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The map label for this gene is thiF [H]

Identifier: 49187613

GI number: 49187613

Start: 4516227

End: 4517240

Strand: Reverse

Name: thiF [H]

Synonym: BAS4620

Alternate gene names: 49187613

Gene position: 4517240-4516227 (Counterclockwise)

Preceding gene: 49187614

Following gene: 49187611

Centisome position: 86.39

GC content: 38.17

Gene sequence:

>1014_bases
ATGCAGGAGCGATATTCAAGGCAAATATTATTTTCTGGTGTTGGAGAAGAAGGACAGAGAAAAATTAGAGAGAAGCATGT
GCTTATTATTGGTGCTGGAGCTCTTGGTGCAGCAAATGCAGAAGCGATTGTTAGAGCAGGTGTTGGAAAAATAACAATTG
CGGATCGTGATTATGTAGAGTGGAGTAATTTACAAAGGCAACAATTATATACAGAAGAAGACGCAAAGCAGTATAAACCG
AAGGCGGTAGCAGCGGCAGAGTATTTAAAAGCAATTAATTCTGAGGTGGAAATAAATCCGGTTGTGACTGATGTAACAGT
GCAGGAAATGGAAGAGCTAGTGAAGGATGTAGATTTAATATTAGATGCGACAGATAATTTTGAAACGCGTCTTCTTATTA
ATGATATTTCACAAAAGTATAATATTCCGTGGATATATGGTGGTTGTGTCGGAAGCTACGGAGTAACGTATACAATTGTA
CCAGGGAAAACACCGTGTTTCCGCTGTTTAATGGAGCATCCAGCAAGCGGAGCAACATGTGATACGGCTGGTATTATACA
GCCGGCGGTGCAATTAGTAGTTGCGCATCAAATAACAGAAGCGTTGAAAATATTAGTAGAAGACTTTGAGGCACTTCGTG
AAACGATGTTATCGTTTGATCTTTGGAATAATCAACAGATGGCATTTAAAGTGAATAGACAGAAAAAAGACACGTGTTTA
TCTTGCGGAAGGTTACGTACATATCCGAGTTTAACTTTTGAAGCACAAACGAAAACAGAAGTGTTATGTGGCCGGAATAC
AGTACAAATCCGTCCAGGTGTGCGAAATGAATTTAATTTAGAAGAAATTAAAAAGCGCTTACAAAGAAGTGTGGATGTAA
AAGCTACTCCGTATTTAGTATCGTTTCTGGTAGAGGAATTTCGTTTTGTTTTATTTACAGATGGTAGGGCGTTTATTCAT
GGGACGAATGATATAAATGTAGCAAAACGTTTATATACAAGATACATAGGTTGA

Upstream 100 bases:

>100_bases
AGAAAAGATAGGTATTCGGATGAACGGACAGAAGAAAGTGCGAAAAATCGTCCGAAAATTGAAATGTCTTATATAGGAGG
ATAAGAAGGGAGGCTATCGT

Downstream 100 bases:

>100_bases
ACCTAAAGAGAAACCCTTTAGGTTCAACAGAGAGAAGATCAAGACTTTCTCGTATAATCTCCACTTTTTCCACCTGTTTT
TTCAAGTAAGTACGTTTCGC

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQERYSRQILFSGVGEEGQRKIREKHVLIIGAGALGAANAEAIVRAGVGKITIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAVAAAEYLKAINSEVEINPVVTDVTVQEMEELVKDVDLILDATDNFETRLLINDISQKYNIPWIYGGCVGSYGVTYTIV
PGKTPCFRCLMEHPASGATCDTAGIIQPAVQLVVAHQITEALKILVEDFEALRETMLSFDLWNNQQMAFKVNRQKKDTCL
SCGRLRTYPSLTFEAQTKTEVLCGRNTVQIRPGVRNEFNLEEIKKRLQRSVDVKATPYLVSFLVEEFRFVLFTDGRAFIH
GTNDINVAKRLYTRYIG

Sequences:

