAATTATGGTAGATTCTACTGAGCCAGTA
GGACCAGCAGTAAACCTATTTACAAAAGGCTTCTACGCTGGAATTTCAAAAGCGTTAAAAGAAGATGGTATTTTCGTTGC
ACAAACGGACAACCCTTGGTTTACACCAGAATTAATTACAACTGTGTTTAAAGACGTAAAAGAGATCTTCCCAATTACTC
GTTTATACACAGCGAACATTCCAACTTACCCAAGTGGTCTTTGGACGTTCACAATTGGATCTAAAAAACATGATCCATTA
GAAGTAAGTGAAGAGCGTTTCCACGAAATCGAAACGAAATACTATACAAAAGAATTACACAATGCAGCATTCGCATTACC
GAAATTTGTTGGCGATCTAATTAAGTAA

Upstream 100 bases:

>100_bases
GTACTTTTAACTATACATTTTGTATGTTTTGTTTTATTTTTGTTAGGGGACATTAATGATGTCCCTATTATAAATTTGCA
TTGAAAGGCGTGATTGTACG

Downstream 100 bases:

>100_bases
GAGAATCGCACGGAACAAATAAAGTGTAAGTAGCTCATTTAAGGTGTTTGTCCAGAAGACTTGAACATGCTAGCTATTTA
CACTTCTGTAAAGAAAATTG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKQYLPSIAGALDNERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITTVFKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKHDPL
EVSEERFHEIETKYYTKELHNAAFALPKFVGDLIK

Sequences:

>Translated_275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKQYLPSIAGALDNERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITTVFKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKHDPL
EVSEERFHEIETKYYTKELHNAAFALPKFVGDLIK
>Mature_275_residues
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFVYHEMVAHVPLFTHPNPENVL
VVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKQYLPSIAGALDNERVEVKVGDGFLHIAESENEYDVIMVDSTEPV
GPAVNLFTKGFYAGISKALKEDGIFVAQTDNPWFTPELITTVFKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKHDPL
EVSEERFHEIETKYYTKELHNAAFALPKFVGDLIK

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=277, Percent_Identity=38.2671480144404, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI1786312, Length=269, Percent_Identity=38.2899628252788, Blast_Score=210, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI25154106, Length=268, Percent_Identity=38.4328358208955, Blast_Score=201, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI212645616, Length=222, Percent_Identity=42.3423423423423, Blast_Score=199, Evalue=9e-52,
Organism=Saccharomyces cerevisiae, GI6325326, Length=277, Percent_Identity=39.3501805054152, Blast_Score=222, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6323175, Length=286, Percent_Identity=36.013986013986, Blast_Score=202, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24645443, Length=274, Percent_Identity=41.970802919708, Blast_Score=244, Evalue=5e-65,
Organism=Drosophila melanogaster, GI21355941, Length=211, Percent_Identity=43.1279620853081, Blast_Score=191, Evalue=6e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE1_BACAN (Q81JT0)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847770.1
- RefSeq:   YP_031458.1
- RefSeq:   YP_052657.1
- ProteinModelPortal:   Q81JT0
- SMR:   Q81JT0
- EnsemblBacteria:   EBBACT00000012133
- EnsemblBacteria:   EBBACT00000016227
- EnsemblBacteria:   EBBACT00000021818
- GeneID:   1085326
- GeneID:   2821015
- GeneID:   2848266
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5619
- KEGG:   bar:GBAA_5619
- KEGG:   bat:BAS5219
- TIGR:   BA_5619
- TIGR:   GBAA_5619
- GeneTree:   EBGT00050000002486
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- ProtClustDB:   PRK00811
- BioCyc:   BANT260799:BAS5219-MONOMER
- BioCyc:   BANT261594:GBAA5619-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31144; Mature: 31144

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 31-31 BINDING 86-86 BINDING 106-106 BINDING 155-155 BINDING 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFV
CCCEECCCCCCEECEEEEECCEECCCHHHHHHHCCEEHHHHCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKQYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
SIAGALDNERVEVKVGDGFLHIAESENEYDVIMVDSTEPVGPAVNLFTKGFYAGISKALK
HHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFTPELITTVFKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKHDPL
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCC
EVSEERFHEIETKYYTKELHNAAFALPKFVGDLIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELWFTEKQTKHFGITARINRTLHTEQTEFQKLDMVETEEFGNMLILDGMVMTTEKDEFV
CCCEECCCCCCEECEEEEECCEECCCHHHHHHHCCEEHHHHCCEEEECCEEEECCCHHHH
YHEMVAHVPLFTHPNPENVLVVGGGDGGVIREVLKHPSVKKATLVEIDGKVIEYSKQYLP
HHHHHHHCCEEECCCCCEEEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
SIAGALDNERVEVKVGDGFLHIAESENEYDVIMVDSTEPVGPAVNLFTKGFYAGISKALK
HHHHCCCCCEEEEEECCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
EDGIFVAQTDNPWFTPELITTVFKDVKEIFPITRLYTANIPTYPSGLWTFTIGSKKHDPL
HCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHEEECCCCCCCCCEEEEEECCCCCCCC
EVSEERFHEIETKYYTKELHNAAFALPKFVGDLIK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629