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The map label for this gene is thrS [H]

Identifier: 49187467

GI number: 49187467

Start: 4382221

End: 4384158

Strand: Reverse

Name: thrS [H]

Synonym: BAS4472

Alternate gene names: 49187467

Gene position: 4384158-4382221 (Counterclockwise)

Preceding gene: 49187468

Following gene: 49187466

Centisome position: 83.85

GC content: 38.6

Gene sequence:

>1938_bases
ATGGCAGATGTAGTTAAAATTACTTTCCCTGATGGAGCTGTGAAGGAGTTTCCAAAAGGCGTAACAACTGAAGAAATCGC
AGCTTCTATTAGCCCAGGCTTAAAGAAAAAAGCTGTGGCTGGAAAATTAAATGATGAGATGATTGATCTTGTTACACCAA
TCGAAGAAGATGGTGCAGTTTCTATCATTACATTAGATTCTGAAGATGGCTTATATATTTTACGCCACTCAACAGCTCAC
CTTTTAGCGCAAGCGTTAAAACGTTTATATAAAGATGTTAAGGTTGAGCTTGGTATTGGTCCAGTAATTGAAAATGGCTT
CTACTACGATATTGATATGGAAGAAGCAATTACAGTTGAAGACTTCAAGAAAATCGAAAAAGAAATGCAAAAGATTGTGA
ACGAGAACTTAGAAATCGTTCGTCATGAAGTACCACGTGCAGAAGCAATTCGTCGCTTTGAAGAAATCGGCGATGAGTTA
AAATTAGATTTAATTAATGATCTTCCAGAAGATGCAGTTATTTCAATTTATGAGCAAGGCGAATTCTTCGACCTTTGCCG
TGGTGTTCACCTTCCATCTACAGGAAAAATTAAAGTATTTAAATTATTAAGCGTTGCGGGTGCTTACTGGCGTGGCGATA
GCAATAATAAAATGCTACAACGTATTTACGGTACTGCATTTGTTAAGAAAGCAGAATTAGATGAGCACTTACGTATGCTT
GAAGAAGCGAAAGAGCGCGATCACCGTAAATTAGGTAAAGAATTAAAACTATTTACTAATAGCCAAAAAGTAGGACAAGG
TTTACCACTTTGGTTACCAAAAGGTGCAACAATCCGCCGCATTATCGAGCGTTACATCGTTGATAAAGAAGCAAGCTTAG
GCTATGATCACGTATATACTCCAGTACTAGGAAGCAGAGAGCTTTATGAAACTTCTGGTCACTGGAATCACTACCGTGAT
GGTATGTTCCCATCAATGGAAATGGATAATGAAGAGTTAGTTCTTCGTCCAATGAACTGCCCTCACCACATGATGGTTTA
TAAAAACGATATTCACAGCTACCGTGAATTACCAATCCGTATTGCGGAACTTGGAACAATGCACCGCTATGAAATGTCAG
GTGCGTTATCTGGGTTACAACGTGTACGCGGAATGACTTTAAACGATGCGCACATTTTCGTTCGCCCAGATCAAATTAAA
GAAGAGTTAAAACGTGTTGTAAACTTAACTCTAGAAGTGTACAAAGATTTCGGTTTAGAGAACTACTCATTCCGTCTATC
TTATCGTGACCCAGAAGATACTAAAAAGTACTATGCTGATGATGAGATGTGGGAAAAAGCACAAGGTATGTTAAAAGAAG
CTATGGATGAAATGGGTCTTGATTACTACGAAGCTGAAGGTGAAGCGGCATTCTACGGTCCAAAACTTGACGTTCAAGTT
CGTACTGCTCTTGGAAAAGACGAAACACTTTCAACTGTACAATTAGACTTCTTACTTCCAGAACGCTTTGAACTATCTTA
CGTTGGTGAAGACGGTAAACAACACCGTCCAGTTGTAATTCACCGTGGTGTTGTATCAACTATGGAACGTTTCGTAGCAT
TCTTAATTGAAGAATACAAAGGTGCATTCCCAACTTGGTTAGCTCCAGTTCAAGTACAAGTAATTCCAGTTTCTCCGCAA
GTACATTTAGACTATGCGAAGAAAGTACAAGACGAATTGCGCCGTGCTGGTATCCGTGTTGAATTAGATACTCGTGAAGA
GAAAATTGGTTACAAAATCCGTGAAGCACAAATGCAAAAAATTCCGTACATGCTTGTAGTAGGTGACAATGAAGTAACTG
AAAACGGCGTAAACGTACGTAAATACGGTGAACAAAAATCAGAAACAATCGCATTAGATGCTTTTGTTGATATGATTAAA
GTAGAAGGAAAACGATAA

Upstream 100 bases:

>100_bases
ATAAGGTTAATTGGAACAAGGGTGGAACCACGAATTCAACACTCGTCCCTTTTTACGGGATGAGTGTTTTTTATTTTGAG
AAAAAGAGGAGTGACCAGTG

Downstream 100 bases:

>100_bases
GAAAAAGCCGCGGTATACCGCGGCTTTTCTGTGTAATATGAGTAAGAAAAATTAGAAAAAAAAACCATGTAACACTTATC
GTAAAAAAGTATTGCAAGTA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 645; Mature: 644

Protein sequence:

>645_residues
MADVVKITFPDGAVKEFPKGVTTEEIAASISPGLKKKAVAGKLNDEMIDLVTPIEEDGAVSIITLDSEDGLYILRHSTAH
LLAQALKRLYKDVKVELGIGPVIENGFYYDIDMEEAITVEDFKKIEKEMQKIVNENLEIVRHEVPRAEAIRRFEEIGDEL
KLDLINDLPEDAVISIYEQGEFFDLCRGVHLPSTGKIKVFKLLSVAGAYWRGDSNNKMLQRIYGTAFVKKAELDEHLRML
EEAKERDHRKLGKELKLFTNSQKVGQGLPLWLPKGATIRRIIERYIVDKEASLGYDHVYTPVLGSRELYETSGHWNHYRD
GMFPSMEMDNEELVLRPMNCPHHMMVYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIK
EELKRVVNLTLEVYKDFGLENYSFRLSYRDPEDTKKYYADDEMWEKAQGMLKEAMDEMGLDYYEAEGEAAFYGPKLDVQV
RTALGKDETLSTVQLDFLLPERFELSYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVQVIPVSPQ
VHLDYAKKVQDELRRAGIRVELDTREEKIGYKIREAQMQKIPYMLVVGDNEVTENGVNVRKYGEQKSETIALDAFVDMIK
VEGKR

Sequences:

>Translated_645_residues
MADVVKITFPDGAVKEFPKGVTTEEIAASISPGLKKKAVAGKLNDEMIDLVTPIEEDGAVSIITLDSEDGLYILRHSTAH
LLAQALKRLYKDVKVELGIGPVIENGFYYDIDMEEAITVEDFKKIEKEMQKIVNENLEIVRHEVPRAEAIRRFEEIGDEL
KLDLINDLPEDAVISIYEQGEFFDLCRGVHLPSTGKIKVFKLLSVAGAYWRGDSNNKMLQRIYGTAFVKKAELDEHLRML
EEAKERDHRKLGKELKLFTNSQKVGQGLPLWLPKGATIRRIIERYIVDKEASLGYDHVYTPVLGSRELYETSGHWNHYRD
GMFPSMEMDNEELVLRPMNCPHHMMVYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIK
EELKRVVNLTLEVYKDFGLENYSFRLSYRDPEDTKKYYADDEMWEKAQGMLKEAMDEMGLDYYEAEGEAAFYGPKLDVQV
RTALGKDETLSTVQLDFLLPERFELSYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVQVIPVSPQ
VHLDYAKKVQDELRRAGIRVELDTREEKIGYKIREAQMQKIPYMLVVGDNEVTENGVNVRKYGEQKSETIALDAFVDMIK
VEGKR
>Mature_644_residues
ADVVKITFPDGAVKEFPKGVTTEEIAASISPGLKKKAVAGKLNDEMIDLVTPIEEDGAVSIITLDSEDGLYILRHSTAHL
LAQALKRLYKDVKVELGIGPVIENGFYYDIDMEEAITVEDFKKIEKEMQKIVNENLEIVRHEVPRAEAIRRFEEIGDELK
LDLINDLPEDAVISIYEQGEFFDLCRGVHLPSTGKIKVFKLLSVAGAYWRGDSNNKMLQRIYGTAFVKKAELDEHLRMLE
EAKERDHRKLGKELKLFTNSQKVGQGLPLWLPKGATIRRIIERYIVDKEASLGYDHVYTPVLGSRELYETSGHWNHYRDG
MFPSMEMDNEELVLRPMNCPHHMMVYKNDIHSYRELPIRIAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKE
ELKRVVNLTLEVYKDFGLENYSFRLSYRDPEDTKKYYADDEMWEKAQGMLKEAMDEMGLDYYEAEGEAAFYGPKLDVQVR
TALGKDETLSTVQLDFLLPERFELSYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEYKGAFPTWLAPVQVQVIPVSPQV
HLDYAKKVQDELRRAGIRVELDTREEKIGYKIREAQMQKIPYMLVVGDNEVTENGVNVRKYGEQKSETIALDAFVDMIKV
EGKR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=636, Percent_Identity=38.0503144654088, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI38570111, Length=643, Percent_Identity=36.0808709175739, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI20070344, Length=637, Percent_Identity=35.4788069073783, Blast_Score=370, Evalue=1e-102,
Organism=Escherichia coli, GI1788013, Length=615, Percent_Identity=43.5772357723577, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71984192, Length=646, Percent_Identity=37.4613003095975, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71984184, Length=646, Percent_Identity=37.4613003095975, Blast_Score=434, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322112, Length=636, Percent_Identity=35.8490566037736, Blast_Score=412, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=36.4795918367347, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24583839, Length=619, Percent_Identity=38.4491114701131, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24583842, Length=619, Percent_Identity=38.4491114701131, Blast_Score=456, Evalue=1e-128,
Organism=Drosophila melanogaster, GI28574141, Length=619, Percent_Identity=38.4491114701131, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74057; Mature: 73926

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADVVKITFPDGAVKEFPKGVTTEEIAASISPGLKKKAVAGKLNDEMIDLVTPIEEDGAV
CCCEEEEECCCCHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCE
SIITLDSEDGLYILRHSTAHLLAQALKRLYKDVKVELGIGPVIENGFYYDIDMEEAITVE
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCEEEECCCCEECCCEEEEECCCCCCCHH
DFKKIEKEMQKIVNENLEIVRHEVPRAEAIRRFEEIGDELKLDLINDLPEDAVISIYEQG
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCC
EFFDLCRGVHLPSTGKIKVFKLLSVAGAYWRGDSNNKMLQRIYGTAFVKKAELDEHLRML
CHHHHHCCCCCCCCCCEEHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKERDHRKLGKELKLFTNSQKVGQGLPLWLPKGATIRRIIERYIVDKEASLGYDHVYT
HHHHHHHHHHHHHHHEEEECCHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCEEC
PVLGSRELYETSGHWNHYRDGMFPSMEMDNEELVLRPMNCPHHMMVYKNDIHSYRELPIR
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHCCEE
IAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEELKRVVNLTLEVYKDFGLE
EEHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
NYSFRLSYRDPEDTKKYYADDEMWEKAQGMLKEAMDEMGLDYYEAEGEAAFYGPKLDVQV
CEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCEEE
RTALGKDETLSTVQLDFLLPERFELSYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEYK
EECCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHC
GAFPTWLAPVQVQVIPVSPQVHLDYAKKVQDELRRAGIRVELDTREEKIGYKIREAQMQK
CCCCCEECCEEEEEEECCCCEEHHHHHHHHHHHHHCCCEEEECCCHHHHCCHHHHHHHHH
IPYMLVVGDNEVTENGVNVRKYGEQKSETIALDAFVDMIKVEGKR
CCEEEEECCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHCCCCC
>Mature Secondary Structure 
ADVVKITFPDGAVKEFPKGVTTEEIAASISPGLKKKAVAGKLNDEMIDLVTPIEEDGAV
CCEEEEECCCCHHHHCCCCCCHHHHHHHCCCCCHHHHHHCCCCCHHHHHHCCCCCCCCE
SIITLDSEDGLYILRHSTAHLLAQALKRLYKDVKVELGIGPVIENGFYYDIDMEEAITVE
EEEEEECCCCEEEEECCHHHHHHHHHHHHHHHCEEEECCCCEECCCEEEEECCCCCCCHH
DFKKIEKEMQKIVNENLEIVRHEVPRAEAIRRFEEIGDELKLDLINDLPEDAVISIYEQG
HHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCC
EFFDLCRGVHLPSTGKIKVFKLLSVAGAYWRGDSNNKMLQRIYGTAFVKKAELDEHLRML
CHHHHHCCCCCCCCCCEEHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKERDHRKLGKELKLFTNSQKVGQGLPLWLPKGATIRRIIERYIVDKEASLGYDHVYT
HHHHHHHHHHHHHHHEEEECCHHHCCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCEEC
PVLGSRELYETSGHWNHYRDGMFPSMEMDNEELVLRPMNCPHHMMVYKNDIHSYRELPIR
CCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEEECHHHHHHHCCEE
IAELGTMHRYEMSGALSGLQRVRGMTLNDAHIFVRPDQIKEELKRVVNLTLEVYKDFGLE
EEHHCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
NYSFRLSYRDPEDTKKYYADDEMWEKAQGMLKEAMDEMGLDYYEAEGEAAFYGPKLDVQV
CEEEEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEECCCCCEEE
RTALGKDETLSTVQLDFLLPERFELSYVGEDGKQHRPVVIHRGVVSTMERFVAFLIEEYK
EECCCCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCEEEECHHHHHHHHHHHHHHHHHC
GAFPTWLAPVQVQVIPVSPQVHLDYAKKVQDELRRAGIRVELDTREEKIGYKIREAQMQK
CCCCCEECCEEEEEEECCCCEEHHHHHHHHHHHHHCCCEEEECCCHHHHCCHHHHHHHHH
IPYMLVVGDNEVTENGVNVRKYGEQKSETIALDAFVDMIKVEGKR
CCEEEEECCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA