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The map label for this gene is pdhC [H]

Identifier: 49186879

GI number: 49186879

Start: 3829314

End: 3830573

Strand: Reverse

Name: pdhC [H]

Synonym: BAS3881

Alternate gene names: 49186879

Gene position: 3830573-3829314 (Counterclockwise)

Preceding gene: 49186880

Following gene: 49186878

Centisome position: 73.26

GC content: 40.79

Gene sequence:

>1260_bases
GTGGCATTTGAATTTAAACTACCAGATATCGGTGAAGGTATCCACGAAGGTGAAATCGTAAAATGGTTTATTAAACCAGG
CGACGAAGTAAACGAAGACGACGTACTTCTTGAAGTACAAAATGATAAAGCAGTAGTAGAAATTCCTTCTCCTGTTAAAG
GTAAAGTACTTGAAGTACTTGTAGAAGAAGGTACGGTTGCAGTAGTTGGAGATACATTAATTAAATTTGATGCTCCAGGA
TACGAAAACCTTAAATTTAAAGGCGACGATCATGACGAAGCTCCTAAAGCTGAAGCTACTCCAGCAGCAACTGCAGAAGT
AGTAAATGAGCGCGTAATCGCTATGCCATCTGTTCGTAAATATGCTCGTGAAAACGGCGTAGACATTCATAAAGTAGCTG
GTTCTGGTAAGAACGGTCGTATCGTAAAAGCTGACATCGATGCATTTGCAAATGGTGGACAAGCAGTAGCAGCAACTGAG
GCTCCAGCAGCAGTAGAAGCTACTCCAGCAGCAGCGAAAGAAGAAGCACCAAAAGCACAACCAATCCCAGCTGGTGAATA
TCCAGAAACTCGTGAGAAAATGAGTGGTATCCGTAAAGCAATTGCGAAAGCAATGGTTAACTCTAAACATACAGCTCCTC
ACGTAACATTAATGGATGAAGTAGATGTAACTGAACTTGTTGCTCACCGTAAGAAGTTCAAAGCTGTGGCAGCTGACAAA
GGTATTAAATTAACTTACCTTCCATACGTTGTTAAAGCTTTAACATCTGCATTACGTGAATACCCAATGTTAAACACTTC
TTTAGATGATGCTTCTCAAGAAGTAGTTCATAAACATTACTTCAACATCGGTATCGCAGCTGATACAGACAAAGGTCTAT
TAGTACCAGTTGTTAAAGATACAGATCGCAAGTCTATCTTCACAATTTCTAACGAGATCAATGATCTTGCTGGTAAAGCA
CGTGAAGGTCGTTTAGCTCCTGCTGAAATGAAAGGCGCTTCTTGCACAATTACAAACATTGGTTCTGCAGGTGGACAATG
GTTCACTCCAGTTATCAACCACCCAGAAGTAGCAATCCTTGGTATCGGCCGTATCGCTGAGAAACCAGTTGTGAAAAACG
GTGAGATCGTTGCAGCTCCAGTATTAGCATTATCTCTAAGCTTTGACCATCGTTTAATTGACGGCGCAACTGCTCAAAAA
GCATTAAACCAAATTAAACGTCTATTGAATGACCCACAATTATTAGTAATGGAGGCGTAA

Upstream 100 bases:

>100_bases
ACTTCTAATTTTTACTTATTCATGTGCAAAAGAAATCAGCACATGCTGATTTCTTTTGCCATATTCCATAATAAAGAAAT
GAATCAGGGGGCTGAATTCC

Downstream 100 bases:

>100_bases
TAACAATGGTAGTAGGAGATTTCCCAATTGAATTAGATACAGTCGTTGTTGGTGCAGGTCCTGGTGGATACGTTGCGGCA
ATTCGTGCAGCACAATTAGG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPG
YENLKFKGDDHDEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATE
APAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAADK
GIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTISNEINDLAGKA
REGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQK
ALNQIKRLLNDPQLLVMEA

Sequences:

>Translated_419_residues
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPG
YENLKFKGDDHDEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATE
APAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAADK
GIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTISNEINDLAGKA
REGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQK
ALNQIKRLLNDPQLLVMEA
>Mature_418_residues
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVLVEEGTVAVVGDTLIKFDAPGY
ENLKFKGDDHDEAPKAEATPAATAEVVNERVIAMPSVRKYARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATEA
PAAVEATPAAAKEEAPKAQPIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAADKG
IKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKDTDRKSIFTISNEINDLAGKAR
EGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAILGIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKA
LNQIKRLLNDPQLLVMEA

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=421, Percent_Identity=35.6294536817102, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI31711992, Length=440, Percent_Identity=29.0909090909091, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI203098753, Length=452, Percent_Identity=27.8761061946903, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=27.8761061946903, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=34.375, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1786305, Length=433, Percent_Identity=35.3348729792148, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1786946, Length=418, Percent_Identity=30.622009569378, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17537937, Length=422, Percent_Identity=33.175355450237, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI25146366, Length=416, Percent_Identity=32.4519230769231, Blast_Score=190, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=29.1111111111111, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=28.8961038961039, Blast_Score=121, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=428, Percent_Identity=31.5420560747664, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.9058295964126, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=36.6132723112128, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24645909, Length=233, Percent_Identity=33.0472103004292, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44902; Mature: 44771

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
CEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDTLIKFDAPGYENLKFKGDDHDEAPKAEATPAATAEVVNERVIAMPSVRK
HHCCCEEEEECEEEEECCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCEEECCHHHH
YARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATEAPAAVEATPAAAKEEAPKAQ
HHHHCCCEEEEECCCCCCCEEEEEEHHHHCCCCCEEEECCCCCCEECCCCHHHHCCCCCC
PIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAADK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
GIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKD
CCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECC
TDRKSIFTISNEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAIL
CCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCEECCCCCCCCEEEE
GIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLNDPQLLVMEA
ECCHHCCCCCCCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure 
AFEFKLPDIGEGIHEGEIVKWFIKPGDEVNEDDVLLEVQNDKAVVEIPSPVKGKVLEVL
EEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCEEEEECCCCCCHHHHHHH
VEEGTVAVVGDTLIKFDAPGYENLKFKGDDHDEAPKAEATPAATAEVVNERVIAMPSVRK
HHCCCEEEEECEEEEECCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHCCEEECCHHHH
YARENGVDIHKVAGSGKNGRIVKADIDAFANGGQAVAATEAPAAVEATPAAAKEEAPKAQ
HHHHCCCEEEEECCCCCCCEEEEEEHHHHCCCCCEEEECCCCCCEECCCCHHHHCCCCCC
PIPAGEYPETREKMSGIRKAIAKAMVNSKHTAPHVTLMDEVDVTELVAHRKKFKAVAADK
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCC
GIKLTYLPYVVKALTSALREYPMLNTSLDDASQEVVHKHYFNIGIAADTDKGLLVPVVKD
CCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEECCCCCEEEEEECC
TDRKSIFTISNEINDLAGKAREGRLAPAEMKGASCTITNIGSAGGQWFTPVINHPEVAIL
CCCCEEEEEHHHHHHHHCCCCCCCCCHHHCCCCEEEEEECCCCCCCEECCCCCCCCEEEE
GIGRIAEKPVVKNGEIVAAPVLALSLSFDHRLIDGATAQKALNQIKRLLNDPQLLVMEA
ECCHHCCCCCCCCCCEEEEEEEEEEECCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]