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The map label for this gene is comEC [H]

Identifier: 49186964

GI number: 49186964

Start: 3911511

End: 3912413

Strand: Reverse

Name: comEC [H]

Synonym: BAS3966

Alternate gene names: 49186964

Gene position: 3912413-3911511 (Counterclockwise)

Preceding gene: 49186965

Following gene: 49186963

Centisome position: 74.83

GC content: 36.77

Gene sequence:

>903_bases
ATGAGAGGTATACGAAGTATTTTATTATTAGTTTCTGTTTTGTTATGCTTTTCTTTAAATAGTGCATCGGCAAAAAGGTA
TATGATGTCTATTCATACTGCGTCTCCTTTACATATGCAGCGGCAACATTTTGGTAAAATGAAAGTGAGTTTCTTAAAAG
TTGGACAAGGTGATGCAACACTTATTACATTGCCAAATGGACAAACGATGTTAATTGATGGAGGGCCTTATGAAGCTGGT
GAGGTTATTATCCAAAAGCTAATTGAAAAAGGAATTAACCATTTAGATGCTATCGTCAGTACTCATCCTGATATGGATCA
CATTGGGGGACTCATTCCAATTGTAGAGCAAATGCCAGTATCACTTATATTAGATAGTGGAAAAACATATAGTTCTCTTA
CTTATCATACGTATCGAAATAATATAAAGAAAAGGGGAATCCCTTTCATTTCAGTAAAAGAGGGCCAATACATACCGCTA
GATCCGCATGTTTCCATACAAGTATTAAACAATGGGAAGTCTAAAGATGAAAATAATGAATCTTCAATTGTATTAAAGGT
TCGGTATGGTAAGGCAGATTTTTTACTAATGGGTGATGCTGATGTGCGGACGGAAACGGAAATATTAAAGCAATTTGATG
TACATGCAGATGTGTTAAAGGTGGGGCACCATGGCTCGTATACTTCAACAAGTGAAAGGTTTATAAAGAAGGTAGAACCA
CAGTTCGCGATTCTTTCTTATGGTAGCAGAAATCCGTATGGGCATCCGCATCAAAGTGTAGTGAGACGATTAAAACGGCG
TGGTATAATGATGTATGGAACAAATAGACGTACGGTAGAAATGGAAACAGACGGCGAACATATTATAATAGGGTCTAGCG
GGTTAATGCCATTATTGAAGTGA

Upstream 100 bases:

>100_bases
TTTACAAGAGATATCGAAATAAAAGCTTAGCATTTTGCTAAGCTTTTTTTGTATATCAATTATATACCGTGCCATACTAA
TTGAAAAAAGGAGACGAGTT

Downstream 100 bases:

>100_bases
GTAATCTGATAAGGGGATATTCTCCCTTTTTGGAAAAGTGAATAACATATATTATAATTACAACAGACGTACTAAGTTTC
CTTTTTGTTAAACAAAGAGG

Product: metallo-beta-lactamase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MRGIRSILLLVSVLLCFSLNSASAKRYMMSIHTASPLHMQRQHFGKMKVSFLKVGQGDATLITLPNGQTMLIDGGPYEAG
EVIIQKLIEKGINHLDAIVSTHPDMDHIGGLIPIVEQMPVSLILDSGKTYSSLTYHTYRNNIKKRGIPFISVKEGQYIPL
DPHVSIQVLNNGKSKDENNESSIVLKVRYGKADFLLMGDADVRTETEILKQFDVHADVLKVGHHGSYTSTSERFIKKVEP
QFAILSYGSRNPYGHPHQSVVRRLKRRGIMMYGTNRRTVEMETDGEHIIIGSSGLMPLLK

Sequences:

>Translated_300_residues
MRGIRSILLLVSVLLCFSLNSASAKRYMMSIHTASPLHMQRQHFGKMKVSFLKVGQGDATLITLPNGQTMLIDGGPYEAG
EVIIQKLIEKGINHLDAIVSTHPDMDHIGGLIPIVEQMPVSLILDSGKTYSSLTYHTYRNNIKKRGIPFISVKEGQYIPL
DPHVSIQVLNNGKSKDENNESSIVLKVRYGKADFLLMGDADVRTETEILKQFDVHADVLKVGHHGSYTSTSERFIKKVEP
QFAILSYGSRNPYGHPHQSVVRRLKRRGIMMYGTNRRTVEMETDGEHIIIGSSGLMPLLK
>Mature_300_residues
MRGIRSILLLVSVLLCFSLNSASAKRYMMSIHTASPLHMQRQHFGKMKVSFLKVGQGDATLITLPNGQTMLIDGGPYEAG
EVIIQKLIEKGINHLDAIVSTHPDMDHIGGLIPIVEQMPVSLILDSGKTYSSLTYHTYRNNIKKRGIPFISVKEGQYIPL
DPHVSIQVLNNGKSKDENNESSIVLKVRYGKADFLLMGDADVRTETEILKQFDVHADVLKVGHHGSYTSTSERFIKKVEP
QFAILSYGSRNPYGHPHQSVVRRLKRRGIMMYGTNRRTVEMETDGEHIIIGSSGLMPLLK

Specific function: The comE operon is required for the binding and uptake of transforming DNA. ComEC is required for internalization but is dispensable for DNA binding [H]

COG id: COG2333

COG function: function code R; Predicted hydrolase (metallo-beta-lactamase superfamily)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To H.influenzae REC2, N.gonorrhoeae ComA and E.coli YcaI [H]

Homologues:

Organism=Escherichia coli, GI87081801, Length=290, Percent_Identity=25.8620689655172, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004477
- InterPro:   IPR004797 [H]

Pfam domain/function: PF03772 Competence; PF00753 Lactamase_B [H]

EC number: NA

Molecular weight: Translated: 33522; Mature: 33522

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGIRSILLLVSVLLCFSLNSASAKRYMMSIHTASPLHMQRQHFGKMKVSFLKVGQGDAT
CCHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCEE
LITLPNGQTMLIDGGPYEAGEVIIQKLIEKGINHLDAIVSTHPDMDHIGGLIPIVEQMPV
EEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCCE
SLILDSGKTYSSLTYHTYRNNIKKRGIPFISVKEGQYIPLDPHVSIQVLNNGKSKDENNE
EEEEECCCCCHHEEEHHHHHCHHHCCCCEEEECCCCEECCCCCEEEEEEECCCCCCCCCC
SSIVLKVRYGKADFLLMGDADVRTETEILKQFDVHADVLKVGHHGSYTSTSERFIKKVEP
CEEEEEEEECCEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCC
QFAILSYGSRNPYGHPHQSVVRRLKRRGIMMYGTNRRTVEMETDGEHIIIGSSGLMPLLK
CEEEEECCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MRGIRSILLLVSVLLCFSLNSASAKRYMMSIHTASPLHMQRQHFGKMKVSFLKVGQGDAT
CCHHHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCHHHHHHHHCCEEEEEEEECCCCEE
LITLPNGQTMLIDGGPYEAGEVIIQKLIEKGINHLDAIVSTHPDMDHIGGLIPIVEQMPV
EEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHCCE
SLILDSGKTYSSLTYHTYRNNIKKRGIPFISVKEGQYIPLDPHVSIQVLNNGKSKDENNE
EEEEECCCCCHHEEEHHHHHCHHHCCCCEEEECCCCEECCCCCEEEEEEECCCCCCCCCC
SSIVLKVRYGKADFLLMGDADVRTETEILKQFDVHADVLKVGHHGSYTSTSERFIKKVEP
CEEEEEEEECCEEEEEECCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCHHHHHHHHCCC
QFAILSYGSRNPYGHPHQSVVRRLKRRGIMMYGTNRRTVEMETDGEHIIIGSSGLMPLLK
CEEEEECCCCCCCCCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968523; 8969508; 9384377 [H]