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The map label for this gene is pdhD [H]

Identifier: 49186878

GI number: 49186878

Start: 3827896

End: 3829308

Strand: Reverse

Name: pdhD [H]

Synonym: BAS3880

Alternate gene names: 49186878

Gene position: 3829308-3827896 (Counterclockwise)

Preceding gene: 49186879

Following gene: 49186875

Centisome position: 73.24

GC content: 40.76

Gene sequence:

>1413_bases
ATGGTAGTAGGAGATTTCCCAATTGAATTAGATACAGTCGTTGTTGGTGCAGGTCCTGGTGGATACGTTGCGGCAATTCG
TGCAGCACAATTAGGTCAAAAGGTAGCAATTATTGAAAAAGCTAACCTTGGTGGCGTATGCTTAAACGTTGGATGTATTC
CTTCAAAAGCGTTAATCAATGCAGGTCATCGTTATGAGAATGCAATGCATTCTGATGACATGGGTATCACTGCAGAGAAC
GTAAAAGTTGACTTTACAAAAGTTCAAGAATGGAAAAACGGCGTAGTTAAGAAATTAACTGGCGGTGTTGAAGGCCTTCT
TAAAGGTAACAAAGTTGAAATCATTCGCGGTGAAGCTTACTTCGTAGATGCTAATACATTACGCGTTATGACTGAAGAGG
CAGCTCAAACTTATACGTTTAAAAATGCTGTTCTTGCAACTGGTTCTACACCAATCGAAATTCCAGGATTCAAATACTCT
AAACGTGTTATCAACTCTACAGGCGCTTTAAGCTTACCTGAAATTCCTAAAAAACTTGTTGTAATCGGCGGCGGTTACAT
CGGTATGGAATTAGGTACTGCATATGCTAACTTCGGTACAGAAGTTACTGTAGTAGAAGCTGGCGACGAAATCTTAGCTG
GTTTCGAAAAAGCTATGAGCTCTGTTGTTAAACGTGCTCTACAGAAAAAAGGTAACGTAAATATCCATACAAAAGCTATG
GCTAAAGGCGTTGAAGAAACAGAAACTGGCGTAAAAGTTAGCTTTGAAGTTAAAGGTGAAATCCAAACTGTAGAAGCAGA
TTACGTATTAGTAACTGTAGGTCGTCGTCCAAACACTCAAGAAATCGGTCTTGAGCAAGTTGGAGTTAAAATGACTGACC
GCGGCATCATCGAAATCGATGAGCAATGTCGTACAAACGTACCAAACATCTATGCAATCGGTGATATCGTTCCTGGACCA
CCATTAGCTCACAAAGCTTCTTACGAAGGTAAAGTAGCTGTAGAAGCAATTAGTGGCCATGCATCAGCTATCGATTACAT
CGGAATTCCTGCAGTATGCTTCACTGATCCAGAATTAGCATCTGTTGGTTACACTAAGAAACAAGCTGAAGAAGCTGGAA
TGACTGTAACTGTATCTAAGTTCCCATTCGCTGCTAACGGTCGTGCATTATCATTAAACAGCACTGACGGTTTCTTACAA
CTTGTAACACGTAAAGAAGATGGTCTTCTTGTAGGTGCTCAAGTTGCAGGTGCAGGCGCTTCTGATATTATTTCTGAGAT
TGGTTTAGCTATCGAAGCTGGAATGACAGCAGAAGATATCGCTCAAACAATCCACGCTCACCCAACATTAGGTGAAATCA
CAATGGAAGCAGCTGAAGTTGCTCTTGGAATGCCAATTCACATTGTAAAATAA

Upstream 100 bases:

>100_bases
ACCATCGTTTAATTGACGGCGCAACTGCTCAAAAAGCATTAAACCAAATTAAACGTCTATTGAATGACCCACAATTATTA
GTAATGGAGGCGTAATAACA

Downstream 100 bases:

>100_bases
TTTAAGTAATGAAAATAGCCCCTCTCTTTAAAAGAGAGGGGCTATTTTTTATTTTATAATAAAAATGGCTTGCAAGGTCT
TTATTTGTACTTGTCGAATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; S complex, 50 kDa subunit [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALINAGHRYENAMHSDDMGITAEN
VKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM
AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNTQEIGLEQVGVKMTDRGIIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK

Sequences:

>Translated_470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALINAGHRYENAMHSDDMGITAEN
VKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM
AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNTQEIGLEQVGVKMTDRGIIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
>Mature_470_residues
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALINAGHRYENAMHSDDMGITAEN
VKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAYFVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYS
KRVINSTGALSLPEIPKKLVVIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM
AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNTQEIGLEQVGVKMTDRGIIEIDEQCRTNVPNIYAIGDIVPGP
PLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPELASVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQ
LVTRKEDGLLVGAQVAGAGASDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK

Specific function: Catalyzes the oxidation of dihydrolipoamide to lipoamide [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=43.0735930735931, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI50301238, Length=466, Percent_Identity=30.2575107296137, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI291045266, Length=460, Percent_Identity=28.4782608695652, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI33519430, Length=456, Percent_Identity=27.4122807017544, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI33519428, Length=456, Percent_Identity=27.4122807017544, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI33519426, Length=456, Percent_Identity=27.4122807017544, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI148277065, Length=456, Percent_Identity=27.4122807017544, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI148277071, Length=456, Percent_Identity=27.4122807017544, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI22035672, Length=460, Percent_Identity=28.0434782608696, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI291045268, Length=451, Percent_Identity=26.6075388026608, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786307, Length=459, Percent_Identity=44.4444444444444, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=31.3636363636364, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=29.4372294372294, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081717, Length=447, Percent_Identity=28.8590604026846, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789065, Length=195, Percent_Identity=27.1794871794872, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=454, Percent_Identity=42.0704845814978, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI71983429, Length=464, Percent_Identity=30.6034482758621, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983419, Length=464, Percent_Identity=30.6034482758621, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17557007, Length=473, Percent_Identity=28.9640591966173, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71982272, Length=486, Percent_Identity=24.8971193415638, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=202, Percent_Identity=27.7227722772277, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=40.4661016949153, Blast_Score=322, Evalue=8e-89,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=29.3103448275862, Blast_Score=184, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=27.6150627615063, Blast_Score=180, Evalue=5e-46,
Organism=Drosophila melanogaster, GI21358499, Length=469, Percent_Identity=44.136460554371, Blast_Score=354, Evalue=6e-98,
Organism=Drosophila melanogaster, GI24640553, Length=467, Percent_Identity=28.6937901498929, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640549, Length=467, Percent_Identity=28.6937901498929, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640551, Length=467, Percent_Identity=28.6937901498929, Blast_Score=141, Evalue=9e-34,
Organism=Drosophila melanogaster, GI17737741, Length=469, Percent_Identity=26.226012793177, Blast_Score=124, Evalue=1e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49454; Mature: 49454

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALIN
CEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHH
AGHRYENAMHSDDMGITAENVKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY
CCHHHHCCCCCCCCCEEECCEEEEHHHHHHHHCCHHHHHCCCHHHHCCCCEEEEEECCEE
FVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV
EEECCEEEEEEHHHHHHEEECCEEEECCCCCEECCCCCHHHHHHCCCCCCCCCCCCCEEE
VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM
EEECCEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNTQEIGLEQVGVKMTDRGIIEID
HCCCCCCCCCEEEEEEECCEEEEEEEEEEEEEECCCCCCHHCCHHHCCEEEECCCCEEEC
EQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPELA
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEHCCCCCEEEECCCCHH
SVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAGA
HCCCCHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
HHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC
>Mature Secondary Structure
MVVGDFPIELDTVVVGAGPGGYVAAIRAAQLGQKVAIIEKANLGGVCLNVGCIPSKALIN
CEECCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEEEECCCCCHHHHH
AGHRYENAMHSDDMGITAENVKVDFTKVQEWKNGVVKKLTGGVEGLLKGNKVEIIRGEAY
CCHHHHCCCCCCCCCEEECCEEEEHHHHHHHHCCHHHHHCCCHHHHCCCCEEEEEECCEE
FVDANTLRVMTEEAAQTYTFKNAVLATGSTPIEIPGFKYSKRVINSTGALSLPEIPKKLV
EEECCEEEEEEHHHHHHEEECCEEEECCCCCEECCCCCHHHHHHCCCCCCCCCCCCCEEE
VIGGGYIGMELGTAYANFGTEVTVVEAGDEILAGFEKAMSSVVKRALQKKGNVNIHTKAM
EEECCEEEEECCCHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHH
AKGVEETETGVKVSFEVKGEIQTVEADYVLVTVGRRPNTQEIGLEQVGVKMTDRGIIEID
HCCCCCCCCCEEEEEEECCEEEEEEEEEEEEEECCCCCCHHCCHHHCCEEEECCCCEEEC
EQCRTNVPNIYAIGDIVPGPPLAHKASYEGKVAVEAISGHASAIDYIGIPAVCFTDPELA
HHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEHCCCCCEEEECCCCHH
SVGYTKKQAEEAGMTVTVSKFPFAANGRALSLNSTDGFLQLVTRKEDGLLVGAQVAGAGA
HCCCCHHHHHHCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEECCCCEEEEEEECCCCH
SDIISEIGLAIEAGMTAEDIAQTIHAHPTLGEITMEAAEVALGMPIHIVK
HHHHHHHCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377; 1936936 [H]