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The map label for this gene is grpE [H]

Identifier: 49187210

GI number: 49187210

Start: 4129890

End: 4130456

Strand: Reverse

Name: grpE [H]

Synonym: BAS4214

Alternate gene names: 49187210

Gene position: 4130456-4129890 (Counterclockwise)

Preceding gene: 49187211

Following gene: 49187209

Centisome position: 79.0

GC content: 38.27

Gene sequence:

>567_bases
GTGGAAGAGCGTAACGAACAAGTGGTAGAAGAAGTAAAAGAAGCGCAAGTTGAAGAAGCTGTCACGCCAGAAAACAGTGA
AGAAACTGTAGAAGAAAAAAGTGAGGCTGCTCTTTTACAAGAAAAAGTAGATGAGCTACAAGCGAAACTAACGGAAACGG
AAGGTCGCACATTACGTCTACAAGCTGATTTTGAAAATTATAAGCGCCGTGTCCAAATGGATAAACAGGCTGCTGAAAAA
TATAGAGCACAAAGTCTAGTTTCAGATATTTTGCCAGCTCTTGATAATTTTGAAAGAGCAATGCAAGTGGAAGCAACTGA
TGAGCAAACGAAATCCCTGTTACAAGGTATGGAAATGGTGCATCGTCAATTGCTAGAAGCATTAAATAAAGAAGGTGTTG
AAGTGATTGAAGCTGTTGGTAAACAGTTTGATCCTAATGAACACCAAGCTATTATGCAAGTGGAAGACAGTGAATTTGAA
TCAAATGCGGTAGTTGAAGAATTCCAAAAAGGTTATAAACTAAAAGACCGTGTGATTCGCCCATCAATGGTAAAAGTAAA
TCAATAA

Upstream 100 bases:

>100_bases
TTCGCTCAAGTGATGAACTTTTCATTGCTGTGTGTAATATATAATGCTTAGGTAATTGTGTTTAACATGGTACCTAATAC
CTTTTAAGGAGGTGAATATT

Downstream 100 bases:

>100_bases
TTTACATAAAAGTAGGAGGATTCGCCATGAGTAAAATTATCGGTATTGACTTAGGTACAACAAACTCTTGTGTAGCTGTT
ATGGAAGGTGGAGAACCAAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFENYKRRVQMDKQAAEK
YRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFE
SNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Sequences:

>Translated_188_residues
MEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFENYKRRVQMDKQAAEK
YRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFE
SNAVVEEFQKGYKLKDRVIRPSMVKVNQ
>Mature_188_residues
MEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRLQADFENYKRRVQMDKQAAEK
YRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMVHRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFE
SNAVVEEFQKGYKLKDRVIRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=161, Percent_Identity=34.7826086956522, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1788967, Length=173, Percent_Identity=35.8381502890173, Blast_Score=108, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324806, Length=178, Percent_Identity=32.5842696629214, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24653432, Length=135, Percent_Identity=34.8148148148148, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21657; Mature: 21657

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRL
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
QADFENYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMV
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFESNAVVEEFQKGYKLKDRVIR
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCC
PSMVKVNQ
CCHHCCCC
>Mature Secondary Structure
MEERNEQVVEEVKEAQVEEAVTPENSEETVEEKSEAALLQEKVDELQAKLTETEGRTLRL
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
QADFENYKRRVQMDKQAAEKYRAQSLVSDILPALDNFERAMQVEATDEQTKSLLQGMEMV
EECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
HRQLLEALNKEGVEVIEAVGKQFDPNEHQAIMQVEDSEFESNAVVEEFQKGYKLKDRVIR
HHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHCC
PSMVKVNQ
CCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA