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The map label for this gene is bioD

Identifier: 49187025

GI number: 49187025

Start: 3962459

End: 3963187

Strand: Reverse

Name: bioD

Synonym: BAS4027

Alternate gene names: 49187025

Gene position: 3963187-3962459 (Counterclockwise)

Preceding gene: 49187026

Following gene: 49187024

Centisome position: 75.8

GC content: 38.27

Gene sequence:

>729_bases
ATGAGCGGTTTTTTTATAACAGCAACGGATACTGAAGTGGGAAAAACTGTAGTTGCAGGTGCATTAGCAGGTGTATTTAG
AGAATTGGGATATAACATCGGGGTCTATAAAGCGTTGCAAAGTGGACATGTTGCATCAAATCCAGAGGGAGATGCAGCAA
GGCTAAAAGTATTATCAGGTGTACCGATAAAAGAAGATGAAATTTGTCCTTATTCTATTGAAGAACCGCTTGCTCCGAGA
CTTGCCATGAAAAGAGCTGGAAGGGCAGTAACGTTAAAAGACATTATTCACCACTATAATGAGCGATTGAAAGAGTTTAA
TAGCCTATTTGTAGAAGGTGCAGGTGGACTAGCTGTTCCATATACAGAAGACGCTTTAGTAATTGATTTCGCAAAAGAAT
TACAGCTTCCACTTATTGTAGTAGCACGTCCTACATTAGGAACAGTAAATCATACAGTTTTAACGATTGCTTATGCAAAG
GCACATGGCTTAACAGTAGCAGGTGTCATTTTATCTGGCTGTAAAGAATGCGAAATGGAAAGGGTACAAGAAAATAAAGT
AATGATTGAAGAGCTAAGCGGGGTACCGGTTTTAGGCTTATTACCATTCTTTGAAGGTGAGTTTACAAAAGAAGAAGTAT
TGGAATCTGCTAAAGAATATATAATGATTTCAAAATTAGAGGAGTTCATCCGAAATGAATCAACCGTGGCGCACACATCT
TCAAACTAA

Upstream 100 bases:

>100_bases
CGATTGTATTTATGCCTCCCCTTGCTTCTACAATTGATGAGATTGATGAGATGTTACGTATTTTATATAAAGCAATCTCG
GATATTACGGAGGGAGAATA

Downstream 100 bases:

>100_bases
GTTAAAACAATTACATGAGCAAGGGCAGTATCGCAATTTGCACGTAACAGAACAAGCAGAAGAGACATGGCTTATTCGAG
ATGAGAAAAGGATGCTAAAT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSGFFITATDTEVGKTVVAGALAGVFRELGYNIGVYKALQSGHVASNPEGDAARLKVLSGVPIKEDEICPYSIEEPLAPR
LAMKRAGRAVTLKDIIHHYNERLKEFNSLFVEGAGGLAVPYTEDALVIDFAKELQLPLIVVARPTLGTVNHTVLTIAYAK
AHGLTVAGVILSGCKECEMERVQENKVMIEELSGVPVLGLLPFFEGEFTKEEVLESAKEYIMISKLEEFIRNESTVAHTS
SN

Sequences:

>Translated_242_residues
MSGFFITATDTEVGKTVVAGALAGVFRELGYNIGVYKALQSGHVASNPEGDAARLKVLSGVPIKEDEICPYSIEEPLAPR
LAMKRAGRAVTLKDIIHHYNERLKEFNSLFVEGAGGLAVPYTEDALVIDFAKELQLPLIVVARPTLGTVNHTVLTIAYAK
AHGLTVAGVILSGCKECEMERVQENKVMIEELSGVPVLGLLPFFEGEFTKEEVLESAKEYIMISKLEEFIRNESTVAHTS
SN
>Mature_241_residues
SGFFITATDTEVGKTVVAGALAGVFRELGYNIGVYKALQSGHVASNPEGDAARLKVLSGVPIKEDEICPYSIEEPLAPRL
AMKRAGRAVTLKDIIHHYNERLKEFNSLFVEGAGGLAVPYTEDALVIDFAKELQLPLIVVARPTLGTVNHTVLTIAYAKA
HGLTVAGVILSGCKECEMERVQENKVMIEELSGVPVLGLLPFFEGEFTKEEVLESAKEYIMISKLEEFIRNESTVAHTSS
N

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=205, Percent_Identity=30.7317073170732, Blast_Score=95, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324385, Length=182, Percent_Identity=34.0659340659341, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_BACAA (C3P7Q4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868419.1
- ProteinModelPortal:   C3P7Q4
- SMR:   C3P7Q4
- EnsemblBacteria:   EBBACT00000127485
- GeneID:   7851468
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4362
- GeneTree:   EBGT00050000001962
- ProtClustDB:   PRK00090
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 26295; Mature: 26163

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFFITATDTEVGKTVVAGALAGVFRELGYNIGVYKALQSGHVASNPEGDAARLKVLSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHEEEECC
VPIKEDEICPYSIEEPLAPRLAMKRAGRAVTLKDIIHHYNERLKEFNSLFVEGAGGLAVP
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHEEECCCCEECC
YTEDALVIDFAKELQLPLIVVARPTLGTVNHTVLTIAYAKAHGLTVAGVILSGCKECEME
CCCCEEEEEEHHHCCCCEEEEECCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHH
RVQENKVMIEELSGVPVLGLLPFFEGEFTKEEVLESAKEYIMISKLEEFIRNESTVAHTS
HHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
SN
CC
>Mature Secondary Structure 
SGFFITATDTEVGKTVVAGALAGVFRELGYNIGVYKALQSGHVASNPEGDAARLKVLSG
CCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCHHHEEEECC
VPIKEDEICPYSIEEPLAPRLAMKRAGRAVTLKDIIHHYNERLKEFNSLFVEGAGGLAVP
CCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHEEECCCCEECC
YTEDALVIDFAKELQLPLIVVARPTLGTVNHTVLTIAYAKAHGLTVAGVILSGCKECEME
CCCCEEEEEEHHHCCCCEEEEECCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHHHHHHHH
RVQENKVMIEELSGVPVLGLLPFFEGEFTKEEVLESAKEYIMISKLEEFIRNESTVAHTS
HHHHHHHHHHHHCCCCEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
SN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA