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The map label for this gene is mtnN

Identifier: 49187266

GI number: 49187266

Start: 4181840

End: 4182535

Strand: Reverse

Name: mtnN

Synonym: BAS4270

Alternate gene names: 49187266

Gene position: 4182535-4181840 (Counterclockwise)

Preceding gene: 49187267

Following gene: 49187265

Centisome position: 79.99

GC content: 36.64

Gene sequence:

>696_bases
TTGAGAATTGCTGTAATTGGAGCAATGGAAGAAGAAGTACGTATTTTACGTGACAAATTAGAACAAGCAGAAACAGAAAC
GGTTGCGGGTTGTGAATTTACGAAAGGGCAATTAGCAGGACATGAAGTAATCTTGTTAAAGTCTGGTATTGGTAAAGTAA
ATGCAGCGATGTCAACGACAATTTTATTAGAAAGATATAAGCCTGAAAAAGTAATCAATACTGGTTCAGCTGGTGGATTC
CATCATTCTCTAAATGTTGGAGATGTCGTTATTTCAACAGAAGTTCGTCACCATGACGTAGATGTAACAGCATTTAACTA
TGAATATGGTCAAGTACCAGGAATGCCGCCTGGATTCAAGGCTGATGAAGCATTAGTTGCATTAGCTGAGAAATGTATGC
AGGCAGAAGAAAATATTCAAGTTGTAAAAGGTATGATTGCAACAGGTGATTCATTTATGAGTGATCCGAATCGCGTTGCA
GCCATTCGTGATAAATTCGAAAATCTTTATGCAGTAGAAATGGAAGCAGCAGCTGTTGCACAAGTATGCCACCAATATGA
AGTTCCGTTTGTTATCATTCGCGCACTTTCTGATATTGCTGGTAAAGAATCAAATGTTTCATTTGATCAATTTTTAGATC
AAGCAGCTCTTCATTCTACAAACTTTATTGTAAAAGTATTAGAAGAGTTAAAGTAA

Upstream 100 bases:

>100_bases
AGACTATTTTTGAAAACAATGGCTTCCATGTAACGTTTACGAGATTAAATCATTTCGTGTGGGTAATGGAGGCAACTAAG
CAATAGAAAGAGGGATTAGA

Downstream 100 bases:

>100_bases
TGTAACCAAAAGGCAACTGTCTCATATAAAGAAAATACATACAGTTGCCTTTTCTTTATTGGCAAATAAGGGGGAGAACA
CGATGAATGTATATCGTGGA

Product: 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MRIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTTILLERYKPEKVINTGSAGGF
HHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFKADEALVALAEKCMQAEENIQVVKGMIATGDSFMSDPNRVA
AIRDKFENLYAVEMEAAAVAQVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKVLEELK

Sequences:

>Translated_231_residues
MRIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTTILLERYKPEKVINTGSAGGF
HHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFKADEALVALAEKCMQAEENIQVVKGMIATGDSFMSDPNRVA
AIRDKFENLYAVEMEAAAVAQVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKVLEELK
>Mature_231_residues
MRIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTTILLERYKPEKVINTGSAGGF
HHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFKADEALVALAEKCMQAEENIQVVKGMIATGDSFMSDPNRVA
AIRDKFENLYAVEMEAAAVAQVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKVLEELK

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily

Homologues:

Organism=Escherichia coli, GI1786354, Length=230, Percent_Identity=54.3478260869565, Blast_Score=233, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNN_BACAA (C3P964)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868663.1
- ProteinModelPortal:   C3P964
- SMR:   C3P964
- EnsemblBacteria:   EBBACT00000128343
- GeneID:   7851744
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4622
- GeneTree:   EBGT00050000000478
- ProtClustDB:   PRK05584
- HAMAP:   MF_01684
- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845
- PANTHER:   PTHR21234
- TIGRFAMs:   TIGR01704

Pfam domain/function: PF01048 PNP_UDP_1

EC number: =3.2.2.9

Molecular weight: Translated: 25255; Mature: 25255

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: ACT_SITE 12-12 BINDING 78-78 BINDING 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTT
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
ILLERYKPEKVINTGSAGGFHHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFK
HHHHHCCCHHHCCCCCCCCEEECCCCCCEEEEECCEECCCEEEEEECCCCCCCCCCCCCC
ADEALVALAEKCMQAEENIQVVKGMIATGDSFMSDPNRVAAIRDKFENLYAVEMEAAAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCEEEEHHHHHHHH
QVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKVLEELK
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRIAVIGAMEEEVRILRDKLEQAETETVAGCEFTKGQLAGHEVILLKSGIGKVNAAMSTT
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHH
ILLERYKPEKVINTGSAGGFHHSLNVGDVVISTEVRHHDVDVTAFNYEYGQVPGMPPGFK
HHHHHCCCHHHCCCCCCCCEEECCCCCCEEEEECCEECCCEEEEEECCCCCCCCCCCCCC
ADEALVALAEKCMQAEENIQVVKGMIATGDSFMSDPNRVAAIRDKFENLYAVEMEAAAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCEEEEHHHHHHHH
QVCHQYEVPFVIIRALSDIAGKESNVSFDQFLDQAALHSTNFIVKVLEELK
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA