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The map label for this gene is nudF [H]

Identifier: 49187002

GI number: 49187002

Start: 3942604

End: 3943143

Strand: Reverse

Name: nudF [H]

Synonym: BAS4004

Alternate gene names: 49187002

Gene position: 3943143-3942604 (Counterclockwise)

Preceding gene: 49187004

Following gene: 49187000

Centisome position: 75.41

GC content: 36.11

Gene sequence:

>540_bases
ATGAGTAATCTTGCAGAGAGAACAGTAAAAACTGAGCCGATTTTTGATGGTAGAGTTATAAAAGTTCGTGTTGATGATGT
AGTATTACCAAATGGAGCAATGAGTAAACGTGAAATTGTAAATCACCCTGGTGCAGTTGCTATTATTGCTATTACGGATG
AGGGGAAAATTGTGCTTGTTGAGCAGTATCGTAAAGCGCTTGAAAAGGCGATTATAGAAATTCCGGCTGGCAAGTTAGAA
CCCGGTGAAAAACCTGAGGTGACAGCAGTTCGTGAATTAGAAGAGGAAACAGGTTATGTATGTGAAAATATGGAGCTTAT
TACTTCTTTCTATACATCTCCAGGATTTGCAGATGAAATTTTATATGTATATAAAGCGACAGGTTTGACGAAAAAAGAAA
ATAAAGCTGCGTTAGATGAAGATGAGTTTGTGGAATTGATGGAAGTATCATTAGAAGAAGCAACTACTCTTATGAAAGAT
CTTCGTATTCATGATGCGAAGACGATGTTTGCAGTACAATATTTACAACTACAAAAATAA

Upstream 100 bases:

>100_bases
CGATTTGGTAAAATATAAACTTTCATTTCTATCACGCTCCTTTCAATGTGGTAAAATGTATGAGGAAAGAATAGGAAATA
GAACAGGGAGATGAAGTAGT

Downstream 100 bases:

>100_bases
ACACTCGCCAAAGGCGAGTGTTTTTTAATTGATTTTTGCATATACGCGTGCATCACGTAAGCTACCATCTGGTGCTATGA
AATCATTTTCCATTGTACCT

Product: mutT/nudix family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSNLAERTVKTEPIFDGRVIKVRVDDVVLPNGAMSKREIVNHPGAVAIIAITDEGKIVLVEQYRKALEKAIIEIPAGKLE
PGEKPEVTAVRELEEETGYVCENMELITSFYTSPGFADEILYVYKATGLTKKENKAALDEDEFVELMEVSLEEATTLMKD
LRIHDAKTMFAVQYLQLQK

Sequences:

>Translated_179_residues
MSNLAERTVKTEPIFDGRVIKVRVDDVVLPNGAMSKREIVNHPGAVAIIAITDEGKIVLVEQYRKALEKAIIEIPAGKLE
PGEKPEVTAVRELEEETGYVCENMELITSFYTSPGFADEILYVYKATGLTKKENKAALDEDEFVELMEVSLEEATTLMKD
LRIHDAKTMFAVQYLQLQK
>Mature_178_residues
SNLAERTVKTEPIFDGRVIKVRVDDVVLPNGAMSKREIVNHPGAVAIIAITDEGKIVLVEQYRKALEKAIIEIPAGKLEP
GEKPEVTAVRELEEETGYVCENMELITSFYTSPGFADEILYVYKATGLTKKENKAALDEDEFVELMEVSLEEATTLMKDL
RIHDAKTMFAVQYLQLQK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788810, Length=149, Percent_Identity=31.5436241610738, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20077; Mature: 19946

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLAERTVKTEPIFDGRVIKVRVDDVVLPNGAMSKREIVNHPGAVAIIAITDEGKIVLV
CCCHHHHHCCCCCCCCCEEEEEEECCEECCCCCCCHHHHHCCCCCEEEEEEECCCCEEEH
EQYRKALEKAIIEIPAGKLEPGEKPEVTAVRELEEETGYVCENMELITSFYTSPGFADEI
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHH
LYVYKATGLTKKENKAALDEDEFVELMEVSLEEATTLMKDLRIHDAKTMFAVQYLQLQK
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SNLAERTVKTEPIFDGRVIKVRVDDVVLPNGAMSKREIVNHPGAVAIIAITDEGKIVLV
CCHHHHHCCCCCCCCCEEEEEEECCEECCCCCCCHHHHHCCCCCEEEEEEECCCCEEEH
EQYRKALEKAIIEIPAGKLEPGEKPEVTAVRELEEETGYVCENMELITSFYTSPGFADEI
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHH
LYVYKATGLTKKENKAALDEDEFVELMEVSLEEATTLMKDLRIHDAKTMFAVQYLQLQK
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]