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The map label for this gene is ykwC [H]

Identifier: 49186950

GI number: 49186950

Start: 3897595

End: 3898059

Strand: Reverse

Name: ykwC [H]

Synonym: BAS3952

Alternate gene names: 49186950

Gene position: 3898059-3897595 (Counterclockwise)

Preceding gene: 49186951

Following gene: 49186949

Centisome position: 74.55

GC content: 36.99

Gene sequence:

>465_bases
ATGGTCGGTGGCGAGAAAGAAATATATGAAAAATGTTTACCGTTATTTGAAAAACTAGGAACAAATATTCAGTTGCAAGG
ACCAGCTGGAAGTGGACAACATACAAAAATGTGCAATCAAATTGCAATTGCTTCTAATATGATTGGAGTATGTGAAGCGG
TTGCTTATGCGAAAAAAGCTGGACTAGATCCAGATAAAGTATTAGAGAGTATTTCAACGGGGGCAGCAGGTAGCTGGTCA
TTGAGTAATTTAGCTCCTCGCATGTTAAAGGGAGATTTTGAACCGGGGTTTTATGTAAAGCATTTTATGAAAGATATGAA
GATTGCTTTAGATGAGGCTGAAAAATTACAATTACCAGTACCAGGTTTAAGTTTGGCGAAGGAATTGTATGAAGAGCTAA
TAAAAGATGGAGAAGAGAATAGCGGAACACAAGTGTTATACAAAAAATACATAGGGGGTAAGTGA

Upstream 100 bases:

>100_bases
CAAAACGTATTAATGAAGCGGGTAAAAGTAAGAATATATATCGTTAGATGCGCCTGTATCTGGAGGAGACGTCGGTGCGA
AAGAGGCAAGACTCGCAATT

Downstream 100 bases:

>100_bases
GATGGATCTTCAAAAATTTGATGAGATGATTGATGCTGTACAACGAGCAACTTGTGTACAAATTAATGAAAAACAAATAG
AAGCTTTTAAACAAAAATAT

Product: 3-hydroxyisobutyrate dehydrogenase, C-terminus

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWS
LSNLAPRMLKGDFEPGFYVKHFMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK

Sequences:

>Translated_154_residues
MVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWS
LSNLAPRMLKGDFEPGFYVKHFMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
>Mature_154_residues
MVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKAGLDPDKVLESISTGAAGSWS
LSNLAPRMLKGDFEPGFYVKHFMKDMKIALDEAEKLQLPVPGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=144, Percent_Identity=29.1666666666667, Blast_Score=68, Evalue=3e-12,
Organism=Homo sapiens, GI40556376, Length=151, Percent_Identity=23.1788079470199, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1786719, Length=137, Percent_Identity=40.8759124087591, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI145693186, Length=152, Percent_Identity=33.5526315789474, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1790315, Length=134, Percent_Identity=29.8507462686567, Blast_Score=61, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24655230, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922568, Length=154, Percent_Identity=31.8181818181818, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 16714; Mature: 16714

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKA
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFMKDMKIALDEAEKLQLPV
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MVGGEKEIYEKCLPLFEKLGTNIQLQGPAGSGQHTKMCNQIAIASNMIGVCEAVAYAKKA
CCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLDPDKVLESISTGAAGSWSLSNLAPRMLKGDFEPGFYVKHFMKDMKIALDEAEKLQLPV
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
PGLSLAKELYEELIKDGEENSGTQVLYKKYIGGK
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]