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The map label for this gene is dapL

Identifier: 49186888

GI number: 49186888

Start: 3839301

End: 3840431

Strand: Reverse

Name: dapL

Synonym: BAS3890

Alternate gene names: 49186888

Gene position: 3840431-3839301 (Counterclockwise)

Preceding gene: 49186889

Following gene: 49186887

Centisome position: 73.45

GC content: 38.73

Gene sequence:

>1131_bases
ATGGCAGTAAGCAAATTCGTTCAAATTCGTAGAGACTTACACAAAATACCGGAAATTGGATTTAAAGAGTGGAAAACACA
ACAGTATATTTTAGATTACATAGGAACGCTTTCGAACGAACATGTGGAAGTGAAGGTATGGAGAACGGGTGTCATTGTTA
AAGTAAAGGGGAAAAATCCAGAAAAAGTTATAGGGTATCGCGCAGATATAGACGGTTTACCAATTACAGAGGAAACTGGA
TATGAGTTCGCTTCTGTTCATGAAGGAATGATGCATGCATGCGGACACGACTTACATACAACAATTGGTTTAGGCCTTCT
AACAGCAGCTGTAACAGAAAGAATAGATGATGACCTTGTATTTCTATTCCAACCAGCTGAAGAAGGGCCTGGAGGGGCTC
TTCCTATGTTAGAGAGCGAAGAGTTAAAAGAATGGAAGCCGAATATAATTCTCGGTCTTCATATTGCACCTGAATACCCT
GTAGGAACAATTGCTACAAAAGAGGGGCTATTATTTGCAAATACATCAGAATTATATGTTGATTTAAAGGGGAAAGGTGG
ACATGCAGCTTACCCTCATACTGCAAATGATATGATTGTCGCAGCTAGTCACCTTGTTACACAGCTTCAGTCCGTTATTA
GTCGTAATGTAAACCCACTTGATAGTGCAGTAATTACGATTGGGAAAATAACAGGCGGTACAGTTCAAAATATCATAGCA
GAAAAGTCTCGATTAGAGGGAACAATCCGAACGTTATCAGTGGAATCAATGAGCCGTGTTAAAAGTAGGATTGAGGCAAT
TGTTGCAGGTATAGAAGCTTCCTTCCAATGTGAGGCCGTTATTGACTACGGAGCGATGTATCATCAAGTATACAACCATG
AAGCATTAACGAGAGAGTTTATGCAATTTGTAAGTGAACAAACTGATATGAAAGTAATTACATGTACTGAGGCAATGACA
GGTGAAGATTTTGGTTATATGCTTCAAGAGATTCCTGGATTTATGTTTTGGCTTGGGGTAAATTCAGAATATGGTTTACA
TCACGCAAAATTAAGACCAGATGAAGAGGCTATTGAGAAAGCTATCGTATTTTTAGATCAATATGTGAAGTGGAAGGGAA
CTAGAAAGTAA

Upstream 100 bases:

>100_bases
ATTAAACAAGAGCTTCGTCAATTAAACCCAGAGAAATAAAAACATAAAAGCGTAAGAGCATAAACAGGCTCTTACGCTTT
TTATAGATAGAGGTGCAAAA

Downstream 100 bases:

>100_bases
ACTTGCTCGCCAACTATGTAACAATTTAAAGTTGGTATTTGTTAGTTTCAAGGTGAAAACATCTTTCATATTTGTTTTGG
ATACACATCGGCATACAAAT

Product: M20/M25/M40 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MAVSKFVQIRRDLHKIPEIGFKEWKTQQYILDYIGTLSNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETG
YEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYP
VGTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMT
GEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLRPDEEAIEKAIVFLDQYVKWKGTRK

Sequences:

>Translated_376_residues
MAVSKFVQIRRDLHKIPEIGFKEWKTQQYILDYIGTLSNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETG
YEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYP
VGTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMT
GEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLRPDEEAIEKAIVFLDQYVKWKGTRK
>Mature_375_residues
AVSKFVQIRRDLHKIPEIGFKEWKTQQYILDYIGTLSNEHVEVKVWRTGVIVKVKGKNPEKVIGYRADIDGLPITEETGY
EFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLVFLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPV
GTIATKEGLLFANTSELYVDLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIAE
KSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREFMQFVSEQTDMKVITCTEAMTG
EDFGYMLQEIPGFMFWLGVNSEYGLHHAKLRPDEEAIEKAIVFLDQYVKWKGTRK

Specific function: Catalyzes the conversion of N-acetyl-diaminopimelate to diaminopimelate and acetate

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapL subfamily

Homologues:

Organism=Escherichia coli, GI87081880, Length=217, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPL_BACAA (C3P6Y7)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868280.1
- ProteinModelPortal:   C3P6Y7
- SMR:   C3P6Y7
- EnsemblBacteria:   EBBACT00000125962
- GeneID:   7851390
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4215
- GeneTree:   EBGT00070000031832
- ProtClustDB:   CLSK873387
- HAMAP:   MF_01692
- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650
- PIRSF:   PIRSF005962
- TIGRFAMs:   TIGR01891

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.47

Molecular weight: Translated: 41897; Mature: 41765

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSKFVQIRRDLHKIPEIGFKEWKTQQYILDYIGTLSNEHVEVKVWRTGVIVKVKGKNP
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCCC
EKVIGYRADIDGLPITEETGYEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLV
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPVGTIATKEGLLFANTSELYV
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREF
HHHHHCHHHEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MQFVSEQTDMKVITCTEAMTGEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLRPDEEAIEK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIVFLDQYVKWKGTRK
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AVSKFVQIRRDLHKIPEIGFKEWKTQQYILDYIGTLSNEHVEVKVWRTGVIVKVKGKNP
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEECEEEEEECCCCC
EKVIGYRADIDGLPITEETGYEFASVHEGMMHACGHDLHTTIGLGLLTAAVTERIDDDLV
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEE
FLFQPAEEGPGGALPMLESEELKEWKPNIILGLHIAPEYPVGTIATKEGLLFANTSELYV
EEEECCCCCCCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCCEEECCCEEEEECCEEEE
DLKGKGGHAAYPHTANDMIVAASHLVTQLQSVISRNVNPLDSAVITIGKITGGTVQNIIA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHEEEEECCCCCHHHHHHH
EKSRLEGTIRTLSVESMSRVKSRIEAIVAGIEASFQCEAVIDYGAMYHQVYNHEALTREF
HHHHHCHHHEEEEHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
MQFVSEQTDMKVITCTEAMTGEDFGYMLQEIPGFMFWLGVNSEYGLHHAKLRPDEEAIEK
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCCEECCCCCCHHHHHH
AIVFLDQYVKWKGTRK
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA