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The map label for this gene is bioH [H]

Identifier: 49187023

GI number: 49187023

Start: 3960569

End: 3961309

Strand: Reverse

Name: bioH [H]

Synonym: BAS4025

Alternate gene names: 49187023

Gene position: 3961309-3960569 (Counterclockwise)

Preceding gene: 49187024

Following gene: 49187022

Centisome position: 75.76

GC content: 35.63

Gene sequence:

>741_bases
ATGAAAGAGCTGAAAATCATTTTTATCCCTGGATGGGGAATGGAAGAAAATATTTGGGATTTAGTACTTCCACATTTTAA
AGGATATTCTGTTCAATGCATTGATTGGCGTAACGTGAAAGAACAAAGTGAATTTGCTGGGCGAATCATAGATGTAGCAA
AGGATGAAAATGTAATTTTAGTTGGATGGTCACTAGGAGCGTTAGCGGCAATTCAAGCTTATAAAAAAATTAAGGCAAAG
GGTATCGTACTTATTGGTGGTACTGCCAAATTTACAAATGCAAGTGACTATAGTAATGGTTGGAATACTTTGCATGTAGA
ACGGCTGAAAAGAAATTTGGCGAGAAGGAAAGAAGATACGCTCAAGCGGTTCTATGAAAATATGTTTACGAAAGATGAGT
TGAAAGAAAATAAAAGTTTTGAAGACATCGTAAAACATTTTAAAGGTGATTCTATCCAGTCTTTACAATTCGGTTTGGAT
TATTTAATAGAAACAGATATGAGAGAAGCATTAAAAGAAATTAACGTCCCTATACTACTTATTCACGGAGAGCGGGATGT
AATATGCCCATTGTCTGCAGCATGTAGTATGGCTGAGAATGAGACGGCCAAGCTTAAGGTAGTAAGTGAGGCTGGACACG
CATTATGTGTAACGAATTTTGAATATTGTGTAAATGAGATAATTCAATTTGTAGAGGGGATACGACATGATCAACAAAAC
GTTACTACAAAAACGGTTTAA

Upstream 100 bases:

>100_bases
CTCGAATTCGCTTTGCAGTTACATCTCAACATACAATAGCTGATTTAAAATGGGCTATTGACCGAATTACCCACATTGCG
AAAGAAGAGGAGCTTTTCGT

Downstream 100 bases:

>100_bases
CGTGGCAGCTGTATCCTACGATCAATATGCAAATGTACAAAAAAAGATGGCACATTCATTACTTTCTACATTAGATCGGC
GATATAGTGCAAATTCATCG

Product: bioH protein

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKELKIIFIPGWGMEENIWDLVLPHFKGYSVQCIDWRNVKEQSEFAGRIIDVAKDENVILVGWSLGALAAIQAYKKIKAK
GIVLIGGTAKFTNASDYSNGWNTLHVERLKRNLARRKEDTLKRFYENMFTKDELKENKSFEDIVKHFKGDSIQSLQFGLD
YLIETDMREALKEINVPILLIHGERDVICPLSAACSMAENETAKLKVVSEAGHALCVTNFEYCVNEIIQFVEGIRHDQQN
VTTKTV

Sequences:

>Translated_246_residues
MKELKIIFIPGWGMEENIWDLVLPHFKGYSVQCIDWRNVKEQSEFAGRIIDVAKDENVILVGWSLGALAAIQAYKKIKAK
GIVLIGGTAKFTNASDYSNGWNTLHVERLKRNLARRKEDTLKRFYENMFTKDELKENKSFEDIVKHFKGDSIQSLQFGLD
YLIETDMREALKEINVPILLIHGERDVICPLSAACSMAENETAKLKVVSEAGHALCVTNFEYCVNEIIQFVEGIRHDQQN
VTTKTV
>Mature_246_residues
MKELKIIFIPGWGMEENIWDLVLPHFKGYSVQCIDWRNVKEQSEFAGRIIDVAKDENVILVGWSLGALAAIQAYKKIKAK
GIVLIGGTAKFTNASDYSNGWNTLHVERLKRNLARRKEDTLKRFYENMFTKDELKENKSFEDIVKHFKGDSIQSLQFGLD
YLIETDMREALKEINVPILLIHGERDVICPLSAACSMAENETAKLKVVSEAGHALCVTNFEYCVNEIIQFVEGIRHDQQN
VTTKTV

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28056; Mature: 28056

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00120 LIPASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELKIIFIPGWGMEENIWDLVLPHFKGYSVQCIDWRNVKEQSEFAGRIIDVAKDENVIL
CCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHEEEECCCCEEE
VGWSLGALAAIQAYKKIKAKGIVLIGGTAKFTNASDYSNGWNTLHVERLKRNLARRKEDT
EECCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
LKRFYENMFTKDELKENKSFEDIVKHFKGDSIQSLQFGLDYLIETDMREALKEINVPILL
HHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHGERDVICPLSAACSMAENETAKLKVVSEAGHALCVTNFEYCVNEIIQFVEGIRHDQQN
EECCCCEECCHHHHHHHCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCC
VTTKTV
CCCCCC
>Mature Secondary Structure
MKELKIIFIPGWGMEENIWDLVLPHFKGYSVQCIDWRNVKEQSEFAGRIIDVAKDENVIL
CCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHEEEECCCCEEE
VGWSLGALAAIQAYKKIKAKGIVLIGGTAKFTNASDYSNGWNTLHVERLKRNLARRKEDT
EECCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHH
LKRFYENMFTKDELKENKSFEDIVKHFKGDSIQSLQFGLDYLIETDMREALKEINVPILL
HHHHHHHHCCHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IHGERDVICPLSAACSMAENETAKLKVVSEAGHALCVTNFEYCVNEIIQFVEGIRHDQQN
EECCCCEECCHHHHHHHCCCCCEEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCC
VTTKTV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA