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The map label for this gene is nifS [H]

Identifier: 49187323

GI number: 49187323

Start: 4239500

End: 4240639

Strand: Direct

Name: nifS [H]

Synonym: BAS4328

Alternate gene names: 49187323

Gene position: 4239500-4240639 (Clockwise)

Preceding gene: 49187317

Following gene: 49187324

Centisome position: 81.08

GC content: 37.54

Gene sequence:

>1140_bases
ATGATGATATATCTTGACTATGCAGCTACTACACCTATGAGTGTGGAAGCATTACAAACATATACGAAAGCAGCATCGCA
ATACTTCGGAAACGAACAAAGTTTACACGATATTGGAGGAACAGCCTCTTCTTTATTACAAGTTTGCAGAAAAACATTTG
CTGACATGATCGGTGGAAAGGAACAAGGGGTATTCTTCACGAGCGGCGGGTCAGAATCGAACTATCTTGCGATTCAATCC
CTTCTCAATGCCCAAAATAAGAAGCATATTATTACGACACCTATGGAACATGCATCCATTCGAAGTTATTTTCAATCTCT
AAAATCACAAGGCTATACGATTACTGAAATTCCTGTTGATAAAAGTGGACTCATTCGTTTAGTAGATTTAGAAACAGCTA
TTACAGAAGATACTGTTCTAGCAAGTATACAGCACGGAAACTCTGAAATCGGAACCGTTCAAAATATCGCAGAGATCGGG
GCACTTTTAAAAAAATATAACGTTTTATTTCATTCAGATTGTGTGCAAACATTTGGTAAACTTCCTATACATGTTTTTGA
GATGGGGATTGATAGTCTTTCTGTTTCAGCACATAAAATATACGGACCAAAGGGTGTTGGCGCTTGCTATATAAATCCGC
AAGTTCGCTGGACACAAGTATTTCCGGAAACTTCTCACGAAAAAGGTTTTCGCCCTGGCACAGTAAACGTCCCTGGCATC
GCATCTTTTTTAACAGCTGCTGAGAATATATTAAAAAATCAACAAGAAGAAAGTTTACGTTTTAAAGAGTTACGATCCTA
CTTTTTAGAGCAAATACAAACACTTCCGCTAGAAATTGAAGTAGAAGGTCATTCTACTTCTTGTTTACCACATATTATTG
GGGTTACAATAAAAGGAATAGAAGGTCAGTATACAATGCTAGAATGTAATCGCCGCGGTATTGCCATTTCCACGGGAAGT
GCTTGCCAAGTCGGTAAACAAGAACCTTCGAAAACAATGCTTGCAATTGGAAAAACGTATGAAGAGGCAAAACAATATGT
CCGTTTCTCTTTCGGACAACAAACAACGAAAGATCAAATTGATACTACTATTCATGCACTACACACAATTGGAAATCAAT
TTTATAGAGGTGTTAAATGA

Upstream 100 bases:

>100_bases
CATAGTTTTAAAATAATGACAAGAGTTTTTTTATAATTCTTATTTTCACTATCATTTAAAAATAAAAGAAATGTAACTCT
TACTAGAGAGAGGAACTTAC

Downstream 100 bases:

>100_bases
TAATGAAACAAAATGAACAAAAAAAGATTTTAGGTGAAGAAAGACGACAACTTATCCTTCAGTGGCTTCTAGCTGCAAAT
GAACCGTTATCTGGGAATGA

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK

Sequences:

>Translated_379_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK
>Mature_379_residues
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGKEQGVFFTSGGSESNYLAIQS
LLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVDKSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIG
ALLKKYNVLFHSDCVQTFGKLPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGI
ASFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGIEGQYTMLECNRRGIAISTGS
ACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQIDTTIHALHTIGNQFYRGVK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Seems to be required for NAD biosynthesis [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=368, Percent_Identity=35.8695652173913, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI156713448, Length=406, Percent_Identity=31.5270935960591, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=369, Percent_Identity=35.7723577235772, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1787970, Length=399, Percent_Identity=23.0576441102757, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=368, Percent_Identity=34.7826086956522, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17533177, Length=330, Percent_Identity=30.9090909090909, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319831, Length=370, Percent_Identity=35.4054054054054, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI20129463, Length=366, Percent_Identity=34.4262295081967, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41824; Mature: 41824

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
EQGVFFTSGGSESNYLAIQSLLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVD
CCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCC
KSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGALLKKYNVLFHSDCVQTFGK
CCCCEEEEECHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGI
CCEEEEECCCCHHCCCHHEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHH
ASFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECC
EGQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQI
CCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHH
DTTIHALHTIGNQFYRGVK
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MMIYLDYAATTPMSVEALQTYTKAASQYFGNEQSLHDIGGTASSLLQVCRKTFADMIGGK
CEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHHHCCC
EQGVFFTSGGSESNYLAIQSLLNAQNKKHIITTPMEHASIRSYFQSLKSQGYTITEIPVD
CCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCC
KSGLIRLVDLETAITEDTVLASIQHGNSEIGTVQNIAEIGALLKKYNVLFHSDCVQTFGK
CCCCEEEEECHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LPIHVFEMGIDSLSVSAHKIYGPKGVGACYINPQVRWTQVFPETSHEKGFRPGTVNVPGI
CCEEEEECCCCHHCCCHHEEECCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCHH
ASFLTAAENILKNQQEESLRFKELRSYFLEQIQTLPLEIEVEGHSTSCLPHIIGVTIKGI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCEEEECC
EGQYTMLECNRRGIAISTGSACQVGKQEPSKTMLAIGKTYEEAKQYVRFSFGQQTTKDQI
CCCEEEEEECCCCEEEECCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCHHHH
DTTIHALHTIGNQFYRGVK
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]