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The map label for this gene is metG1 [H]

Identifier: 49187891

GI number: 49187891

Start: 4783661

End: 4785295

Strand: Reverse

Name: metG1 [H]

Synonym: BAS4903

Alternate gene names: 49187891

Gene position: 4785295-4783661 (Counterclockwise)

Preceding gene: 49187895

Following gene: 49187885

Centisome position: 91.52

GC content: 37.49

Gene sequence:

>1635_bases
ATGAGTATTTTTATTGGGGGCGCTTGGCCGTATGCAAATGGTTCGTTACATCTTGGACATATTGCGAGTTTGTTACCTGG
AGATATTTTAGCACGTTATTACCGGGCAAAAGGTGAGAATGTGTTGTATGTTTCGGGGAGTGATTGCAATGGAACACCAA
TTGCAATTCGGGCAAAACAAGAAGGTGTGACGGCGAAGGAAATTGCTAATAAGTATCATGAAGAGTTTGAGCGATGTTTT
CGTAACCTTGGTTTTACGTATGATTGCTATACACGTACAGATAGTGAGCACCATCATGAAACGGTTCAAAATGTTTTTTT
ACGTTTATTAGAAGAAGGACATATTTATAAAAAAACAGTAGAACAAGCATATTGCGAAACGTGTACACAGTTTTTACCTG
ATTGTTATGTAGAAGGTGTTTGTCCACATTGCCATGAAGAGGCAAGAGGAGATCAATGCGATGCGTGTTCAGCTATTTTA
GATCCACTCGATTTGTTAGAAAAGAAATGTAAGTTATGTGGTAGTACACCATCCATACAGGAAACAGAGCATTTCTATTT
CGCATTGCATACATTTCAGGAGCAAATTAAAAGGGCTGTAGAAATCGCTAAACAAACAGGAACATGGCGCGACAATGCGA
TTCAACTAACAGAGAGATATGTAAAGGAAGGATTACTAGATAGGGCCGTATCACGTGATTTACCAATCGGGGTACCAATT
CCAGTAGAAGGGTATGAAGATAAGAAAATTTACGTATGGATTGAAGCAGTGACAGGGTATTATTCAGCAAGTAAACATTG
GGCTGAAGAAACAGGGAAAGATGATCGAGAGTTTTGGGATGGCGAAGCGAAAACATATTATGTGCACGGAAAGGATAATA
TTCCGTTTCATTCCGTCATTTGGCCAGCGGTCTTACTTGGAATAGGAGAAGGGACAATTCCTCGTCATATCGTTTCGAAT
GAATATTTAACAGTTGAAAAAAGAAAATTATCTACGAGTAAAAACTGGGCAGTATGGGTGCCTGATATATTAGAACGTTA
TGATCCAGATTCTATTCGTTACTTTTTAATAGTAAATGCACCAGAAAATCGTGACACTGACTTTTCATGGCGTGAATTTA
TTTACAGTCATAATAGTGAATTGTTAGGTGCATACGGGAACTTTGTGAACCGAACATTAAAGTTTATTGAAAAATATTAT
GGTGGTATCATGCCGAAGGGAAGTATTGATGTAGAGCTAAAAGATAAGATAGAAAGATTATATAAACATGTAGGAGAGGC
GATTGAGCAAACTAAATTTAAGGTGGCATTAGAATCAATATTTGATGCAGTACGTTTTGCAAATAAATACTTTGATGAGA
GGCAGCCATGGAAAGAAAGGGAAGATGATCCAGTTTCATGCGAAGAAACGATTTATAATTGTGTGTATCTCATCGCTAAT
TTTGCAAATCTCCTGGAACCATTTTTACCATTTTCAAGTGAAAGAATTAGAAACACATTATCGATTGTGAACCGAAATTG
GGAGCCTCAACATACGTTGCCAAGTAGAATTGATAGTGTACAGCCACTATTTGAACGAATCGATGTAAAACAAATTGAGT
GTGAGTTAGAAAAGCTATATGGAGCGGTAAAGTGA

Upstream 100 bases:

>100_bases
ATTGGCGAAATAAGGTGGTACCGCGACTGTTTATACAGCCCCGCCCTTATCTTTTTTAGATAAGGGCGGGGCTTTTTATA
TTTAAAAGGAGGAATGTAAG

Downstream 100 bases:

