Click here to switch to the map view.

The map label for this gene is brnQ [H]

Identifier: 49184783

GI number: 49184783

Start: 1796743

End: 1797165

Strand: Direct

Name: brnQ [H]

Synonym: BAS1771

Alternate gene names: 49184783

Gene position: 1796743-1797165 (Clockwise)

Preceding gene: 304570531

Following gene: 49184784

Centisome position: 34.36

GC content: 36.64

Gene sequence:

>423_bases
ATGACGAATAAAGTACCGTTTTCGTTCATAGTTGTTATTGGTTTAATGTTATTTGCACTATTTTTTGGGGCAGGAAATTT
AATTTTTCCGGCAATGCTTGGTCAATCGGCAGGAGAAAATGTATGGATTGCTAACGCTGGATTTTTAGTAACAGGTGTTG
GATTACCATTACTAGGTGTACTAGCATTTGGTTTTTCGGGTAAAGATGATTTACAGTCATTAGCAAGTCGTGCTCACCCA
GTGTTCGGGATTGTGTTTACAACAGTTTTATACTTGGCGATTGGCCCGTTATTTGCAATACCAAGAACAGGAAATGTTTC
TTATGAAATTGGTCTTAAACCGTTTATGCCAGAGGGATTAGGTTCTACACCTTTAATTCTTTTCACAATTGTATTCTTTA
GCATCACTTGTTTTTTTCGCTAA

Upstream 100 bases:

>100_bases
TAATGAGTTCAAGAATAGTTGATACTTAAGTTAAATATTCAGAAAATTCAATTAGTTAAAATTGGAAATGACATAGGTTA
TTTTGAATGGAGGTTATATT

Downstream 100 bases:

>100_bases
ATCCCGCGAAAATTGTCGATATTGTTGGAAAAATCTTAACGCCAATTAAGTTAACTTTCATCGGTATTTTAGTAATCGTT
GCTTTTATTCATCCGATTGG

Product: branched-chain amino acid transporter, N-terminus

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BrnQ [H]

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGFSGKDDLQSLASRAHP
VFGIVFTTVLYLAIGPLFAIPRTGNVSYEIGLKPFMPEGLGSTPLILFTIVFFSITCFFR

Sequences:

>Translated_140_residues
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGFSGKDDLQSLASRAHP
VFGIVFTTVLYLAIGPLFAIPRTGNVSYEIGLKPFMPEGLGSTPLILFTIVFFSITCFFR
>Mature_139_residues
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGVLAFGFSGKDDLQSLASRAHPV
FGIVFTTVLYLAIGPLFAIPRTGNVSYEIGLKPFMPEGLGSTPLILFTIVFFSITCFFR

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=135, Percent_Identity=47.4074074074074, Blast_Score=117, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 14958; Mature: 14827

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGFSGKDDLQSLASRAHPVFGIVFTTVLYLAIGPLFAIPRTGNVSYEIGLKPFMPEGL
HHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GSTPLILFTIVFFSITCFFR
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNKVPFSFIVVIGLMLFALFFGAGNLIFPAMLGQSAGENVWIANAGFLVTGVGLPLLGV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHH
LAFGFSGKDDLQSLASRAHPVFGIVFTTVLYLAIGPLFAIPRTGNVSYEIGLKPFMPEGL
HHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
GSTPLILFTIVFFSITCFFR
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9023181; 9308178; 9384377 [H]