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The map label for this gene is yugF [H]

Identifier: 49187764

GI number: 49187764

Start: 4666125

End: 4666964

Strand: Direct

Name: yugF [H]

Synonym: BAS4774

Alternate gene names: 49187764

Gene position: 4666125-4666964 (Clockwise)

Preceding gene: 49187763

Following gene: 49187766

Centisome position: 89.24

GC content: 36.55

Gene sequence:

>840_bases
ATGAATCACGAATATACTTGTCCTTATTTCACTTTTTCCACTCGCGGCACTACGATTCATTACGAATTGTATGAACATGA
TAATAAAACGGAACGCCCTACTTTTGTGCTCGTTCACGGCTTTTTATCTTCCTCATTCAGTTACCGCCGTCTCATTCCTT
TACTATCGAAAGAAGGAACCGTCATTGCTCTTGATTTACCACCGTTCGGAAAAAGTGATAAGTCTCATCTATTTAAGTAT
TCTTATCACAATTTAGCAACGATTATAATCGATTTAATTGAACACTTATCTCTCTCAAATATTGTGTTAGTTGGACATTC
TATGGGTGGACAAATTTCTCTTTATGTAAACCGTATACGTCCTGAATTAATTTCAAAGACAATTTTACTATGTAGCTCGA
GTTATTTAGCACGCGCAAACTTACCTTTAATGTATTCTTCTTATTTACCGTTCTTTCATTTATACGTAAAGAACTGGATT
ATACGGCGCGGCATCGTTCATAACTTAATGAATGTCGTTCATGATCATTCATTAATTGACGATGAAATGAAGGAAGGTTA
CTCTGCTCCTTTTTATGACAACCGTATATTCCCCGCTTTAACTCGTATGATACGAGATCGTGAAGGTGACTTATCTTCAA
CTGAATTACAAAAAATCGAAACACCTACGCTACTCATTTGGGGTGAAAAAGATCGCGTCGTTCCTGTACATGTAGGCCAT
CGTCTGCACAAAGATTTACCGAATTCGAAATTTATTTCTTACGAAAACACAGGACATTTACTACCTGAAGAAAAACCGGA
ACATGTTTATGAAGAAATTATCGCGTTTTCTGCGCAGTAA

Upstream 100 bases:

>100_bases
AACGAAAGGCAAGATGACATCGTCATCTTGCCTTTTTTTGTTAACATATTTTCTTTTCTCTTGGAGATGATATGAACAAA
AATCAATGGAGTGACAAAAA

Downstream 100 bases:

>100_bases
TGTAAAAGGCTGTCGGAAATTCCGACAGCCTACAATGAACAACTTCCGCCTTCTTTTATTACGAGTAATTCCACAGCTAA
CTTCTCACATTTTTCAAGCG

Product: alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNHEYTCPYFTFSTRGTTIHYELYEHDNKTERPTFVLVHGFLSSSFSYRRLIPLLSKEGTVIALDLPPFGKSDKSHLFKY
SYHNLATIIIDLIEHLSLSNIVLVGHSMGGQISLYVNRIRPELISKTILLCSSSYLARANLPLMYSSYLPFFHLYVKNWI
IRRGIVHNLMNVVHDHSLIDDEMKEGYSAPFYDNRIFPALTRMIRDREGDLSSTELQKIETPTLLIWGEKDRVVPVHVGH
RLHKDLPNSKFISYENTGHLLPEEKPEHVYEEIIAFSAQ

Sequences:

>Translated_279_residues
MNHEYTCPYFTFSTRGTTIHYELYEHDNKTERPTFVLVHGFLSSSFSYRRLIPLLSKEGTVIALDLPPFGKSDKSHLFKY
SYHNLATIIIDLIEHLSLSNIVLVGHSMGGQISLYVNRIRPELISKTILLCSSSYLARANLPLMYSSYLPFFHLYVKNWI
IRRGIVHNLMNVVHDHSLIDDEMKEGYSAPFYDNRIFPALTRMIRDREGDLSSTELQKIETPTLLIWGEKDRVVPVHVGH
RLHKDLPNSKFISYENTGHLLPEEKPEHVYEEIIAFSAQ
>Mature_279_residues
MNHEYTCPYFTFSTRGTTIHYELYEHDNKTERPTFVLVHGFLSSSFSYRRLIPLLSKEGTVIALDLPPFGKSDKSHLFKY
SYHNLATIIIDLIEHLSLSNIVLVGHSMGGQISLYVNRIRPELISKTILLCSSSYLARANLPLMYSSYLPFFHLYVKNWI
IRRGIVHNLMNVVHDHSLIDDEMKEGYSAPFYDNRIFPALTRMIRDREGDLSSTELQKIETPTLLIWGEKDRVVPVHVGH
RLHKDLPNSKFISYENTGHLLPEEKPEHVYEEIIAFSAQ

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=272, Percent_Identity=26.4705882352941, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI87081721, Length=288, Percent_Identity=25.3472222222222, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 32322; Mature: 32322

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHEYTCPYFTFSTRGTTIHYELYEHDNKTERPTFVLVHGFLSSSFSYRRLIPLLSKEGT
CCCCEECCEEEEECCCCEEEEEEEECCCCCCCCEEEEEEEHHCCCCCHHHHHHHHCCCCC
VIALDLPPFGKSDKSHLFKYSYHNLATIIIDLIEHLSLSNIVLVGHSMGGQISLYVNRIR
EEEEECCCCCCCCHHHEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEHHC
PELISKTILLCSSSYLARANLPLMYSSYLPFFHLYVKNWIIRRGIVHNLMNVVHDHSLID
HHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEMKEGYSAPFYDNRIFPALTRMIRDREGDLSSTELQKIETPTLLIWGEKDRVVPVHVGH
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCH
RLHKDLPNSKFISYENTGHLLPEEKPEHVYEEIIAFSAQ
HHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNHEYTCPYFTFSTRGTTIHYELYEHDNKTERPTFVLVHGFLSSSFSYRRLIPLLSKEGT
CCCCEECCEEEEECCCCEEEEEEEECCCCCCCCEEEEEEEHHCCCCCHHHHHHHHCCCCC
VIALDLPPFGKSDKSHLFKYSYHNLATIIIDLIEHLSLSNIVLVGHSMGGQISLYVNRIR
EEEEECCCCCCCCHHHEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEEHHC
PELISKTILLCSSSYLARANLPLMYSSYLPFFHLYVKNWIIRRGIVHNLMNVVHDHSLID
HHHHHHHHHHHCCCCCEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEMKEGYSAPFYDNRIFPALTRMIRDREGDLSSTELQKIETPTLLIWGEKDRVVPVHVGH
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCEEEEECCCCCEEEEECCH
RLHKDLPNSKFISYENTGHLLPEEKPEHVYEEIIAFSAQ
HHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377 [H]