>100_bases
GATAGAGAAAAAGACCTTTCAAATGCATGAAAGGTCTTTTGTTATGCACTATGCACGCTACTATGTTTCTTTTCTTTCCT
AGCGGTAAGTAGCTGAGGAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase 1; MetRS 1 [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCF
RNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDCYVEGVCPHCHEEARGDQCDACSAIL
DPLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDGEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGTIPRHIVSN
EYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLIVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYY
GGIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPSRIDSVQPLFERIDVKQIECELEKLYGAVK

Sequences:

>Translated_544_residues
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCF
RNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDCYVEGVCPHCHEEARGDQCDACSAIL
DPLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDGEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGTIPRHIVSN
EYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLIVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYY
GGIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPSRIDSVQPLFERIDVKQIECELEKLYGAVK
>Mature_543_residues
SIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQEGVTAKEIANKYHEEFERCFR
NLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTVEQAYCETCTQFLPDCYVEGVCPHCHEEARGDQCDACSAILD
PLDLLEKKCKLCGSTPSIQETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPIP
VEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDGEAKTYYVHGKDNIPFHSVIWPAVLLGIGEGTIPRHIVSNE
YLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLIVNAPENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYG
GIMPKGSIDVELKDKIERLYKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIANF
ANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPSRIDSVQPLFERIDVKQIECELEKLYGAVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=33.2740213523132, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI24308436, Length=519, Percent_Identity=26.7822736030828, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI1788432, Length=546, Percent_Identity=30.03663003663, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1786861, Length=139, Percent_Identity=30.2158273381295, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=568, Percent_Identity=31.3380281690141, Blast_Score=313, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI212645096, Length=530, Percent_Identity=24.5283018867925, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=418, Percent_Identity=24.1626794258373, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=549, Percent_Identity=33.1511839708561, Blast_Score=323, Evalue=6e-89,
Organism=Saccharomyces cerevisiae, GI6321610, Length=497, Percent_Identity=24.748490945674, Blast_Score=109, Evalue=9e-25,
Organism=Drosophila melanogaster, GI24655311, Length=570, Percent_Identity=34.9122807017544, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI21358483, Length=519, Percent_Identity=24.6628131021195, Blast_Score=137, Evalue=1e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 62880; Mature: 62748

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQ
CEEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVTAKEIANKYHEEFERCFRNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
EQAYCETCTQFLPDCYVEGVCPHCHEEARGDQCDACSAILDPLDLLEKKCKLCGSTPSIQ
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCC
ETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDGEAKTYYVHGKDNIPFHSVI
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCHHHHH
WPAVLLGIGEGTIPRHIVSNEYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLIVNA
HHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCCEEECHHHHHCCCCCCEEEEEEEEC
PENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYGGIMPKGSIDVELKDKIERL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
YKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPSRIDSVQPLFERIDVKQIECELEKLY
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GAVK
CCCC
>Mature Secondary Structure 
SIFIGGAWPYANGSLHLGHIASLLPGDILARYYRAKGENVLYVSGSDCNGTPIAIRAKQ
EEEEECCCCCCCCCEEHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECC
EGVTAKEIANKYHEEFERCFRNLGFTYDCYTRTDSEHHHETVQNVFLRLLEEGHIYKKTV
CCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHH
EQAYCETCTQFLPDCYVEGVCPHCHEEARGDQCDACSAILDPLDLLEKKCKLCGSTPSIQ
HHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHCHHHHHHHHHHHCCCCCCCC
ETEHFYFALHTFQEQIKRAVEIAKQTGTWRDNAIQLTERYVKEGLLDRAVSRDLPIGVPI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PVEGYEDKKIYVWIEAVTGYYSASKHWAEETGKDDREFWDGEAKTYYVHGKDNIPFHSVI
CCCCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEEEECCCCCCHHHHH
WPAVLLGIGEGTIPRHIVSNEYLTVEKRKLSTSKNWAVWVPDILERYDPDSIRYFLIVNA
HHHHHHHCCCCCCCHHHHCCCCEEEEHHHCCCCCCCEEECHHHHHCCCCCCEEEEEEEEC
PENRDTDFSWREFIYSHNSELLGAYGNFVNRTLKFIEKYYGGIMPKGSIDVELKDKIERL
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHH
YKHVGEAIEQTKFKVALESIFDAVRFANKYFDERQPWKEREDDPVSCEETIYNCVYLIAN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
FANLLEPFLPFSSERIRNTLSIVNRNWEPQHTLPSRIDSVQPLFERIDVKQIECELEKLY
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
GAVK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA