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The map label for this gene is ilvA [H]

Identifier: 49184731

GI number: 49184731

Start: 1736761

End: 1738023

Strand: Direct

Name: ilvA [H]

Synonym: BAS1718

Alternate gene names: 49184731

Gene position: 1736761-1738023 (Clockwise)

Preceding gene: 49184730

Following gene: 49184733

Centisome position: 33.22

GC content: 35.87

Gene sequence:

>1263_bases
ATGGTGCAAAAGGTAAAGGAGAGAGTGAAAATTGAAGATATCTTAATGGCCCATACTTGTATGAAAGATATCGTTATTAA
AACACCGTTACAACGTGATACAGTTTTATCTGAGAAATATGATTGTGACGTTTATGTAAAACGCGAAGACTTACAATTAA
TTCGCTCTTTCAAAATTCGTGGTGCGTACAATTTAATTCGAAGTTTATCGAAAGAACAATTACAAAACGGTGTTGTTTGT
GCAAGCGCTGGTAATCATGCACAAGGGGTAGCTTATACGTGTAATTTATTGAAGATTCCGTCAAAAATTTTCATGCCAAC
AACAACACCTAAGCAAAAAGTATCACAAGTTCAATTTTTCGGTGGTGATTTCGCAGAGATTGTATTGGTTGGTGATACAT
TCGATAGCTCTTTTCAAGAAGCGCAGCGTTATTGTGAAGAAAATAGAATGACATTTGTTCATCCGTTTGATGATCCGTAC
GTAGTTGCTGGTCAAGGGACAGTGGCAGTTGAAATTATGCATGATATGGAGAAACCGGTTGATTATATCTTTACAGCAAT
CGGCGGTGGTGGATTAGCATCAGGTGTTGGTACATATGTGAAAGGTGTTAGTCCTGCTACACAGGTCATTGGGGTAGAGC
CAATGGGAGCTGCATCTATGAAAGAGGCTTTTCTTCAAAATGATAATGTGGCATTGGAGAAAATAGATAGTTTTGTTGAT
GGGGCAGCGGTTAAAAAGGTAGGAAAGTTAACATTTGAAACTTGTAAAGATGTAATTGATGACATTGTTTTAGTACCAGA
GGGAAAGGTTTGTACGACGATTTTAGAACTGTATAAGAAAAATGCGATTGTAGCTGAACCAGCTGGTGCACTCTCTATTG
CAGCGCTTGATTTATACAGAGATGAAATTAAAGGTAAAACAGTTGTATGCACATTAAGTGGTGGAAACAATGATATTGAT
AGAATGCAAGAAATGAAAGAACGATCTCTTATTTACGAAGGGTTAAAGCATTATTTCATCATTGAATTCCCGCAACGTTC
AGGTGCGTTAAGAGAATTTCTTGATAAAGGATTAGGGCCAGAAGATGATATTACTCGCTTTGAGTATATTAAGAAACATA
ATAAGGAAAATGGTCCAGCATTAGTCGGAGTAGAGTTAAAACATAAAGAAGATTACGAGCAGTTAATTATCCGTTTTAAA
GAAAATAATATTCAATTTATGGAGCTTAATAAAAATCCTGTTTTGTTTGATTTGCTTATTTAA

Upstream 100 bases:

>100_bases
GGACGTTATGCACAAATGGTAACATCGGCGAATACAGGTGCAGTCCTAAAAATCCCGAATTTTGATTGAGTCAATATAAA
AGAGATAAGGATGATATGAA

Downstream 100 bases:

>100_bases
TAGAAGGAAAGAGTTAGCACCCAAATAAAAAAGATAGATACAAGCGAGGAAATACAATGCTTGTTCTATCTTTTTTATAG
TTAAACTAGATATTCATTAA

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MVQKVKERVKIEDILMAHTCMKDIVIKTPLQRDTVLSEKYDCDVYVKREDLQLIRSFKIRGAYNLIRSLSKEQLQNGVVC
ASAGNHAQGVAYTCNLLKIPSKIFMPTTTPKQKVSQVQFFGGDFAEIVLVGDTFDSSFQEAQRYCEENRMTFVHPFDDPY
VVAGQGTVAVEIMHDMEKPVDYIFTAIGGGGLASGVGTYVKGVSPATQVIGVEPMGAASMKEAFLQNDNVALEKIDSFVD
GAAVKKVGKLTFETCKDVIDDIVLVPEGKVCTTILELYKKNAIVAEPAGALSIAALDLYRDEIKGKTVVCTLSGGNNDID
RMQEMKERSLIYEGLKHYFIIEFPQRSGALREFLDKGLGPEDDITRFEYIKKHNKENGPALVGVELKHKEDYEQLIIRFK
ENNIQFMELNKNPVLFDLLI

Sequences:

>Translated_420_residues
MVQKVKERVKIEDILMAHTCMKDIVIKTPLQRDTVLSEKYDCDVYVKREDLQLIRSFKIRGAYNLIRSLSKEQLQNGVVC
ASAGNHAQGVAYTCNLLKIPSKIFMPTTTPKQKVSQVQFFGGDFAEIVLVGDTFDSSFQEAQRYCEENRMTFVHPFDDPY
VVAGQGTVAVEIMHDMEKPVDYIFTAIGGGGLASGVGTYVKGVSPATQVIGVEPMGAASMKEAFLQNDNVALEKIDSFVD
GAAVKKVGKLTFETCKDVIDDIVLVPEGKVCTTILELYKKNAIVAEPAGALSIAALDLYRDEIKGKTVVCTLSGGNNDID
RMQEMKERSLIYEGLKHYFIIEFPQRSGALREFLDKGLGPEDDITRFEYIKKHNKENGPALVGVELKHKEDYEQLIIRFK
ENNIQFMELNKNPVLFDLLI
>Mature_420_residues
MVQKVKERVKIEDILMAHTCMKDIVIKTPLQRDTVLSEKYDCDVYVKREDLQLIRSFKIRGAYNLIRSLSKEQLQNGVVC
ASAGNHAQGVAYTCNLLKIPSKIFMPTTTPKQKVSQVQFFGGDFAEIVLVGDTFDSSFQEAQRYCEENRMTFVHPFDDPY
VVAGQGTVAVEIMHDMEKPVDYIFTAIGGGGLASGVGTYVKGVSPATQVIGVEPMGAASMKEAFLQNDNVALEKIDSFVD
GAAVKKVGKLTFETCKDVIDDIVLVPEGKVCTTILELYKKNAIVAEPAGALSIAALDLYRDEIKGKTVVCTLSGGNNDID
RMQEMKERSLIYEGLKHYFIIEFPQRSGALREFLDKGLGPEDDITRFEYIKKHNKENGPALVGVELKHKEDYEQLIIRFK
ENNIQFMELNKNPVLFDLLI

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia [H]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI11345492, Length=321, Percent_Identity=31.7757009345794, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI33469958, Length=318, Percent_Identity=26.1006289308176, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI19923959, Length=318, Percent_Identity=28.9308176100629, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790207, Length=362, Percent_Identity=37.292817679558, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789505, Length=322, Percent_Identity=37.2670807453416, Blast_Score=185, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI71991565, Length=348, Percent_Identity=33.9080459770115, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17537387, Length=342, Percent_Identity=31.5789473684211, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17508781, Length=279, Percent_Identity=29.3906810035842, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320930, Length=373, Percent_Identity=35.656836461126, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=32.6860841423948, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6319788, Length=301, Percent_Identity=27.2425249169435, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355833, Length=348, Percent_Identity=33.9080459770115, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24645328, Length=250, Percent_Identity=32.8, Blast_Score=101, Evalue=9e-22,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820 [H]

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C [H]

EC number: =4.3.1.19 [H]

Molecular weight: Translated: 46918; Mature: 46918

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQKVKERVKIEDILMAHTCMKDIVIKTPLQRDTVLSEKYDCDVYVKREDLQLIRSFKIR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
GAYNLIRSLSKEQLQNGVVCASAGNHAQGVAYTCNLLKIPSKIFMPTTTPKQKVSQVQFF
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEHEECCCHHCCCCCCCHHHHHHHHHC
GGDFAEIVLVGDTFDSSFQEAQRYCEENRMTFVHPFDDPYVVAGQGTVAVEIMHDMEKPV
CCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCEEEEHHHHHHHCHH
DYIFTAIGGGGLASGVGTYVKGVSPATQVIGVEPMGAASMKEAFLQNDNVALEKIDSFVD
HHHHHHCCCCHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHC
GAAVKKVGKLTFETCKDVIDDIVLVPEGKVCTTILELYKKNAIVAEPAGALSIAALDLYR
CHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
DEIKGKTVVCTLSGGNNDIDRMQEMKERSLIYEGLKHYFIIEFPQRSGALREFLDKGLGP
HHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC
EDDITRFEYIKKHNKENGPALVGVELKHKEDYEQLIIRFKENNIQFMELNKNPVLFDLLI
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHEEEECCCEEEEEECCCCEEEEEEC
>Mature Secondary Structure
MVQKVKERVKIEDILMAHTCMKDIVIKTPLQRDTVLSEKYDCDVYVKREDLQLIRSFKIR
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
GAYNLIRSLSKEQLQNGVVCASAGNHAQGVAYTCNLLKIPSKIFMPTTTPKQKVSQVQFF
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEHEECCCHHCCCCCCCHHHHHHHHHC
GGDFAEIVLVGDTFDSSFQEAQRYCEENRMTFVHPFDDPYVVAGQGTVAVEIMHDMEKPV
CCCEEEEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCEEEECCCEEEEHHHHHHHCHH
DYIFTAIGGGGLASGVGTYVKGVSPATQVIGVEPMGAASMKEAFLQNDNVALEKIDSFVD
HHHHHHCCCCHHHHHHHHHHCCCCCHHHEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHC
GAAVKKVGKLTFETCKDVIDDIVLVPEGKVCTTILELYKKNAIVAEPAGALSIAALDLYR
CHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHH
DEIKGKTVVCTLSGGNNDIDRMQEMKERSLIYEGLKHYFIIEFPQRSGALREFLDKGLGP
HHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCC
EDDITRFEYIKKHNKENGPALVGVELKHKEDYEQLIIRFKENNIQFMELNKNPVLFDLLI
CCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHEEEECCCEEEEEECCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]