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The map label for this gene is iscS2 [H]

Identifier: 49187540

GI number: 49187540

Start: 4452201

End: 4453343

Strand: Reverse

Name: iscS2 [H]

Synonym: BAS4546

Alternate gene names: 49187540

Gene position: 4453343-4452201 (Counterclockwise)

Preceding gene: 49187541

Following gene: 49187539

Centisome position: 85.17

GC content: 40.33

Gene sequence:

>1143_bases
ATGATCTATTTTGATAATAGTGCGACGACAAAGCCATATCCAGAAGCTCTTCAATCATACGTAACGGTTGCGGGGAAATA
TTTTGGGAATCCTTCTTCTATTCATTCGCTTGGAGGAGAGGCAGAGCGTCTATTAACACAATCAAGAACAATTGCAGCGC
AGCTTCTTCACGTTAAACCTTCTGAAATTATTTTCACATCAGGTGGAACGGAAGGGAATAACCTTGCGATTAAAGGAATA
GCGATGAGGAATCGTTCGCGCGGTAAACATATCATTACAACGAATATTGAACACGCGTCTGTGTTTGAAGCATATAAACA
ATTAGAAGATCTCGGTTTTGATGTAACATATTTACCGGTTAATGAGCATGGTGTTGTATCGGTAGAGGATGTAAAGCGAG
CGCTTCGTGAAGATACGATTCTTGTGTCAATTATTCACGTGAACAACGAGACTGGAGCAATTCAGCCCGTTGCTGAAATT
GGAACGTTATTATCGAATTATCCGAAAATAAGATTCCATGTGGACCATGTACAAGGGATAGGAAAAGTACCGCTTAATTT
ATACGCTTCTCATATTGATCTTTGCTCAATATCTGGACATAAATTCCACAGTGTAAAAGGAACAGGTCTTCTTTACGTAC
GTGATGGCGTAAGGTTAGATCCAATTTTATCAGGTGGCCAACAAGAACTGAAGTATCGCTCTGGTACAGAGAACTTACCT
GGCATTGTAGCGATGGTGAAAGCACTTCGCATGACAATGGAACAAGTGAAAGAAAAGGTAGCTCATTTGCAAAGTTTACA
AGCAGAGCTTGTCCGTTTCTTTAAAGAAATGGAGGATGTAACAATCAATACGTCGCTTGCATATGCAGCACCGCACATTT
TAAATGTATCATTTGTTGGTTTAAAACCAGAAGTGGTCGTTCATGCTTTAGAAGAGCACGGTGTATATGTATCAACGAAA
TCTGCTTGTTCTTCAAAAGCAAATGAAGTGAGCAGAGTGTTAGTGTCAATGGGAGTACCGCATGCCGCTGCTGCAAGTGC
TATTCGCATTAGTTTGGCGCCAGAAAACACGATGGAAGAAGTAAAGCAATTTGAAGGTATTGTGAAAGAGACGATGCCAA
AATTATATGAAGTGATGAGGTAA

Upstream 100 bases:

>100_bases
TTCGTTTCAAAATATAACGGAACCTTTGAGTAGTCTTAGCGTAATGACACGTATTTGTGATATGATTATATGATGTGACA
GCGCTGAAAGGGGAAGAACG

Downstream 100 bases:

>100_bases
AGGAATATGATGACATATGAATATATTTTAGTTCGTTATGGAGAGATGACGACTAAAGGTAAGAACCGTTCTAAATTTGT
AAGCACATTAAAAGATAACG

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIYFDNSATTKPYPEALQSYVTVAGKYFGNPSSIHSLGGEAERLLTQSRTIAAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMRNRSRGKHIITTNIEHASVFEAYKQLEDLGFDVTYLPVNEHGVVSVEDVKRALREDTILVSIIHVNNETGAIQPVAEI
GTLLSNYPKIRFHVDHVQGIGKVPLNLYASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPILSGGQQELKYRSGTENLP
GIVAMVKALRMTMEQVKEKVAHLQSLQAELVRFFKEMEDVTINTSLAYAAPHILNVSFVGLKPEVVVHALEEHGVYVSTK
SACSSKANEVSRVLVSMGVPHAAAASAIRISLAPENTMEEVKQFEGIVKETMPKLYEVMR

Sequences:

>Translated_380_residues
MIYFDNSATTKPYPEALQSYVTVAGKYFGNPSSIHSLGGEAERLLTQSRTIAAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMRNRSRGKHIITTNIEHASVFEAYKQLEDLGFDVTYLPVNEHGVVSVEDVKRALREDTILVSIIHVNNETGAIQPVAEI
GTLLSNYPKIRFHVDHVQGIGKVPLNLYASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPILSGGQQELKYRSGTENLP
GIVAMVKALRMTMEQVKEKVAHLQSLQAELVRFFKEMEDVTINTSLAYAAPHILNVSFVGLKPEVVVHALEEHGVYVSTK
SACSSKANEVSRVLVSMGVPHAAAASAIRISLAPENTMEEVKQFEGIVKETMPKLYEVMR
>Mature_380_residues
MIYFDNSATTKPYPEALQSYVTVAGKYFGNPSSIHSLGGEAERLLTQSRTIAAQLLHVKPSEIIFTSGGTEGNNLAIKGI
AMRNRSRGKHIITTNIEHASVFEAYKQLEDLGFDVTYLPVNEHGVVSVEDVKRALREDTILVSIIHVNNETGAIQPVAEI
GTLLSNYPKIRFHVDHVQGIGKVPLNLYASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPILSGGQQELKYRSGTENLP
GIVAMVKALRMTMEQVKEKVAHLQSLQAELVRFFKEMEDVTINTSLAYAAPHILNVSFVGLKPEVVVHALEEHGVYVSTK
SACSSKANEVSRVLVSMGVPHAAAASAIRISLAPENTMEEVKQFEGIVKETMPKLYEVMR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=364, Percent_Identity=39.8351648351648, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=31.2348668280872, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI48994898, Length=383, Percent_Identity=40.7310704960835, Blast_Score=264, Evalue=7e-72,
Organism=Escherichia coli, GI1787970, Length=357, Percent_Identity=25.4901960784314, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789175, Length=405, Percent_Identity=23.7037037037037, Blast_Score=76, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=376, Percent_Identity=38.563829787234, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=34.1538461538462, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=380, Percent_Identity=38.4210526315789, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI20129463, Length=364, Percent_Identity=39.2857142857143, Blast_Score=235, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41699; Mature: 41699

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKPYPEALQSYVTVAGKYFGNPSSIHSLGGEAERLLTQSRTIAAQLLHVKP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
SEIIFTSGGTEGNNLAIKGIAMRNRSRGKHIITTNIEHASVFEAYKQLEDLGFDVTYLPV
CEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
NEHGVVSVEDVKRALREDTILVSIIHVNNETGAIQPVAEIGTLLSNYPKIRFHVDHVQGI
CCCCCEEHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCC
GKVPLNLYASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPILSGGQQELKYRSGTENLP
CCCCHHHHHHHCCEEECCCCEEEEECCCEEEEEECCCEECHHHCCCHHHHHCCCCCCCCC
GIVAMVKALRMTMEQVKEKVAHLQSLQAELVRFFKEMEDVTINTSLAYAAPHILNVSFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHCCHHEEEEEEC
LKPEVVVHALEEHGVYVSTKSACSSKANEVSRVLVSMGVPHAAAASAIRISLAPENTMEE
CCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCHHHHH
VKQFEGIVKETMPKLYEVMR
HHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MIYFDNSATTKPYPEALQSYVTVAGKYFGNPSSIHSLGGEAERLLTQSRTIAAQLLHVKP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
SEIIFTSGGTEGNNLAIKGIAMRNRSRGKHIITTNIEHASVFEAYKQLEDLGFDVTYLPV
CEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEEC
NEHGVVSVEDVKRALREDTILVSIIHVNNETGAIQPVAEIGTLLSNYPKIRFHVDHVQGI
CCCCCEEHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEEHHHCCC
GKVPLNLYASHIDLCSISGHKFHSVKGTGLLYVRDGVRLDPILSGGQQELKYRSGTENLP
CCCCHHHHHHHCCEEECCCCEEEEECCCEEEEEECCCEECHHHCCCHHHHHCCCCCCCCC
GIVAMVKALRMTMEQVKEKVAHLQSLQAELVRFFKEMEDVTINTSLAYAAPHILNVSFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCHHHHCCHHEEEEEEC
LKPEVVVHALEEHGVYVSTKSACSSKANEVSRVLVSMGVPHAAAASAIRISLAPENTMEE
CCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHCCCCHHHCCEEEEEECCCHHHHH
VKQFEGIVKETMPKLYEVMR
HHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]