>Translated_337_residues
MQERYSRQILFSGVGEEGQRKIREKHVLIIGAGALGAANAEAIVRAGVGKITIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAVAAAEYLKAINSEVEINPVVTDVTVQEMEELVKDVDLILDATDNFETRLLINDISQKYNIPWIYGGCVGSYGVTYTIV
PGKTPCFRCLMEHPASGATCDTAGIIQPAVQLVVAHQITEALKILVEDFEALRETMLSFDLWNNQQMAFKVNRQKKDTCL
SCGRLRTYPSLTFEAQTKTEVLCGRNTVQIRPGVRNEFNLEEIKKRLQRSVDVKATPYLVSFLVEEFRFVLFTDGRAFIH
GTNDINVAKRLYTRYIG
>Mature_337_residues
MQERYSRQILFSGVGEEGQRKIREKHVLIIGAGALGAANAEAIVRAGVGKITIADRDYVEWSNLQRQQLYTEEDAKQYKP
KAVAAAEYLKAINSEVEINPVVTDVTVQEMEELVKDVDLILDATDNFETRLLINDISQKYNIPWIYGGCVGSYGVTYTIV
PGKTPCFRCLMEHPASGATCDTAGIIQPAVQLVVAHQITEALKILVEDFEALRETMLSFDLWNNQQMAFKVNRQKKDTCL
SCGRLRTYPSLTFEAQTKTEVLCGRNTVQIRPGVRNEFNLEEIKKRLQRSVDVKATPYLVSFLVEEFRFVLFTDGRAFIH
GTNDINVAKRLYTRYIG

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=267, Percent_Identity=30.7116104868914, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI38045948, Length=170, Percent_Identity=30, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI150417996, Length=171, Percent_Identity=28.6549707602339, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=32.9218106995885, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=33.8709677419355, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17540406, Length=265, Percent_Identity=32.4528301886792, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI193203301, Length=155, Percent_Identity=29.0322580645161, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17557754, Length=191, Percent_Identity=28.7958115183246, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=298, Percent_Identity=29.5302013422819, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320598, Length=174, Percent_Identity=33.3333333333333, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325323, Length=155, Percent_Identity=32.9032258064516, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322639, Length=186, Percent_Identity=29.5698924731183, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325438, Length=153, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24582879, Length=305, Percent_Identity=26.5573770491803, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28573937, Length=179, Percent_Identity=29.0502793296089, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24641311, Length=163, Percent_Identity=34.3558282208589, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 38072; Mature: 38072

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERYSRQILFSGVGEEGQRKIREKHVLIIGAGALGAANAEAIVRAGVGKITIADRDYVE
CCCHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQYKPKAVAAAEYLKAINSEVEINPVVTDVTVQEMEELVKDVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEECCEEECCCHHHHHHHHHHHHHE
LDATDNFETRLLINDISQKYNIPWIYGGCVGSYGVTYTIVPGKTPCFRCLMEHPASGATC
EECCCCCCEEEEEEHHHHHCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQLVVAHQITEALKILVEDFEALRETMLSFDLWNNQQMAFKVNRQKKDTCL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
SCGRLRTYPSLTFEAQTKTEVLCGRNTVQIRPGVRNEFNLEEIKKRLQRSVDVKATPYLV
HCCCCCCCCCEEEECCCCHHHEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
SFLVEEFRFVLFTDGRAFIHGTNDINVAKRLYTRYIG
HHHHHHCEEEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQERYSRQILFSGVGEEGQRKIREKHVLIIGAGALGAANAEAIVRAGVGKITIADRDYVE
CCCHHHHHHHHCCCCHHHHHHHHHCEEEEEECCCCCCCCHHHHHHCCCCEEEECCCCCHH
WSNLQRQQLYTEEDAKQYKPKAVAAAEYLKAINSEVEINPVVTDVTVQEMEELVKDVDLI
HHCCHHHHHHCHHHHHHCCCHHHHHHHHHHHHCCCEEECCEEECCCHHHHHHHHHHHHHE
LDATDNFETRLLINDISQKYNIPWIYGGCVGSYGVTYTIVPGKTPCFRCLMEHPASGATC
EECCCCCCEEEEEEHHHHHCCCCEEECCCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCC
DTAGIIQPAVQLVVAHQITEALKILVEDFEALRETMLSFDLWNNQQMAFKVNRQKKDTCL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHH
SCGRLRTYPSLTFEAQTKTEVLCGRNTVQIRPGVRNEFNLEEIKKRLQRSVDVKATPYLV
HCCCCCCCCCEEEECCCCHHHEECCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCHHHH
SFLVEEFRFVLFTDGRAFIHGTNDINVAKRLYTRYIG
HHHHHHCEEEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]