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The map label for this gene is thrZ [H]

Identifier: 49185231

GI number: 49185231

Start: 2225379

End: 2227298

Strand: Direct

Name: thrZ [H]

Synonym: BAS2222

Alternate gene names: 49185231

Gene position: 2225379-2227298 (Clockwise)

Preceding gene: 49185230

Following gene: 49185232

Centisome position: 42.56

GC content: 34.84

Gene sequence:

>1920_bases
ATGAAAGAACAGATGATTGAAATTAAATTTCCAGATGGTAGCGTGAAAGAATTTGTAAAAGGAATTACTTTAGAAGAAAT
TGCTGGATCCATTAGCAGTAGCTTAAAAAAGAAAGCAGTTGCTGGGAACGTAAATGATGAGTTATATGATTTACGCCGTA
ATATTGAAGAAAATGCGGAAGTAGAAATCATTACTATAGATTCAAATGAAGGAGTAGAAATTGCAAGACACTCCGCGGCA
CATATTTTAGCGCAAGCTGTAAAAAGATTGTATGGTGATATAAATCTTGGAGTAGGGCCTGTTATTGAAAATGGATTTTA
TTATGATATGGATCTTCCGAGTAGTGTAAACGTAGAAGATTTACGAAAAATCGAAAAAGAAATGAAAAAGATTATAAATG
AAAATATAAAGGTAGAACGAGTTGAGGTTTCTCGAGAAGAAGCAGCTAAACTGTTTCAAGAAATGAACGATCGATTGAAA
TTAGAACTTTTAGAAGCAATTCCTAGTGGGGAAAGTGTAACATTATATAAACAAGGTGAATTTGTAGATTTATGTAGAGG
TCCACATTTACCGTCAACAGGTTATTTGAAAGTATTTCAATTAACTCACGTTTCCGGTGCATATTGGCGAGGAGATAGTA
ATAATCAAGTGCTTCAGCGTATATATGGTGTTGCATTCTCTTCTCAGAAAGAATTAGAAGAGTATTTACATTTCGTTGAA
GAAGCAGCCAAAAGAAACCATCGTAAGTTAGGCAATGAACTGGAGCTATTTATGTTTTCTGAAGAGGCTCCGGGAATGCC
GTTTTATTTACCGAAAGGCCAAATTATTCGTAATGAATTAGAAGCTTTTTTAAGAGAAATTCAAAAAGAATATAATTATC
AAGAAGTACGCACTCCATTCATGATGAACCAAGAAGTATGGGAAAGATCAGGGCACTGGGGACATTATAAAGATAATATG
TATTTCTCAGAAGTGGATAATAAAAGTTTTGCATTAAAACCGATGAACTGCCCAGGGCATATGCTAATGTTCAAAAATAA
ATTGCATTCTTATCGCGAATTACCGATTCGTATGTGTGAGTTTGGTCAAGTACATCGCCATGAATTTAGTGGGGCGTTAA
ACGGATTATTAAGAGTACGTACTTTCTGCCAAGATGATGCACATTTATTTGTAACTCCAGAACAAATTGAAGATGAAATC
AAATCTGTAATGGCGCAAATCGATTATGTATATAAAACTTTTGGATTCGAATATGAAGTAGAACTTTCTACTCGTCCAGA
AGATTCAATGGGGGATGATGAGTTATGGGAGCAAGCAGAAGCGTCATTAGAAAATGTATTACAATCGCTAAATTATAAAT
ATAGACTAAATGAAGGTGATGGTGCATTTTACGGACCGAAAATTGACTTCCATATTAAAGATGCTTTAAATAGAAGTCAT
CAGTGTGGGACAATCCAGCTAGATTTCCAAATGCCAGAGAAGTTTGATTTAAATTATATAGATGAGAAGAATGAAAAGAG
AAGACCAGTTGTCATTCATAGGGCAGTATTAGGATCTTTAGATCGTTTCTTAGCAATATTAATTGAGCACTTTGGAGGTG
CATTCCCAGCATGGGTGGCACCAGTTCAAGTGAAAGTCATTCCAGTTTCAAATGCAGTCCATGTACAATACGCAGATGAG
GTTGCAGATAAATTAGCACAAGCTGGTGTTCGTGTCGAACGAGATATGCGAGATGAAAAATTAGGATATAAAATAAGAGA
AGCACAAATGCAAAAAGTTCCTTATGTTCTTGTTATTGGTGATAAGGAAATGGAAAACGGAGCTGTAAATGTGCGTAAAT
ATGGGGAAGAAAAATCAGGTGTAATTGAACTAGATATGTTTGTGGAAAGTATGAAAGAGGAAATAAATAATCGAAAATAG

Upstream 100 bases:

>100_bases
ATGTTTGTGATGGGAATCTGGGTGGAACCACGGGTATAAGCACGCTCGTCCCTATTGTAGGGATGAGTGTGCTTTTTATT
ATGAAATGGAGAGATGAAAA

Downstream 100 bases:

>100_bases
ATTTAAAAAAGAAAACACACCAATTCAAAAATGGTGTGTTTTCTTTTTTACCTTACAAAAATGTAAGATAAATGTAAGCG
AAATCGAAGGAACGAGTTGT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MKEQMIEIKFPDGSVKEFVKGITLEEIAGSISSSLKKKAVAGNVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLPSSVNVEDLRKIEKEMKKIINENIKVERVEVSREEAAKLFQEMNDRLK
LELLEAIPSGESVTLYKQGEFVDLCRGPHLPSTGYLKVFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKELEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDELWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWVAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSGVIELDMFVESMKEEINNRK

Sequences:

>Translated_639_residues
MKEQMIEIKFPDGSVKEFVKGITLEEIAGSISSSLKKKAVAGNVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLPSSVNVEDLRKIEKEMKKIINENIKVERVEVSREEAAKLFQEMNDRLK
LELLEAIPSGESVTLYKQGEFVDLCRGPHLPSTGYLKVFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKELEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDELWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWVAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSGVIELDMFVESMKEEINNRK
>Mature_639_residues
MKEQMIEIKFPDGSVKEFVKGITLEEIAGSISSSLKKKAVAGNVNDELYDLRRNIEENAEVEIITIDSNEGVEIARHSAA
HILAQAVKRLYGDINLGVGPVIENGFYYDMDLPSSVNVEDLRKIEKEMKKIINENIKVERVEVSREEAAKLFQEMNDRLK
LELLEAIPSGESVTLYKQGEFVDLCRGPHLPSTGYLKVFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKELEEYLHFVE
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPFMMNQEVWERSGHWGHYKDNM
YFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCEFGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEI
KSVMAQIDYVYKTFGFEYEVELSTRPEDSMGDDELWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWVAPVQVKVIPVSNAVHVQYADE
VADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIGDKEMENGAVNVRKYGEEKSGVIELDMFVESMKEEINNRK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=632, Percent_Identity=43.6708860759494, Blast_Score=554, Evalue=1e-157,
Organism=Homo sapiens, GI38202255, Length=611, Percent_Identity=45.3355155482815, Blast_Score=552, Evalue=1e-157,
Organism=Homo sapiens, GI20070344, Length=656, Percent_Identity=39.9390243902439, Blast_Score=485, Evalue=1e-137,
Organism=Escherichia coli, GI1788013, Length=636, Percent_Identity=46.8553459119497, Blast_Score=631, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=167, Percent_Identity=24.5508982035928, Blast_Score=63, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=44.3740095087163, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI71984184, Length=631, Percent_Identity=44.3740095087163, Blast_Score=558, Evalue=1e-159,
Organism=Saccharomyces cerevisiae, GI6322112, Length=627, Percent_Identity=43.7001594896332, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6322654, Length=396, Percent_Identity=40.9090909090909, Blast_Score=318, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24583842, Length=635, Percent_Identity=43.4645669291339, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI28574141, Length=635, Percent_Identity=43.4645669291339, Blast_Score=558, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24583839, Length=635, Percent_Identity=43.4645669291339, Blast_Score=558, Evalue=1e-159,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73735; Mature: 73735

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEQMIEIKFPDGSVKEFVKGITLEEIAGSISSSLKKKAVAGNVNDELYDLRRNIEENAE
CCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
VEIITIDSNEGVEIARHSAAHILAQAVKRLYGDINLGVGPVIENGFYYDMDLPSSVNVED
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCHHH
LRKIEKEMKKIINENIKVERVEVSREEAAKLFQEMNDRLKLELLEAIPSGESVTLYKQGE
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
FVDLCRGPHLPSTGYLKVFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKELEEYLHFVE
CHHHHCCCCCCCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPF
HHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCH
MMNQEVWERSGHWGHYKDNMYFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCE
HCCHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
FGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEIKSVMAQIDYVYKTFGFEYEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPEDSMGDDELWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEHHHCCCCC
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWVA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVKVIPVSNAVHVQYADEVADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIG
CEEEEEEECCCEEEEEEHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHCCEEEEEC
DKEMENGAVNVRKYGEEKSGVIELDMFVESMKEEINNRK
CCCCCCCCEEHHHCCCCCCCEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEQMIEIKFPDGSVKEFVKGITLEEIAGSISSSLKKKAVAGNVNDELYDLRRNIEENAE
CCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
VEIITIDSNEGVEIARHSAAHILAQAVKRLYGDINLGVGPVIENGFYYDMDLPSSVNVED
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCHHH
LRKIEKEMKKIINENIKVERVEVSREEAAKLFQEMNDRLKLELLEAIPSGESVTLYKQGE
HHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
FVDLCRGPHLPSTGYLKVFQLTHVSGAYWRGDSNNQVLQRIYGVAFSSQKELEEYLHFVE
CHHHHCCCCCCCCCCEEEEEEEECCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
EAAKRNHRKLGNELELFMFSEEAPGMPFYLPKGQIIRNELEAFLREIQKEYNYQEVRTPF
HHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCHHHHCCCH
MMNQEVWERSGHWGHYKDNMYFSEVDNKSFALKPMNCPGHMLMFKNKLHSYRELPIRMCE
HCCHHHHHCCCCCCCCCCCCEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHHHH
FGQVHRHEFSGALNGLLRVRTFCQDDAHLFVTPEQIEDEIKSVMAQIDYVYKTFGFEYEV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPEDSMGDDELWEQAEASLENVLQSLNYKYRLNEGDGAFYGPKIDFHIKDALNRSH
EECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEEEEEEHHHCCCCC
QCGTIQLDFQMPEKFDLNYIDEKNEKRRPVVIHRAVLGSLDRFLAILIEHFGGAFPAWVA
CCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCHHCC
PVQVKVIPVSNAVHVQYADEVADKLAQAGVRVERDMRDEKLGYKIREAQMQKVPYVLVIG
CEEEEEEECCCEEEEEEHHHHHHHHHHCCCHHHHHCCHHHHCCHHHHHHHHHCCEEEEEC
DKEMENGAVNVRKYGEEKSGVIELDMFVESMKEEINNRK
CCCCCCCCEEHHHCCCCCCCEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 9384377; 9353933; 1379177 [H]