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The map label for this gene is hemB [H]

Identifier: 49187354

GI number: 49187354

Start: 4270518

End: 4271507

Strand: Reverse

Name: hemB [H]

Synonym: BAS4359

Alternate gene names: 49187354

Gene position: 4271507-4270518 (Counterclockwise)

Preceding gene: 49187355

Following gene: 49187353

Centisome position: 81.69

GC content: 38.69

Gene sequence:

>990_bases
ATGAACTCTTTACAATTTAATCGTCATCGTCGCTTAAGACAAAGCGGGGGCATGCGTGCGCTTGTGCGTGAAACGTTTTT
ACATACGGAAGATTTTATTTACCCTATTTTTGTATTAGAAGGTGAAAATGTTCGTAATGAAGTACCTTCTATGCCAGGCG
TATATCAAATGTCTTTAGATTTATTGCAAGCTGAAATGCAAGAAGTTGTTGATTTAGGTATTCGTTCTGTTATTGTATTT
GGTTTACCTGCTGAAAAAGATGAAGTTGGATCATCAGCATATTGTGATCATGGAATTGTGCAACGCGCGATTCAGCAAAT
TAAAGGTGAATTCCCCGATCTAGTAGTAGTTGCGGATACATGTTTATGTCAATTTACAAGCCATGGTCATTGCGGTGTAA
TTGAAGATGGTATTATTTTAAATGACGAGTCTCTTGCAGTTCTTGCAAAAACAGCTGTAAGCCAAGCGAAAGCAGGAGCG
GACATTATTGCGCCATCAAACATGATGGACGGATTCGTAACAGCAATTCGCCACGCATTAGATGAAAATGGTTTTGGACA
TGTACCAGTTATGTCGTACGCTGTGAAATATTCATCAGCATTTTATGGACCATTCCGTGATGCGGCACACGGTGCACCGC
AATTTGGTGATCGTAAAACATATCAAATGGACCCAGCGAACCGCATGGAAGCATTCCGTGAAGCAGAATCAGATGTAATG
GAAGGGGCAGATTTCTTAATTGTAAAACCAGCTCTTTCTTATTTAGATATCGTTCGTGATGTGAAAAATAACTTTAATTT
ACCAGTCGTTGCTTATAACGTAAGCGGTGAATATTCAATGATTAAAGCGGCAGCGCAAAATGGTTGGATTAATGAAAAAG
AAGTTGTACTTGAAAAATTAATTAGTATGAAACGTGCAGGAGCAGATTTAATTATTACGTATCATGCAAAAGATGCAGCA
AGATGGTTACAAGAAGGAGGCGCTAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTCCGCATGTTATTATGCCGAAAGAGTACACTGTAGAAGCATTACTACAATGCGTTTGTGAATCTATAAAATAA
AAGAATGGGGATAGAAATCT

Downstream 100 bases:

>100_bases
TGAGAAAATTTGATAAGTCGATTGCAGCGTTTGAAGAGGCTCAAGATTTAATGCCTGGTGGCGTAAATAGCCCAGTTCGT
GCATTTAAGTCTGTCGGTAT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSSAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK

Sequences:

>Translated_329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSSAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK
>Mature_329_residues
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLDLLQAEMQEVVDLGIRSVIVF
GLPAEKDEVGSSAYCDHGIVQRAIQQIKGEFPDLVVVADTCLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGA
DIIAPSNMMDGFVTAIRHALDENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKLISMKRAGADLIITYHAKDAA
RWLQEGGAK

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=322, Percent_Identity=44.7204968944099, Blast_Score=278, Evalue=5e-75,
Organism=Escherichia coli, GI87081728, Length=315, Percent_Identity=50.7936507936508, Blast_Score=297, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6321398, Length=323, Percent_Identity=43.343653250774, Blast_Score=252, Evalue=5e-68,
Organism=Drosophila melanogaster, GI21358291, Length=309, Percent_Identity=40.1294498381877, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36340; Mature: 36340

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLD
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLQAEMQEVVDLGIRSVIVFGLPAEKDEVGSSAYCDHGIVQRAIQQIKGEFPDLVVVADT
HHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCHHCHHHHHHHHHHHHHCCCCCEEEEHHH
CLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGADIIAPSNMMDGFVTAIRHAL
HHHHHCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKL
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHH
ISMKRAGADLIITYHAKDAARWLQEGGAK
HHHHHCCCCEEEEEECHHHHHHHHHCCCC
>Mature Secondary Structure
MNSLQFNRHRRLRQSGGMRALVRETFLHTEDFIYPIFVLEGENVRNEVPSMPGVYQMSLD
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCHHHHCCCCCCHHHHHHH
LLQAEMQEVVDLGIRSVIVFGLPAEKDEVGSSAYCDHGIVQRAIQQIKGEFPDLVVVADT
HHHHHHHHHHHHHHHHEEEECCCCCHHHCCCCHHCHHHHHHHHHHHHHCCCCCEEEEHHH
CLCQFTSHGHCGVIEDGIILNDESLAVLAKTAVSQAKAGADIIAPSNMMDGFVTAIRHAL
HHHHHCCCCCCCEECCCEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHH
DENGFGHVPVMSYAVKYSSAFYGPFRDAAHGAPQFGDRKTYQMDPANRMEAFREAESDVM
CCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHH
EGADFLIVKPALSYLDIVRDVKNNFNLPVVAYNVSGEYSMIKAAAQNGWINEKEVVLEKL
CCCCEEEEHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHH
ISMKRAGADLIITYHAKDAARWLQEGGAK
HHHHHCCCCEEEEEECHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1672867; 9384377 [H]