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The map label for this gene is rluF [H]

Identifier: 49185230

GI number: 49185230

Start: 2224011

End: 2224727

Strand: Direct

Name: rluF [H]

Synonym: BAS2221

Alternate gene names: 49185230

Gene position: 2224011-2224727 (Clockwise)

Preceding gene: 49185229

Following gene: 49185231

Centisome position: 42.53

GC content: 34.17

Gene sequence:

>717_bases
GTGCCTAATTTGCGTATCAATAAATTTATTAGTGAAGCTGGAAAAGCGTCTCGACGTGGAGCAGATAAACTAATTAATGA
GAGAAGAGTAATTATTAACGGCAAGGTTGCAAAAATTGGTGACCAAGTGAATCCAGGTGATGATGTACGCGTAAATGGAG
AGCAACTTCGCATTGCTCGAGATCACGTATATATTGCTTTAAATAAACCAGTAGGTATTACATGTACAAGTGAAAAATCG
GTAAAAGGTAATATTATCGATCTAGTGAATCATCCATTACGAATTAGTCACATTGGACGTCTTGATAAAGACTCAGACGG
TTTAATTTTGTTAACGAATGATGGTGATATCGTTAATGAAATTTTACGTGCTGAAAACAAACATGAGAAAGAATATATCG
TTTCAGTAGATAAGCCAATTACACCTGATTTCTTAGAAAAAATGGCAGCTGGTGTGAAAATTTTAGGAACGAAAACACTT
CCATGTGAGATCACACAGTTATCAAAATATGAGTTTAAAATTATTTTAACACAAGGATTGAATCGTCAAATTCGCCGTAT
GTGTGAAGCTTTAGGGTATCAAGTATATACGTTAAAACGTACGAGAATTATGAATATTGAATTGAATAACTTACCAGTTG
GACAGTGGAGAGATTTAACGAAGAAAGAGAAAAGACGTCTATTTTCAGACTTAAATTACGAACCACAAGATTGGTAA

Upstream 100 bases:

>100_bases
TGCCCTTTCATGTTAATATATTTAAGTTATAATAGGAGGCTTACCCTTTTGTATTGTACGGTAGAAGCAGTGTGAGCTTG
AAATTTTTTTAGAAAAGGAT

Downstream 100 bases:

>100_bases
AATTGAGAAGAAGAAACCAAAAATCTATTTTTGGTTTCTTTTTTTATTAAATAAAAAATATTTTTTTAACACTTGCAAAA
ATAAAATGAATAAGATATAG

Product: RNA pseudouridylate synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MPNLRINKFISEAGKASRRGADKLINERRVIINGKVAKIGDQVNPGDDVRVNGEQLRIARDHVYIALNKPVGITCTSEKS
VKGNIIDLVNHPLRISHIGRLDKDSDGLILLTNDGDIVNEILRAENKHEKEYIVSVDKPITPDFLEKMAAGVKILGTKTL
PCEITQLSKYEFKIILTQGLNRQIRRMCEALGYQVYTLKRTRIMNIELNNLPVGQWRDLTKKEKRRLFSDLNYEPQDW

Sequences:

>Translated_238_residues
MPNLRINKFISEAGKASRRGADKLINERRVIINGKVAKIGDQVNPGDDVRVNGEQLRIARDHVYIALNKPVGITCTSEKS
VKGNIIDLVNHPLRISHIGRLDKDSDGLILLTNDGDIVNEILRAENKHEKEYIVSVDKPITPDFLEKMAAGVKILGTKTL
PCEITQLSKYEFKIILTQGLNRQIRRMCEALGYQVYTLKRTRIMNIELNNLPVGQWRDLTKKEKRRLFSDLNYEPQDW
>Mature_237_residues
PNLRINKFISEAGKASRRGADKLINERRVIINGKVAKIGDQVNPGDDVRVNGEQLRIARDHVYIALNKPVGITCTSEKSV
KGNIIDLVNHPLRISHIGRLDKDSDGLILLTNDGDIVNEILRAENKHEKEYIVSVDKPITPDFLEKMAAGVKILGTKTLP
CEITQLSKYEFKIILTQGLNRQIRRMCEALGYQVYTLKRTRIMNIELNNLPVGQWRDLTKKEKRRLFSDLNYEPQDW

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=228, Percent_Identity=58.7719298245614, Blast_Score=256, Evalue=7e-70,
Organism=Escherichia coli, GI1788510, Length=226, Percent_Identity=30.0884955752212, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787524, Length=229, Percent_Identity=31.4410480349345, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI87081838, Length=145, Percent_Identity=31.7241379310345, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27169; Mature: 27038

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLRINKFISEAGKASRRGADKLINERRVIINGKVAKIGDQVNPGDDVRVNGEQLRIAR
CCCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCEEECCCCCCCCCCEEECHHEEEEEE
DHVYIALNKPVGITCTSEKSVKGNIIDLVNHPLRISHIGRLDKDSDGLILLTNDGDIVNE
EEEEEEECCCEEEEECCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHH
ILRAENKHEKEYIVSVDKPITPDFLEKMAAGVKILGTKTLPCEITQLSKYEFKIILTQGL
HHHCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEECCCCCEEEEECCCEEEEEEEECCC
NRQIRRMCEALGYQVYTLKRTRIMNIELNNLPVGQWRDLTKKEKRRLFSDLNYEPQDW
CHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
PNLRINKFISEAGKASRRGADKLINERRVIINGKVAKIGDQVNPGDDVRVNGEQLRIAR
CCCHHHHHHHHHHHHHHCCHHHHHCCCEEEEECCEEECCCCCCCCCCEEECHHEEEEEE
DHVYIALNKPVGITCTSEKSVKGNIIDLVNHPLRISHIGRLDKDSDGLILLTNDGDIVNE
EEEEEEECCCEEEEECCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCEEEEECCCHHHHH
ILRAENKHEKEYIVSVDKPITPDFLEKMAAGVKILGTKTLPCEITQLSKYEFKIILTQGL
HHHCCCCCCCEEEEEECCCCCHHHHHHHHCCEEEEECCCCCEEEEECCCEEEEEEEECCC
NRQIRRMCEALGYQVYTLKRTRIMNIELNNLPVGQWRDLTKKEKRRLFSDLNYEPQDW
CHHHHHHHHHHCCEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]