Click here to switch to the map view.

The map label for this gene is tig [H]

Identifier: 49187365

GI number: 49187365

Start: 4283140

End: 4284417

Strand: Reverse

Name: tig [H]

Synonym: BAS4370

Alternate gene names: 49187365

Gene position: 4284417-4283140 (Counterclockwise)

Preceding gene: 49187366

Following gene: 49187364

Centisome position: 81.94

GC content: 39.75

Gene sequence:

>1278_bases
ATGGCTGCAAAATGGGAAAAATTAGAAGGTAACGTAGGCGTTTTAACAATCGAAGTTGATGCTAAAGAAGTAAACAACTC
TATCGACGCTGCGTTCAAAAAAGTAGTAAAAACAATCAACGTACCAGGTTTCCGTAAAGGAAAAATGCCTCGTCCGTTAT
TCGAACAACGCTTTGGTATCGAATCTTTATACCAAGATGCTTTAGATATCATCTTACCAAAAGCATACGGTGAAGCGATC
GATGAAGCTGGTATCTTCCCAGTTGCTCATCCTGAAATCGACATCGAGAAGTTCGAAAAAAATGCTAACCTTATCTTCAC
TGCAAAAGTTACAGTGAAACCTGAAGTTAAATTAGGTGAGTACAAAGGTTTAGCAGTAGAAAAAGTTGAAACAACTGTAA
CTGACGAAGATGTAGAGAACGAATTAAAATCTTTACAAGAGCGTCAAGCTGAACTAGTTGTTAAAGAAGAAGGAACTGTT
GAAAACGGTGATACAGCTGTAATCGACTTCGAAGGTTTCGTTGATGGCGAAGCATTTGAAGGCGGAAAAGGCGAAAACTA
CTCTCTAGCAATCGGTTCTGGTACATTCATCCCAGGTTTCGAAGAGCAAGTAATTGGTCTTAAATCTGGTGAGTCTAAAG
ACGTTGAAGTATCATTCCCAGAAGAGTACCATGCTGCTGAATTAGCTGGCAAACCAGCAACATTCAAAGTAACAGTTCAC
GAAATCAAAACAAAAGAACTTCCTGAGTTAAACGACGAGTTCGCTAAAGAAGCTGACGAAGCGGTTGCAACTCTTGATGA
ATTAAAAGCAAAACTTCGCACAAACTTAGAAGAAGGCAAAAAGCACGAAGCTGAGCACAAAGTACGTGATGAAGTAGTAG
AATTAGCTGCTGCTAACGCTGAAATCGACATTCCAGAAGCTATGATCGACACTGAGTTAGATCGTATGGTTCGTGAATTC
GAGCAACGTTTAAGCCAACAAGGTATGAACCTTGAGCTTTACTACCAATTCACAGGTACTGATGCTGACAAGTTAAAAGA
GCAAATGAAAGAAGACGCTCAAAAACGCGTAAGAATCAACCTTGTTCTTGAAGCTATCATTGAAGCTGAAAACATCGAAG
TTACTGAAGAAGAAGTAACTGCAGAAGTTGAAAAAATGGCTGAAATGTACGGTATGCCAGTAGACGCTATCAAGCAAGCT
CTTGGAAGCGTAGACGCTTTAGCTGAAGATCTTAAAGTTCGTAAAGCTGTAGACTTCTTAGTAGAAAACGCTGCATAA

Upstream 100 bases:

>100_bases
AGGATTATTTGTACTTTTGTTATTTCAAAAAAAGAGATAATGAAGATCTTAATCTATAGAGGTATGTGCATACATTATTA
TAGTTGGAGGGAAAGAATAA

Downstream 100 bases:

>100_bases
TAAAATAACAAGGCGCGATTTTAATCGTGCCTTGTTTTATAAATATAGAAATATATTTATAAAACTTTTCGATGAAGAAG
GAAAACTTCCTTAATAAGTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAAKWEKLEGNVGVLTIEVDAKEVNNSIDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGIESLYQDALDIILPKAYGEAI
DEAGIFPVAHPEIDIEKFEKNANLIFTAKVTVKPEVKLGEYKGLAVEKVETTVTDEDVENELKSLQERQAELVVKEEGTV
ENGDTAVIDFEGFVDGEAFEGGKGENYSLAIGSGTFIPGFEEQVIGLKSGESKDVEVSFPEEYHAAELAGKPATFKVTVH
EIKTKELPELNDEFAKEADEAVATLDELKAKLRTNLEEGKKHEAEHKVRDEVVELAAANAEIDIPEAMIDTELDRMVREF
EQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIEAENIEVTEEEVTAEVEKMAEMYGMPVDAIKQA
LGSVDALAEDLKVRKAVDFLVENAA

Sequences:

>Translated_425_residues
MAAKWEKLEGNVGVLTIEVDAKEVNNSIDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGIESLYQDALDIILPKAYGEAI
DEAGIFPVAHPEIDIEKFEKNANLIFTAKVTVKPEVKLGEYKGLAVEKVETTVTDEDVENELKSLQERQAELVVKEEGTV
ENGDTAVIDFEGFVDGEAFEGGKGENYSLAIGSGTFIPGFEEQVIGLKSGESKDVEVSFPEEYHAAELAGKPATFKVTVH
EIKTKELPELNDEFAKEADEAVATLDELKAKLRTNLEEGKKHEAEHKVRDEVVELAAANAEIDIPEAMIDTELDRMVREF
EQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIEAENIEVTEEEVTAEVEKMAEMYGMPVDAIKQA
LGSVDALAEDLKVRKAVDFLVENAA
>Mature_424_residues
AAKWEKLEGNVGVLTIEVDAKEVNNSIDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGIESLYQDALDIILPKAYGEAID
EAGIFPVAHPEIDIEKFEKNANLIFTAKVTVKPEVKLGEYKGLAVEKVETTVTDEDVENELKSLQERQAELVVKEEGTVE
NGDTAVIDFEGFVDGEAFEGGKGENYSLAIGSGTFIPGFEEQVIGLKSGESKDVEVSFPEEYHAAELAGKPATFKVTVHE
IKTKELPELNDEFAKEADEAVATLDELKAKLRTNLEEGKKHEAEHKVRDEVVELAAANAEIDIPEAMIDTELDRMVREFE
QRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRINLVLEAIIEAENIEVTEEEVTAEVEKMAEMYGMPVDAIKQAL
GSVDALAEDLKVRKAVDFLVENAA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=400, Percent_Identity=33.75, Blast_Score=214, Evalue=1e-56,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 47215; Mature: 47084

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKWEKLEGNVGVLTIEVDAKEVNNSIDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGI
CCCCCCCCCCCEEEEEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCH
ESLYQDALDIILPKAYGEAIDEAGIFPVAHPEIDIEKFEKNANLIFTAKVTVKPEVKLGE
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEECCCCEECC
YKGLAVEKVETTVTDEDVENELKSLQERQAELVVKEEGTVENGDTAVIDFEGFVDGEAFE
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYSLAIGSGTFIPGFEEQVIGLKSGESKDVEVSFPEEYHAAELAGKPATFKVTVH
CCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEEE
EIKTKELPELNDEFAKEADEAVATLDELKAKLRTNLEEGKKHEAEHKVRDEVVELAAANA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
EIDIPEAMIDTELDRMVREFEQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRIN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLEAIIEAENIEVTEEEVTAEVEKMAEMYGMPVDAIKQALGSVDALAEDLKVRKAVDFL
HHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENAA
HHCCC
>Mature Secondary Structure 
AAKWEKLEGNVGVLTIEVDAKEVNNSIDAAFKKVVKTINVPGFRKGKMPRPLFEQRFGI
CCCCCCCCCCEEEEEEEECHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCH
ESLYQDALDIILPKAYGEAIDEAGIFPVAHPEIDIEKFEKNANLIFTAKVTVKPEVKLGE
HHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCEEEEEEEEECCCCEECC
YKGLAVEKVETTVTDEDVENELKSLQERQAELVVKEEGTVENGDTAVIDFEGFVDGEAFE
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEEEECCCCCCCCCC
GGKGENYSLAIGSGTFIPGFEEQVIGLKSGESKDVEVSFPEEYHAAELAGKPATFKVTVH
CCCCCCEEEEECCCCCCCCCHHHEEECCCCCCCCEEEECCHHHHHHHHCCCCCEEEEEEE
EIKTKELPELNDEFAKEADEAVATLDELKAKLRTNLEEGKKHEAEHKVRDEVVELAAANA
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
EIDIPEAMIDTELDRMVREFEQRLSQQGMNLELYYQFTGTDADKLKEQMKEDAQKRVRIN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHH
LVLEAIIEAENIEVTEEEVTAEVEKMAEMYGMPVDAIKQALGSVDALAEDLKVRKAVDFL
HHHHHHHHHCCCEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VENAA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA