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The map label for this gene is speB

Identifier: 49188204

GI number: 49188204

Start: 5106377

End: 5107249

Strand: Reverse

Name: speB

Synonym: BAS5218

Alternate gene names: 49188204

Gene position: 5107249-5106377 (Counterclockwise)

Preceding gene: 49188205

Following gene: 49188202

Centisome position: 97.68

GC content: 40.21

Gene sequence:

>873_bases
ATGCGTTTTGATGAAGCTTATTCAGGTAAAGTATTTATTAAAAGTCATCCAAGTTTTGAAGAGTCAGAGGTAGTTATTTA
CGGGATGCCTATGGATTGGACAGTAAGTTACCGTCCAGGATCTCGCTTTGGCCCTGCACGTATTCGTGAAGTATCAATCG
GTCTTGAAGAATATAGTCCGTATTTAGATCGTGAACTAGAAGAGGTAAAATATTTTGATGCGGGTGATATCCCATTACCA
TTTGGAAATGCACAACGCAGCTTAGACATGATTGAAGAATATGTATCAAAACTTTTAGATGCCGGTAAGTTTCCACTAGG
TCTCGGCGGTGAGCACCTAGTGTCTTGGCCAATTTTTAAGGCAATGGCAAAAAAATATCCGGATTTAGCAATCATCCATA
TGGATGCTCATACTGATTTACGTGAATCGTATGAAGGGGAGCCTTTATCCCACTCTACACCTATTCGTAAAGTGTGTGAT
TTAATTGGTCCGGAAAACGTATATTCTTTCGGAATTCGTTCTGGAATGAAGGAAGAATTTGAATGGGCGAAAGAAGTAGG
TATGAACTTATACAAATTTGACGTATTAGAACCGTTAAAAGAAGTATTACCGAAACTTGAGGGACGCCCAGTCTATGTCA
CAATCGACATTGATGTATTAGACCCAGCTCACGCTCCAGGAACAGGAACGTTAGAAGCTGGTGGTATCACATCTAAAGAA
TTATTAGATTCCATCGTAGCAATTGCAAATTCAAATATAAATGTAGTTGGAGCAGACTTAGTAGAAGTAGCTCCTGTCTA
CGACCATAGTGATCAAACACCAGTCGCAGCAAGCAAATTCGTGCGGGAAATGCTGCTCGGTTGGGTAAAGTAA

Upstream 100 bases:

>100_bases
AGAAGACCCGGTAAATCGAGAAAGAGGAGCAACTCGCTCCAAATTTGAGAAATAATGAAAGGTTGGGATACCTTAAGTTC
CACATAAGGAGGAAAAGAAT

Downstream 100 bases:

>100_bases
AAGCGGAATCGGCTCGCCCAGAATGTGAGGGGGATGGAGCTTCAGACATAGAGGCGTTTTTTGCCTCGAAGGAAGAAGCG
AAGCCACCGATCATTCTAGC

Product: agmatinase

Products: NA

Alternate protein names: Agmatine ureohydrolase; AUH

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MRFDEAYSGKVFIKSHPSFEESEVVIYGMPMDWTVSYRPGSRFGPARIREVSIGLEEYSPYLDRELEEVKYFDAGDIPLP
FGNAQRSLDMIEEYVSKLLDAGKFPLGLGGEHLVSWPIFKAMAKKYPDLAIIHMDAHTDLRESYEGEPLSHSTPIRKVCD
LIGPENVYSFGIRSGMKEEFEWAKEVGMNLYKFDVLEPLKEVLPKLEGRPVYVTIDIDVLDPAHAPGTGTLEAGGITSKE
LLDSIVAIANSNINVVGADLVEVAPVYDHSDQTPVAASKFVREMLLGWVK

Sequences:

>Translated_290_residues
MRFDEAYSGKVFIKSHPSFEESEVVIYGMPMDWTVSYRPGSRFGPARIREVSIGLEEYSPYLDRELEEVKYFDAGDIPLP
FGNAQRSLDMIEEYVSKLLDAGKFPLGLGGEHLVSWPIFKAMAKKYPDLAIIHMDAHTDLRESYEGEPLSHSTPIRKVCD
LIGPENVYSFGIRSGMKEEFEWAKEVGMNLYKFDVLEPLKEVLPKLEGRPVYVTIDIDVLDPAHAPGTGTLEAGGITSKE
LLDSIVAIANSNINVVGADLVEVAPVYDHSDQTPVAASKFVREMLLGWVK
>Mature_290_residues
MRFDEAYSGKVFIKSHPSFEESEVVIYGMPMDWTVSYRPGSRFGPARIREVSIGLEEYSPYLDRELEEVKYFDAGDIPLP
FGNAQRSLDMIEEYVSKLLDAGKFPLGLGGEHLVSWPIFKAMAKKYPDLAIIHMDAHTDLRESYEGEPLSHSTPIRKVCD
LIGPENVYSFGIRSGMKEEFEWAKEVGMNLYKFDVLEPLKEVLPKLEGRPVYVTIDIDVLDPAHAPGTGTLEAGGITSKE
LLDSIVAIANSNINVVGADLVEVAPVYDHSDQTPVAASKFVREMLLGWVK

Specific function: Catalyzes the formation of putrescine from agmatine

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family. Agmatinase subfamily

Homologues:

Organism=Homo sapiens, GI37537722, Length=284, Percent_Identity=36.9718309859155, Blast_Score=181, Evalue=6e-46,
Organism=Homo sapiens, GI10947139, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789306, Length=284, Percent_Identity=35.9154929577465, Blast_Score=151, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6325146, Length=230, Percent_Identity=28.695652173913, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEB_BACAN (Q81JT1)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847769.1
- RefSeq:   YP_022289.1
- RefSeq:   YP_031457.1
- ProteinModelPortal:   Q81JT1
- SMR:   Q81JT1
- EnsemblBacteria:   EBBACT00000008654
- EnsemblBacteria:   EBBACT00000014000
- EnsemblBacteria:   EBBACT00000020632
- GeneID:   1085325
- GeneID:   2817842
- GeneID:   2848265
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5617
- KEGG:   bar:GBAA_5617
- KEGG:   bat:BAS5218
- TIGR:   BA_5617
- TIGR:   GBAA_5617
- GeneTree:   EBGT00050000001627
- HOGENOM:   HBG391953
- OMA:   RARWRED
- ProtClustDB:   CLSK888062
- BioCyc:   BANT260799:BAS5218-MONOMER
- BioCyc:   BANT261594:GBAA5617-MONOMER
- BRENDA:   3.5.3.11
- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855
- Gene3D:   G3DSA:3.40.800.10
- PANTHER:   PTHR11358
- TIGRFAMs:   TIGR01230

Pfam domain/function: PF00491 Arginase

EC number: =3.5.3.11

Molecular weight: Translated: 32390; Mature: 32390

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS01053 ARGINASE_1; PS51409 ARGINASE_2; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDEAYSGKVFIKSHPSFEESEVVIYGMPMDWTVSYRPGSRFGPARIREVSIGLEEYSP
CCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHEECCHHHHCC
YLDRELEEVKYFDAGDIPLPFGNAQRSLDMIEEYVSKLLDAGKFPLGLGGEHLVSWPIFK
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AMAKKYPDLAIIHMDAHTDLRESYEGEPLSHSTPIRKVCDLIGPENVYSFGIRSGMKEEF
HHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHH
EWAKEVGMNLYKFDVLEPLKEVLPKLEGRPVYVTIDIDVLDPAHAPGTGTLEAGGITSKE
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCHHH
LLDSIVAIANSNINVVGADLVEVAPVYDHSDQTPVAASKFVREMLLGWVK
HHHHHHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRFDEAYSGKVFIKSHPSFEESEVVIYGMPMDWTVSYRPGSRFGPARIREVSIGLEEYSP
CCCCCCCCCEEEEECCCCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHEECCHHHHCC
YLDRELEEVKYFDAGDIPLPFGNAQRSLDMIEEYVSKLLDAGKFPLGLGGEHLVSWPIFK
HHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
AMAKKYPDLAIIHMDAHTDLRESYEGEPLSHSTPIRKVCDLIGPENVYSFGIRSGMKEEF
HHHHHCCCEEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHH
EWAKEVGMNLYKFDVLEPLKEVLPKLEGRPVYVTIDIDVLDPAHAPGTGTLEAGGITSKE
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEECCCCCHHH
LLDSIVAIANSNINVVGADLVEVAPVYDHSDQTPVAASKFVREMLLGWVK
HHHHHHHHHCCCCEEEECHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629