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The map label for this gene is acpD [H]

Identifier: 304570531

GI number: 304570531

Start: 1795811

End: 1796503

Strand: Direct

Name: acpD [H]

Synonym: NA

Alternate gene names: 304570531

Gene position: 1795811-1796503 (Clockwise)

Preceding gene: 49184780

Following gene: 49184783

Centisome position: 34.35

GC content: 34.34

Gene sequence:

>693_bases
ATGGGATTATTTAGCTCGTTATTTGGTAAAAAAGAAGAAAATCAAAAAGTAGAGGAGAATAAAACAATGTCAAAAGTATT
ATTTGTAAAAGCAAACGATCGCCCAGCGGAGCAAGCAGTTAGTTCAAAAATGTATGAAACATTTGTAACTACTTATAAAG
AAGCAAATCCGAATACAGAAATTACAGAGTTAGATTTATTTGCATTAGATCTTCCTTATTACGGAAATATTGCTATTTCA
GGTGGATACAAACGTAGTCAAGGTATGGAATTAACAGCAGAAGAAGAGAAGGCAGTTGCTACAGTAGATCAATATTTAAA
TCAGTTTTTAGAAGCTGATAAAGTTGTATTTGCATTCCCGTTATGGAACTTTACTGTACCAGCACCATTAATCACATATA
TTTCATATTTATCTCAAGCTGGAAAAACGTTTAAATATACAGCTAATGGTCCAGAAGGTTTAGCTGGTGGTAAAAAAGTA
GTTGTGTTAGGTGCTCGTGGTTCAGATTACTCTTCAGAACAAATGGCTCCTATGGAGATGGCAGTTAATTACGTAACAAC
TGTACTTGGATTCTGGGGAATTACAAATCCAGAAACTGTTGTAATTGAAGGACACAACCAATATCCAGATCGCTCACAAC
AAATTGTTGAAGAAGGTTTAGAGAACGTTAAGAAAGTAGCGGCGAAATTTTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: azoreductase

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: Azo-dye reductase 1; FMN-dependent NADH-azo compound oxidoreductase 1 [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:
NA

Sequences:

>Translated_230_residues
MGLFSSLFGKKEENQKVEENKTMSKVLFVKANDRPAEQAVSSKMYETFVTTYKEANPNTEITELDLFALDLPYYGNIAIS
GGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKV
VVLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
>Mature_229_residues
GLFSSLFGKKEENQKVEENKTMSKVLFVKANDRPAEQAVSSKMYETFVTTYKEANPNTEITELDLFALDLPYYGNIAISG
GYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFPLWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVV
VLGARGSDYSSEQMAPMEMAVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family [H]

Homologues:

Organism=Escherichia coli, GI1787680, Length=182, Percent_Identity=34.6153846153846, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680
- InterPro:   IPR023048 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 25536; Mature: 25405

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLFSSLFGKKEENQKVEENKTMSKVLFVKANDRPAEQAVSSKMYETFVTTYKEANPNTE
CCCHHHHHCCCCCCCHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFP
EEEEEEEEECCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEE
LWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEM
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
AVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GLFSSLFGKKEENQKVEENKTMSKVLFVKANDRPAEQAVSSKMYETFVTTYKEANPNTE
CCHHHHHCCCCCCCHHHHHCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ITELDLFALDLPYYGNIAISGGYKRSQGMELTAEEEKAVATVDQYLNQFLEADKVVFAFP
EEEEEEEEECCCCCCCEEEECCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCEEEEEE
LWNFTVPAPLITYISYLSQAGKTFKYTANGPEGLAGGKKVVVLGARGSDYSSEQMAPMEM
CCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHH
AVNYVTTVLGFWGITNPETVVIEGHNQYPDRSQQIVEEGLENVKKVAAKF
HHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]