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The map label for this gene is gap1

Identifier: 49187975

GI number: 49187975

Start: 4864685

End: 4865689

Strand: Reverse

Name: gap1

Synonym: BAS4989

Alternate gene names: 49187975

Gene position: 4865689-4864685 (Counterclockwise)

Preceding gene: 49187976

Following gene: 49187974

Centisome position: 93.06

GC content: 39.8

Gene sequence:

>1005_bases
ATGACTAAAATTGGTATTAATGGATTTGGACGTATCGGACGTAACGTATTCCGCGCAGCTCTTAACAACTCTGAGGTAGA
AGTAGTAGCAATCAACGACTTAACAGATGCTAAAACATTAGCTCACCTTTTAAAATATGACACAGTTCACGGAACTTTAA
ATGCAGAAGTATCTGCTAACGAAAACAGCATCGTTGTTAACGGTAAAGAAATTAAAGTTATCGCTGAGCGTGACCCAGCT
CAATTACCATGGAGCGACTACGGAGTAGAAGTAGTAGTAGAATCTACTGGCCGTTTCACTAAAAAATCAGACGCTGAAAA
ACACTTAGGTGGATCAGTTAAGAAAGTTATCATCTCAGCTCCAGCTTCTGACGAAGATATCACTGTAGTTATGGGTGTTA
ACCACGAACAATACGATGCAGCTAACCACAACGTAGTATCTAACGCTTCTTGTACTACAAACTGTCTAGCTCCATTCGCT
AAAGTATTAAACGAAAAATTCGGCGTAAAACGCGGAATGATGACAACAATTCACTCTTACACTAACGACCAACAAATCTT
AGACTTACCACACAAAGATTTACGTCGTGCTCGTGCAGCAGCTGAAAACATGATCCCAACATCTACTGGTGCAGCTAAAG
CTGTAGCATTAGTATTACCAGAACTTAAAGGTAAATTAAACGGTGGCGCTGTACGTGTTCCAACTGCTAACGTTTCTCTA
GTTGACCTAGTTGTTGAACTTGACAAAGAAGTAACAGTTGAAGAAGTAAATGCAGCATTCAAAGCAGCAGCTGAAGGCGA
ATTAAAAGGTATCCTTGGATACAGCGAAGAGCCATTAGTATCTATCGACTATAACGGATGTACAGCTTCTTCTACAATCG
ATGCATTATCTACAATGGTAATGGAAGGTAACATGGTTAAAGTACTTTCTTGGTACGATAACGAAACAGGTTACTCTAAC
CGCGTAGTAGACTTAGCAGCTTACATGACTTCAAAAGGTCTTTAA

Upstream 100 bases:

>100_bases
AACAAGGGCACACTTCAATTCTAATTACAGATGAAGGTGCAGCAAAACAGTTAACAAAGGGTATTACCCTTTAATATAAT
CCCCCAAGGAGGAAATTCAA

Downstream 100 bases:

>100_bases
TTCTTAATTATATAAGTTATCAATGACAAAACGAGGGAGAGGGTTTGTTCCCTCTCTCTCTTCTTTCGTTTTAAAAGCAA
ATGTGTTAAGCTTTTGAGTG

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH 1

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTKIGINGFGRIGRNVFRAALNNSEVEVVAINDLTDAKTLAHLLKYDTVHGTLNAEVSANENSIVVNGKEIKVIAERDPA
QLPWSDYGVEVVVESTGRFTKKSDAEKHLGGSVKKVIISAPASDEDITVVMGVNHEQYDAANHNVVSNASCTTNCLAPFA
KVLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSL
VDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWYDNETGYSN
RVVDLAAYMTSKGL

Sequences:

>Translated_334_residues
MTKIGINGFGRIGRNVFRAALNNSEVEVVAINDLTDAKTLAHLLKYDTVHGTLNAEVSANENSIVVNGKEIKVIAERDPA
QLPWSDYGVEVVVESTGRFTKKSDAEKHLGGSVKKVIISAPASDEDITVVMGVNHEQYDAANHNVVSNASCTTNCLAPFA
KVLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSL
VDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWYDNETGYSN
RVVDLAAYMTSKGL
>Mature_333_residues
TKIGINGFGRIGRNVFRAALNNSEVEVVAINDLTDAKTLAHLLKYDTVHGTLNAEVSANENSIVVNGKEIKVIAERDPAQ
LPWSDYGVEVVVESTGRFTKKSDAEKHLGGSVKKVIISAPASDEDITVVMGVNHEQYDAANHNVVSNASCTTNCLAPFAK
VLNEKFGVKRGMMTTIHSYTNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSLV
DLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMVMEGNMVKVLSWYDNETGYSNR
VVDLAAYMTSKGL

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI7669492, Length=332, Percent_Identity=52.710843373494, Blast_Score=350, Evalue=1e-96,
Organism=Homo sapiens, GI7657116, Length=331, Percent_Identity=48.9425981873112, Blast_Score=319, Evalue=3e-87,
Organism=Escherichia coli, GI1788079, Length=329, Percent_Identity=54.7112462006079, Blast_Score=377, Evalue=1e-106,
Organism=Escherichia coli, GI1789295, Length=333, Percent_Identity=44.4444444444444, Blast_Score=297, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI17534677, Length=337, Percent_Identity=51.3353115727003, Blast_Score=335, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI17534679, Length=337, Percent_Identity=50.7418397626113, Blast_Score=333, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI32566163, Length=342, Percent_Identity=50.8771929824561, Blast_Score=330, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI17568413, Length=337, Percent_Identity=50.7418397626113, Blast_Score=328, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=52.2658610271903, Blast_Score=343, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=51.6616314199396, Blast_Score=343, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=51.6616314199396, Blast_Score=342, Evalue=4e-95,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=52.3952095808383, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=52.3952095808383, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=52.3952095808383, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=52.3952095808383, Blast_Score=330, Evalue=6e-91,
Organism=Drosophila melanogaster, GI19922412, Length=330, Percent_Identity=49.6969696969697, Blast_Score=328, Evalue=3e-90,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): G3P1_BACCE (Q4MQ58)

Other databases:

- EMBL:   AAEK01000016
- HSSP:   P00362
- ProteinModelPortal:   Q4MQ58
- SMR:   Q4MQ58
- BRENDA:   1.2.1.12
- GO:   GO:0005737
- GO:   GO:0006096
- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR10836
- PIRSF:   PIRSF000149
- PRINTS:   PR00078
- SMART:   SM00846
- TIGRFAMs:   TIGR01534

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N

EC number: =1.2.1.12

Molecular weight: Translated: 35826; Mature: 35695

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00071 GAPDH

Important sites: ACT_SITE 151-151 BINDING 33-33 BINDING 77-77 BINDING 119-119 BINDING 181-181 BINDING 196-196 BINDING 232-232 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIGINGFGRIGRNVFRAALNNSEVEVVAINDLTDAKTLAHLLKYDTVHGTLNAEVSAN
CCEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECC
ENSIVVNGKEIKVIAERDPAQLPWSDYGVEVVVESTGRFTKKSDAEKHLGGSVKKVIISA
CCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEEEEC
PASDEDITVVMGVNHEQYDAANHNVVSNASCTTNCLAPFAKVLNEKFGVKRGMMTTIHSY
CCCCCCEEEEEECCCHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHC
TNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSL
CCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
VDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMV
HHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHH
MEGNMVKVLSWYDNETGYSNRVVDLAAYMTSKGL
HCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKIGINGFGRIGRNVFRAALNNSEVEVVAINDLTDAKTLAHLLKYDTVHGTLNAEVSAN
CEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECC
ENSIVVNGKEIKVIAERDPAQLPWSDYGVEVVVESTGRFTKKSDAEKHLGGSVKKVIISA
CCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHCCCEEEEEEEC
PASDEDITVVMGVNHEQYDAANHNVVSNASCTTNCLAPFAKVLNEKFGVKRGMMTTIHSY
CCCCCCEEEEEECCCHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHC
TNDQQILDLPHKDLRRARAAAENMIPTSTGAAKAVALVLPELKGKLNGGAVRVPTANVSL
CCCCCEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCHH
VDLVVELDKEVTVEEVNAAFKAAAEGELKGILGYSEEPLVSIDYNGCTASSTIDALSTMV
HHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHH
MEGNMVKVLSWYDNETGYSNRVVDLAAYMTSKGL
HCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA