Click here to switch to the map view.

The map label for this gene is ansB [H]

Identifier: 49184681

GI number: 49184681

Start: 1682744

End: 1684183

Strand: Direct

Name: ansB [H]

Synonym: BAS1667

Alternate gene names: 49184681

Gene position: 1682744-1684183 (Clockwise)

Preceding gene: 49184680

Following gene: 49184682

Centisome position: 32.18

GC content: 38.61

Gene sequence:

>1440_bases
ATGGCAACATTAACTGAAGTTAAAAATGGTGTTCGAATTGAAAAAGATTTTTTAGGTGAAAAAGAAGTACCAAACTATGC
GTACTACGGCGTACAAACAATGCGTGCAGTAGAAAACTTCCCAATTACAGGATACAAAATCCATGAAGGTTTAATTAAAG
CGTTCGCAATTGTAAAAAAAGCTGCAGCGCTTGCGAATACAGATGTAGGAAGATTGGAATTGAACAAGGGCGGCGCGATC
GCAGAAGCTGCTCAAGAAATTCTTGATGGAAAATGGCATGATCATTTCATCGTAGATCCAATCCAAGGCGGAGCAGGTAC
TTCAATGAACATGAATGCAAATGAAGTCATTGCCAATCGTGCGCTTGAATTATTAGGGATGGAAAAGGGAGACTATCATT
ATATTAGTCCAAATAGTCATGTAAACATGGCACAATCAACAAACGATGCATTCCCAACGGCGATTCATATCGCAACATTA
AACGCATTAGAAGGTTTATTACAAACGATGGGTTATATGCACGATGTATTTGAATTAAAAGCAGAACAGTTCGATCATGT
GATTAAAATGGGGCGTACACATTTACAAGACGCTGTGCCAATTCGTCTTGGACAAGAATTTAAAGCATACTCTCGCGTAC
TTGAACGTGATATGAAACGAATTCAGCAATCGCGTCAACATTTATATGAAGTAAATATGGGAGCAACTGCAGTTGGTACA
GGCTTAAATGCAGATCCAGAATATATTGAAGCAGTTGTAAAACATTTAGCTGCAATTAGTGAACTGCCACTTGTTGGTGC
AGAAGATTTAGTAGATGCAACGCAAAATACGGATGCATACACAGAAGTATCAGCAGCACTTAAAGTATGTATGATGAATA
TGTCTAAAATTGCGAATGACCTTCGCTTAATGGCATCAGGTCCACGTGTTGGCTTAGCGGAAATTATGCTACCTGCTCGT
CAACCAGGTTCTTCTATTATGCCAGGGAAAGTAAACCCTGTTATGCCAGAAGTAATTAATCAAATTGCATTCCAAGTAAT
TGGTAACGACCATACAATTTGTCTTGCTTCAGAAGCAGGTCAATTAGAATTAAACGTGATGGAACCAGTACTTGTTTTCA
ACTTACTACAATCAATTAGCATTATGAATAACGGTTTCCGTGCCTTTACAGATAATTGCTTAAAAGGAATTGAAGCGAAT
GAAGATCGCTTAAAAGAGTATGTTGAGAAGAGTGTAGGAATTATTACAGCCGTGAACCCTCATATCGGTTATGAAGCAGC
AGCTCGCGTTGCGAAAGAAGCAATCGCGACAGGGCAATCCGTTCGAGAACTTTGTGTGAAAAATGGTGTATTGTCACAAG
AAGATTTAGAGTTAATTTTAGATCCATTTGAAATGACACACCCAGGGATTGCAGGAGCAACTCTTTTAAAGAAAAATTAA

Upstream 100 bases:

>100_bases
GAAGTGGGAAGGCGAATTCGATAATGAGAAAGCAAAACAATATGTAGAAACAGTCAAAGAAACAAAAGCAGCATAAGAAA
TCGTTAAGGAGTGAATAATC

Downstream 100 bases:

>100_bases
AAAAAGAGGGGGGACAAGCCCCTCTTTTTTGTTTACAATATAGAAATGCTATATATGAATAGAGGTAGGGATGTTATGAG
CAAGTTTACAGTAGCTTCTA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIKAFAIVKKAAALANTDVGRLELNKGGAI
AEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATL
NALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPAR
QPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEAN
EDRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Sequences:

>Translated_479_residues
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIKAFAIVKKAAALANTDVGRLELNKGGAI
AEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATL
NALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPAR
QPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEAN
EDRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
>Mature_478_residues
ATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIKAFAIVKKAAALANTDVGRLELNKGGAIA
EAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANRALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLN
ALEGLLQTMGYMHDVFELKAEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGTG
LNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIANDLRLMASGPRVGLAEIMLPARQ
PGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAGQLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANE
DRLKEYVEKSVGIITAVNPHIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=464, Percent_Identity=43.75, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI68303547, Length=245, Percent_Identity=29.7959183673469, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI31541964, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI68303542, Length=244, Percent_Identity=29.9180327868852, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=49.6774193548387, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1787896, Length=465, Percent_Identity=42.3655913978495, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17553882, Length=461, Percent_Identity=44.2516268980477, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.2018348623853, Blast_Score=273, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6324993, Length=468, Percent_Identity=41.4529914529915, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640177, Length=463, Percent_Identity=42.7645788336933, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640179, Length=463, Percent_Identity=42.7645788336933, Blast_Score=380, Evalue=1e-105,
Organism=Drosophila melanogaster, GI78710009, Length=462, Percent_Identity=41.7748917748918, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24662684, Length=463, Percent_Identity=42.548596112311, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24583245, Length=463, Percent_Identity=38.6609071274298, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52294; Mature: 52163

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIKAFAIVKK
CCCHHHHHCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAALANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANR
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
ALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELK
HHHHHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIAND
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRLMASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAG
HHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
QLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNP
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECC
HIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
ATLTEVKNGVRIEKDFLGEKEVPNYAYYGVQTMRAVENFPITGYKIHEGLIKAFAIVKK
CCHHHHHCCCEECHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
AAALANTDVGRLELNKGGAIAEAAQEILDGKWHDHFIVDPIQGGAGTSMNMNANEVIANR
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHH
ALELLGMEKGDYHYISPNSHVNMAQSTNDAFPTAIHIATLNALEGLLQTMGYMHDVFELK
HHHHHCCCCCCEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEQFDHVIKMGRTHLQDAVPIRLGQEFKAYSRVLERDMKRIQQSRQHLYEVNMGATAVGT
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GLNADPEYIEAVVKHLAAISELPLVGAEDLVDATQNTDAYTEVSAALKVCMMNMSKIAND
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRLMASGPRVGLAEIMLPARQPGSSIMPGKVNPVMPEVINQIAFQVIGNDHTICLASEAG
HHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCC
QLELNVMEPVLVFNLLQSISIMNNGFRAFTDNCLKGIEANEDRLKEYVEKSVGIITAVNP
CEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECC
HIGYEAAARVAKEAIATGQSVRELCVKNGVLSQEDLELILDPFEMTHPGIAGATLLKKN
CCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCHHHHHHHCCCHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]