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The map label for this gene is ywjE [H]

Identifier: 49188181

GI number: 49188181

Start: 5077180

End: 5078373

Strand: Direct

Name: ywjE [H]

Synonym: BAS5195

Alternate gene names: 49188181

Gene position: 5077180-5078373 (Clockwise)

Preceding gene: 49188172

Following gene: 49188182

Centisome position: 97.1

GC content: 34.92

Gene sequence:

>1194_bases
ATGTTCTTCTTACCTCTCTTATTAATAGGTATTGCTCTCTGGATTATAATCGATCTTTCTTATGGGAGAATTGTTCATTT
AAGGCGTGTACGTTCTCGTTCTTTCCCTTTACGTCAAAGTGATTTTCACCTTTATACATACGGAAAAGATTTGTATGACA
CTCTATTTACTGATATAAAACAGGCGAAACACCATGTGCATGTTTTGTTCTTCATTGTAAAAAACGATAAAATCAGCCGG
GAATTTTTAAAGTTACTTATTGATAAGGCAAAGGAAGGAATTGAAGTAAGACTTTTACTCGATAGATTGGGAAGCCATCA
TTTATCAAACGAGGCCATTCGCTCCCTGCAAAAACATGGTGTCTCTTTTTCATTTTGTCACAAAGTAAAGTTTCCTTTTC
CTTTCTTTTCTGCAAATCAAAGAAACCATAGAAAAATTACAGTTATCGACGGGAAAATTGGTTACATTGGGGGATTTAAT
ATTGGTGAAGAGTATTTGGGACATAATGAACGGTTAGGATTATGGAGGGACTATCATTTACGTCTTACAGGCGAAGGAGT
ACAAGATTTACAAAAGCAATTTTTACATGACTGGTTTGATGATACAAACCAAAATTTATTGGTCGCTTCTCTTTATTTTC
CGAAACAGCAACCCGGTACTATCCAACATCAATTTATTCCGACTGATGGTGCCTATTTACAACATACTTTTTTACATTTA
ATTGAAGGGGCAAAAAAGGAACTCTTTATCGGCACACCCTATTTCATTCCTGGAAGGAAAATTATGAACGCATTATTAGA
AGCACGAAAACGCGGGGTTCAAATCACGATTCTCGTTCCAGAAAAAGCGGATCATGCCCTTGTTCGAGAAGCAAAATTCC
CGTATTGCCGAAAGTTAATACAAGCTGGATGTAATATTTACGCATTTCAACAAGGTTTTTTTCACGCTAAAGTTATTATA
GTTGATGACTACATTTGTGATATCGGAACTGCAAACTTTGATATGAGAAGTTTATATATTAACCATGAAATTAACTGCCT
TTTATATGATAAACATTTTATACAAACAGTAAGAAACAAATTCCATAAGGATCTAGGAAACGCATCATTACTTTCCTTTA
AAGATGTTAGCCCGCATTCTCTTATTGATAGAGGAAAAGAATGGATAGGAACAATACTCGCTTTCTTCCTATAG

Upstream 100 bases:

>100_bases
TATTCCCTACTGATTATTAGCCTGCAAATAACAGGATAATATGAATGTTTTATTTATAAAAAACGAATGGTGAAAAGACC
GTGAAGGAGGGAATTTGCTC

Downstream 100 bases:

>100_bases
GAAAGATAATGCATAAAGGATGTGTATTCATTATGATTATGCGGTTCGGATATGTCTCACATGCAATGGCACTCTGGGAC
TGCTCTCCGGCTAAAACGAT

Product: cardiolipin synthetase domain-containing protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MFFLPLLLIGIALWIIIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHVLFFIVKNDKISR
EFLKLLIDKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLVASLYFPKQQPGTIQHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL

Sequences:

>Translated_397_residues
MFFLPLLLIGIALWIIIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHVLFFIVKNDKISR
EFLKLLIDKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLVASLYFPKQQPGTIQHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
>Mature_397_residues
MFFLPLLLIGIALWIIIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIKQAKHHVHVLFFIVKNDKISR
EFLKLLIDKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHGVSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFN
IGEEYLGHNERLGLWRDYHLRLTGEGVQDLQKQFLHDWFDDTNQNLLVASLYFPKQQPGTIQHQFIPTDGAYLQHTFLHL
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLIQAGCNIYAFQQGFFHAKVII
VDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNKFHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=328, Percent_Identity=29.2682926829268, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787007, Length=333, Percent_Identity=27.6276276276276, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI87081826, Length=404, Percent_Identity=23.019801980198, Blast_Score=82, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46213; Mature: 46213

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50035 PLD ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFLPLLLIGIALWIIIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIK
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
QAKHHVHVLFFIVKNDKISREFLKLLIDKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHG
HHCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHCC
VSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFNIGEEYLGHNERLGLWRDYHL
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHCCCCCCCCCEEEEEE
RLTGEGVQDLQKQFLHDWFDDTNQNLLVASLYFPKQQPGTIQHQFIPTDGAYLQHTFLHL
EEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLI
HCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
QAGCNIYAFQQGFFHAKVIIVDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNK
HCCCCEEEEECCCEEEEEEEEECEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHH
FHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MFFLPLLLIGIALWIIIDLSYGRIVHLRRVRSRSFPLRQSDFHLYTYGKDLYDTLFTDIK
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHH
QAKHHVHVLFFIVKNDKISREFLKLLIDKAKEGIEVRLLLDRLGSHHLSNEAIRSLQKHG
HHCCEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHCC
VSFSFCHKVKFPFPFFSANQRNHRKITVIDGKIGYIGGFNIGEEYLGHNERLGLWRDYHL
CCEEEEEEECCCCCCCCCCCCCCCEEEEEECCEEEEECCCCCHHHCCCCCCCCCEEEEEE
RLTGEGVQDLQKQFLHDWFDDTNQNLLVASLYFPKQQPGTIQHQFIPTDGAYLQHTFLHL
EEECCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHH
IEGAKKELFIGTPYFIPGRKIMNALLEARKRGVQITILVPEKADHALVREAKFPYCRKLI
HCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHCCCHHHHHHH
QAGCNIYAFQQGFFHAKVIIVDDYICDIGTANFDMRSLYINHEINCLLYDKHFIQTVRNK
HCCCCEEEEECCCEEEEEEEEECEEECCCCCCCEEEEEEECCCEEEEEECHHHHHHHHHH
FHKDLGNASLLSFKDVSPHSLIDRGKEWIGTILAFFL
HHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]