Click here to switch to the map view.

The map label for this gene is metB [H]

Identifier: 49187155

GI number: 49187155

Start: 4074987

End: 4076099

Strand: Direct

Name: metB [H]

Synonym: BAS4158

Alternate gene names: 49187155

Gene position: 4074987-4076099 (Clockwise)

Preceding gene: 49187148

Following gene: 49187156

Centisome position: 77.94

GC content: 39.44

Gene sequence:

>1113_bases
ATGTCAACTATCGAAACAAAACTAGCGCAAATCGGAAACCGGAGTGAAACTACAACAGGAACTGTTAATCCGCCTGTTTA
CTTTTCAACTGCTTATCGTCACGAAGGAATTGGTAAATCTACTGGATTTGACTATTCACGAACTGGAAATCCAACTCGCG
GCCTATTAGAACAGGCAATCGCAGACTTAGAATACGGCGAACAAGGTTATGCCTGTAGTTCCGGTATGGCAGCTGTGCTC
CTTGTCCTTTCCCTATTCCGCTCTGGAGACGAACTTATTGTATCCGAAGATTTATACGGGGGCACTTATCGATTATTTTC
TGAGCACGAAAAAAAGTGGAATGTTCGATGTAGATACGTAAATACACAATCTATTAAACAAATTGAGCAAGCTATCACAA
CTGAAACGAAAGCTATTTTCATAGAAACCCCGACAAATCCATTAATGCAAGTTACTGATATTGCCGCTGTCGCAACTGTA
GCAAAAAGACACGGACTACTTCTTATTGTAGACAACACATTCTACACGCCTTATATACAGCAGCCATTAACAGAAGGTGC
TGACATTGTACTTCATAGCGCAACAAAATATTTAGGAGGACATAACGATGTACTAAGCGGACTTGTCGTTGCAAAAGGAA
AGGAACTTTGTGAGGAAATCGCCCATTATCATAATGCATCAGGTGCGGTCTTAAGTCCATTTGACTCATGGCTATTAATC
CGTGGTATGAAAACGTTAGCGCTTCGCATGAGACAACATGAAAAAAATGCAAAAGCAGTTGTTGCTTATTTAAATGATGA
GGACGGGGTGACAGATGTATTTTATCCTGGAAGAGGCGGCATGATCTCATTTCGTCTTAAAGATGAAACATGGATTAATC
CATTCTTACAATCTTTATCCTTAATTACATTCGCCGAAAGTCTTGGTGGTGTTGAAAGTTTAATGACATATCCAGCAACA
CAAACACACGCTGATATTCCTGAAGAAATCAGAACAGCAAACGGTGTATGTAATCGTCTTCTTCGATTTTCTGTTGGCAT
TGAAAATAGTAACGATTTAATTCAAGACTTACAGCAAGCCATTAAACTTGTAAAAGAAGGTGTAAGAATATGA

Upstream 100 bases:

>100_bases
TGAGAAGAAGACTCTATTCAAACCGAAAGCCTTCTTCTTAGAAGGCTTTTTTTATTTTATATTCAACTACTGGTTCAATT
TAAAAAGGAGGAATTTTTAC

Downstream 100 bases:

>100_bases
GTTATTCTATAGATACACTCTTACTACACAACCAATATAAACATGATCCACAAACAGGAGCTGTTAACGTTCCCATCTAT
AACACATCAACATTCCACCA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-acetylhomoserine (thiol)-lyase; O-acetylhomoserine sulfhydrylase; OAH sulfhydrylase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSTIETKLAQIGNRSETTTGTVNPPVYFSTAYRHEGIGKSTGFDYSRTGNPTRGLLEQAIADLEYGEQGYACSSGMAAVL
LVLSLFRSGDELIVSEDLYGGTYRLFSEHEKKWNVRCRYVNTQSIKQIEQAITTETKAIFIETPTNPLMQVTDIAAVATV
AKRHGLLLIVDNTFYTPYIQQPLTEGADIVLHSATKYLGGHNDVLSGLVVAKGKELCEEIAHYHNASGAVLSPFDSWLLI
RGMKTLALRMRQHEKNAKAVVAYLNDEDGVTDVFYPGRGGMISFRLKDETWINPFLQSLSLITFAESLGGVESLMTYPAT
QTHADIPEEIRTANGVCNRLLRFSVGIENSNDLIQDLQQAIKLVKEGVRI

Sequences:

>Translated_370_residues
MSTIETKLAQIGNRSETTTGTVNPPVYFSTAYRHEGIGKSTGFDYSRTGNPTRGLLEQAIADLEYGEQGYACSSGMAAVL
LVLSLFRSGDELIVSEDLYGGTYRLFSEHEKKWNVRCRYVNTQSIKQIEQAITTETKAIFIETPTNPLMQVTDIAAVATV
AKRHGLLLIVDNTFYTPYIQQPLTEGADIVLHSATKYLGGHNDVLSGLVVAKGKELCEEIAHYHNASGAVLSPFDSWLLI
RGMKTLALRMRQHEKNAKAVVAYLNDEDGVTDVFYPGRGGMISFRLKDETWINPFLQSLSLITFAESLGGVESLMTYPAT
QTHADIPEEIRTANGVCNRLLRFSVGIENSNDLIQDLQQAIKLVKEGVRI
>Mature_369_residues
STIETKLAQIGNRSETTTGTVNPPVYFSTAYRHEGIGKSTGFDYSRTGNPTRGLLEQAIADLEYGEQGYACSSGMAAVLL
VLSLFRSGDELIVSEDLYGGTYRLFSEHEKKWNVRCRYVNTQSIKQIEQAITTETKAIFIETPTNPLMQVTDIAAVATVA
KRHGLLLIVDNTFYTPYIQQPLTEGADIVLHSATKYLGGHNDVLSGLVVAKGKELCEEIAHYHNASGAVLSPFDSWLLIR
GMKTLALRMRQHEKNAKAVVAYLNDEDGVTDVFYPGRGGMISFRLKDETWINPFLQSLSLITFAESLGGVESLMTYPATQ
THADIPEEIRTANGVCNRLLRFSVGIENSNDLIQDLQQAIKLVKEGVRI

Specific function: Catalyzes the transformation of O-succinylhomoserine into cystathionine, as well as O-acetylhomoserine and sulfide into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=376, Percent_Identity=41.4893617021277, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI299473759, Length=376, Percent_Identity=38.2978723404255, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI25453487, Length=375, Percent_Identity=35.7333333333333, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1790375, Length=366, Percent_Identity=41.2568306010929, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1789383, Length=391, Percent_Identity=26.3427109974425, Blast_Score=121, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17560056, Length=384, Percent_Identity=40.3645833333333, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI17538055, Length=362, Percent_Identity=41.7127071823204, Blast_Score=276, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71980708, Length=367, Percent_Identity=36.2397820163488, Blast_Score=209, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6319307, Length=368, Percent_Identity=42.3913043478261, Blast_Score=281, Evalue=1e-76,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=31.5, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=416, Percent_Identity=31.4903846153846, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321170, Length=277, Percent_Identity=33.2129963898917, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=391, Percent_Identity=27.8772378516624, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI19922554, Length=368, Percent_Identity=39.945652173913, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48; =2.5.1.49 [H]

Molecular weight: Translated: 40863; Mature: 40732

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00868 CYS_MET_METAB_PP ; PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIETKLAQIGNRSETTTGTVNPPVYFSTAYRHEGIGKSTGFDYSRTGNPTRGLLEQAI
CCHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ADLEYGEQGYACSSGMAAVLLVLSLFRSGDELIVSEDLYGGTYRLFSEHEKKWNVRCRYV
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEE
NTQSIKQIEQAITTETKAIFIETPTNPLMQVTDIAAVATVAKRHGLLLIVDNTFYTPYIQ
CHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
QPLTEGADIVLHSATKYLGGHNDVLSGLVVAKGKELCEEIAHYHNASGAVLSPFDSWLLI
HHHCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEECCHHHHHHH
RGMKTLALRMRQHEKNAKAVVAYLNDEDGVTDVFYPGRGGMISFRLKDETWINPFLQSLS
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHH
LITFAESLGGVESLMTYPATQTHADIPEEIRTANGVCNRLLRFSVGIENSNDLIQDLQQA
HHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKLVKEGVRI
HHHHHHCCCC
>Mature Secondary Structure 
STIETKLAQIGNRSETTTGTVNPPVYFSTAYRHEGIGKSTGFDYSRTGNPTRGLLEQAI
CHHHHHHHHCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCCCCCCCCCCCCHHHHHHHHH
ADLEYGEQGYACSSGMAAVLLVLSLFRSGDELIVSEDLYGGTYRLFSEHEKKWNVRCRYV
HHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEE
NTQSIKQIEQAITTETKAIFIETPTNPLMQVTDIAAVATVAKRHGLLLIVDNTFYTPYIQ
CHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
QPLTEGADIVLHSATKYLGGHNDVLSGLVVAKGKELCEEIAHYHNASGAVLSPFDSWLLI
HHHCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCEECCHHHHHHH
RGMKTLALRMRQHEKNAKAVVAYLNDEDGVTDVFYPGRGGMISFRLKDETWINPFLQSLS
HHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHH
LITFAESLGGVESLMTYPATQTHADIPEEIRTANGVCNRLLRFSVGIENSNDLIQDLQQA
HHHHHHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
IKLVKEGVRI
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]