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The map label for this gene is ykwC [H]

Identifier: 49186951

GI number: 49186951

Start: 3898113

End: 3898418

Strand: Reverse

Name: ykwC [H]

Synonym: BAS3953

Alternate gene names: 49186951

Gene position: 3898418-3898113 (Counterclockwise)

Preceding gene: 49186952

Following gene: 49186950

Centisome position: 74.56

GC content: 34.64

Gene sequence:

>306_bases
ATGGTTCATCATTTAATGCAAGAAGGTCATAAGGTATATGTATATAATAGAACGAAAGCGAAAACAGATTCTTTAGTGCA
AGATGGTGCAAATTGGTGTGATACACCGAAAGACTTAGTAAAGCAAGTGGATGTTGTAATGACTATGGTAGGATATCCAC
ATGATGTAGAAGAAGTGTATTTTGGGATAGATGGGATTTTGGAGAATGCAAATGAAGGTACAATTACAATTGATTTCACG
ACATCTACACCAACATTGGCAAAACGTATTAATGAAGCGGGTAAAAGTAAGAATATATATCGTTAG

Upstream 100 bases:

>100_bases
CTGAAATATAGTAAGAGAGTTATTAGAACAAAGGGGATGAGAAAATGGAACATAAAACTTTATCAATAGGTTTCATCGGT
ATTGGTGTAATGGGAAAAGT

Downstream 100 bases:

>100_bases
ATGCGCCTGTATCTGGAGGAGACGTCGGTGCGAAAGAGGCAAGACTCGCAATTATGGTCGGTGGCGAGAAAGAAATATAT
GAAAAATGTTTACCGTTATT

Product: 3-hydroxyisobutyrate dehydrogenase, N-terminus

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 101; Mature: 101

Protein sequence:

>101_residues
MVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFT
TSTPTLAKRINEAGKSKNIYR

Sequences:

>Translated_101_residues
MVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFT
TSTPTLAKRINEAGKSKNIYR
>Mature_101_residues
MVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVYFGIDGILENANEGTITIDFT
TSTPTLAKRINEAGKSKNIYR

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI145693186, Length=99, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 11403; Mature: 11403

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVY
CCCCHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
FGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR
HHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVHHLMQEGHKVYVYNRTKAKTDSLVQDGANWCDTPKDLVKQVDVVMTMVGYPHDVEEVY
CCCCHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
FGIDGILENANEGTITIDFTTSTPTLAKRINEAGKSKNIYR
HHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]