BacMap

Definition	Bacillus anthracis str. Sterne chromosome, complete genome.
Accession	NC_005945
Length	5,228,663

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The map label for this gene is ytbE [H]

Identifier: 49187919

GI number: 49187919

Start: 4813637

End: 4814476

Strand: Reverse

Name: ytbE [H]

Synonym: BAS4931

Alternate gene names: 49187919

Gene position: 4814476-4813637 (Counterclockwise)

Preceding gene: 49187920

Following gene: 49187918

Centisome position: 92.08

GC content: 35.48

Gene sequence:

>840_bases
ATGAAAAACTTACAAAGTAAAGCAGTATTAAATAACGGTGTAGAAATGCCTTGGTTCGGTTTAGGTGTATTTAAAGTAGA
AGAAGGACCAGAACTTGTAGAAGCTGTAAAATCAGCAATTAAAGCAGGATACCGCAGCATCGATACAGCTGCAATTTACG
GAAATGAAAAAGCTGTAGGTGAAGGAATCCGTGCAGGTATCGAAGCAACTGGTATTTCAAGAGAAGATTTATTCATCACT
TCAAAAGTATGGAACGCAGATCAAGGATATGAAGAAACAATTGCTGCATATGAAGAGAGCTTAAAAAAATTAGAACTAGA
TTATTTAGATCTGTATCTTGTTCACTGGCCTGTAGAAGGAAAATATAAAGATACATGGAGAGCGTTAGAAACACTTTATA
AAGAAAAGCGCGTACGTGCAATTGGTGTAAGTAACTTCCAAGTACATCACTTACAAGATGTAATGAAAGATGCAGAAATT
AAACCAATGATTAACCAAGTAGAATACCACCCTCGTTTAACACAAAAAGAAGTACAAGCTTTCTGTAAAGAACAAGGTAT
TCAAATGGAAGCATGGTCACCATTAATGCAAGGTCAATTATTAGATAACGAAACATTACAAGAGATTGCTGAGAAACACG
GTAAAACAACAGCGCAAGTTATTTTACGTTGGGATCTTCAAAATGGAGTAATTACAATTCCAAAATCAACGAAAGAACAC
CGCATTATTGCAAACGCTGATGTATTTAACTTTGAATTAACGAAAGAAGATATGGAAAAAATCGATGCGTTAAACCAAAA
TCACCGCGTGGGTCCAGATCCTGATAACTTCGATTTCTAA

Upstream 100 bases:

>100_bases
TGGGTAGGCGGCCTTATGGTAGTAGCCGCTGTTATTTTAACAGCGTGGAGCATGACATTAGAAAAAAGAGATCAAGTAAA
ATAAAAGGAGAGAAAATAAA

Downstream 100 bases:

>100_bases
GATTAAGGTAAGAAAAAAGAGTGCGTAAAAGCACTCTTTTTTTGTGCATAAAAACAGTTTCTAAATAAATACTACTTAGT
ATTAAGTTTGTTATTAAGGG

Product: aldo/keto reductase family oxidoreductase

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGISREDLFIT
SKVWNADQGYEETIAAYEESLKKLELDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQVHHLQDVMKDAEI
KPMINQVEYHPRLTQKEVQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Sequences:

>Translated_279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGISREDLFIT
SKVWNADQGYEETIAAYEESLKKLELDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQVHHLQDVMKDAEI
KPMINQVEYHPRLTQKEVQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
>Mature_279_residues
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVGEGIRAGIEATGISREDLFIT
SKVWNADQGYEETIAAYEESLKKLELDYLDLYLVHWPVEGKYKDTWRALETLYKEKRVRAIGVSNFQVHHLQDVMKDAEI
KPMINQVEYHPRLTQKEVQAFCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=295, Percent_Identity=38.9830508474576, Blast_Score=222, Evalue=2e-58,
Organism=Homo sapiens, GI5174391, Length=295, Percent_Identity=38.9830508474576, Blast_Score=222, Evalue=2e-58,
Organism=Homo sapiens, GI223468663, Length=292, Percent_Identity=40.4109589041096, Blast_Score=204, Evalue=7e-53,
Organism=Homo sapiens, GI93277124, Length=305, Percent_Identity=37.3770491803279, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI310109922, Length=290, Percent_Identity=39.3103448275862, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI310109920, Length=310, Percent_Identity=38.3870967741935, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI5453543, Length=310, Percent_Identity=38.3870967741935, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI45446745, Length=310, Percent_Identity=38.3870967741935, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI4503285, Length=310, Percent_Identity=38.3870967741935, Blast_Score=196, Evalue=1e-50,
Organism=Homo sapiens, GI24497585, Length=295, Percent_Identity=38.3050847457627, Blast_Score=192, Evalue=3e-49,
Organism=Homo sapiens, GI291291012, Length=278, Percent_Identity=39.568345323741, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI24497583, Length=293, Percent_Identity=37.542662116041, Blast_Score=187, Evalue=6e-48,
Organism=Homo sapiens, GI4502049, Length=301, Percent_Identity=39.8671096345515, Blast_Score=182, Evalue=2e-46,
Organism=Homo sapiens, GI5174695, Length=296, Percent_Identity=35.472972972973, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI300116273, Length=275, Percent_Identity=34.1818181818182, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI300116271, Length=274, Percent_Identity=33.9416058394161, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=47.3684210526316, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=47.3684210526316, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI310109924, Length=159, Percent_Identity=37.1069182389937, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87082198, Length=272, Percent_Identity=43.75, Blast_Score=244, Evalue=3e-66,
Organism=Escherichia coli, GI1786400, Length=263, Percent_Identity=36.8821292775665, Blast_Score=168, Evalue=3e-43,
Organism=Escherichia coli, GI1788081, Length=276, Percent_Identity=33.695652173913, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788070, Length=224, Percent_Identity=25.4464285714286, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI87081735, Length=282, Percent_Identity=23.4042553191489, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787674, Length=274, Percent_Identity=25.5474452554745, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71998625, Length=272, Percent_Identity=40.8088235294118, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17564128, Length=296, Percent_Identity=36.8243243243243, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI17537077, Length=292, Percent_Identity=40.0684931506849, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=39.202657807309, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17561298, Length=271, Percent_Identity=36.1623616236162, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17538386, Length=271, Percent_Identity=37.6383763837638, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17537079, Length=308, Percent_Identity=33.7662337662338, Blast_Score=180, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17550248, Length=300, Percent_Identity=36.6666666666667, Blast_Score=180, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17552492, Length=273, Percent_Identity=32.2344322344322, Blast_Score=177, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17562292, Length=274, Percent_Identity=32.1167883211679, Blast_Score=163, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=34.6863468634686, Blast_Score=161, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17566692, Length=287, Percent_Identity=32.7526132404181, Blast_Score=153, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17550246, Length=87, Percent_Identity=42.5287356321839, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321896, Length=317, Percent_Identity=36.5930599369085, Blast_Score=204, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6320576, Length=300, Percent_Identity=35.6666666666667, Blast_Score=189, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=35.7692307692308, Blast_Score=182, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6324694, Length=289, Percent_Identity=36.3321799307958, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319625, Length=296, Percent_Identity=36.8243243243243, Blast_Score=178, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6320079, Length=287, Percent_Identity=34.8432055749129, Blast_Score=145, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24663317, Length=292, Percent_Identity=40.4109589041096, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=37.7926421404682, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=37.9661016949153, Blast_Score=198, Evalue=3e-51,
Organism=Drosophila melanogaster, GI24657054, Length=290, Percent_Identity=38.2758620689655, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=35.423197492163, Blast_Score=194, Evalue=5e-50,
Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=35.8108108108108, Blast_Score=187, Evalue=5e-48,
Organism=Drosophila melanogaster, GI24662785, Length=296, Percent_Identity=41.8918918918919, Blast_Score=181, Evalue=6e-46,
Organism=Drosophila melanogaster, GI24662781, Length=293, Percent_Identity=41.9795221843003, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21356425, Length=291, Percent_Identity=34.7079037800687, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI45553081, Length=289, Percent_Identity=33.2179930795848, Blast_Score=173, Evalue=1e-43,
Organism=Drosophila melanogaster, GI281366140, Length=256, Percent_Identity=32.421875, Blast_Score=150, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 31823; Mature: 31823

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
EGIRAGIEATGISREDLFITSKVWNADQGYEETIAAYEESLKKLELDYLDLYLVHWPVEG
HHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQVHHLQDVMKDAEIKPMINQVEYHPRLTQKEVQA
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLQSKAVLNNGVEMPWFGLGVFKVEEGPELVEAVKSAIKAGYRSIDTAAIYGNEKAVG
CCCCHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEECCCHHHH
EGIRAGIEATGISREDLFITSKVWNADQGYEETIAAYEESLKKLELDYLDLYLVHWPVEG
HHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCC
KYKDTWRALETLYKEKRVRAIGVSNFQVHHLQDVMKDAEIKPMINQVEYHPRLTQKEVQA
CHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCHHHHHH
FCKEQGIQMEAWSPLMQGQLLDNETLQEIAEKHGKTTAQVILRWDLQNGVITIPKSTKEH
HHHHCCCCCHHCCHHHCCCCCCHHHHHHHHHHHCCCEEHEEEEEECCCCEEECCCCCCCC
RIIANADVFNFELTKEDMEKIDALNQNHRVGPDPDNFDF
EEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]