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The map label for this gene is ponA [H]

Identifier: 49184475

GI number: 49184475

Start: 1480923

End: 1483613

Strand: Reverse

Name: ponA [H]

Synonym: BAS1458

Alternate gene names: 49184475

Gene position: 1483613-1480923 (Counterclockwise)

Preceding gene: 49184476

Following gene: 49184465

Centisome position: 28.37

GC content: 37.9

Gene sequence:

>2691_bases
ATGTCAGATAATTATCGTTCTCGTACAGAACGAAATCATGTAAAAAATCAAAAGCAAGAAACAAATAAAGAAAAAAAACC
AAAGAAAAAAGGCTCCTTTTTTAAAAAATTCCTTATAGGTTGTCTACTTCTTGGTATCGTTGGTCTTGTAGCTGGCGTTT
CCGCTTTCTTTGTTATGGTGAAGGACGCTCCAAAATTAGACAAATCAAAACTTGTCAATCCTTTATCAACAAAGTTTCTT
GATAAAGACGGGAACTTTTTCTATGAATACGGTGCTGAAAAACGAACCCACGTTACGTATGATCAAATTCCAAAAGTAGT
TGAAAATGCATTCCTTGCAACTGAGGACTCACGTTTCTACGATCATAATGGAATTGATTTTAAACGAACTACAAAAGCAG
TTATGGAAAATGTCACTGGAGGTTTCGGATCTCAAGGTGGTAGTACGATTACACAACAAGTAGTCAAAAACTATTTTCTA
ACGATGGACAAAACTGCAAAGAGAAAAGTACAAGAATGGTACTTATCTTACAAATTAGAGCAACAATACTCTAAACATGA
GATTTTAGAAATGTACTTAAATAAGATTAATTTAGGTAACCGTTCATATGGGATTGCGACAGCAGCGCAAAAATATTATG
GTAAAGATTTGAAAGACTTGCAATTACATGAAGCTGCTATGCTCGCTGGTTTACCCCAAGGTCCAAACATTTACGATCCA
ACGAAAAAGGAAAATGTTGAACGAGCAACAAATCGTCGTAATGTCGTATTGTCATTAATGAATCGACATGGTTATATAAC
AAAAGAAGAAATGAATAACGCAGTTAAGATCCCAGTAACAGAGGGTCTTCAACCGTCTTCAGAAATAACTGAGATGAAGT
ATCAAGCATTTCTAGATGCGGTTGTAAAAGAAGTTGAAAAAGAATATCCTGATGTAAATATTGGTTCAGATGGTTTAACG
ATTCATACAACACTTGATCAAGATGCTCAAGATTATGCTGAAAAAATTATGGATGGCAATCTTATTAAGTATCCAAACGA
TCAATTCCAAGGATCATTCGTATTTATGGATACACAATCTGGTGAAGTTCGAGCAATTGGAGCTGGGCGTAAAGGAAGTA
AGTCTACTTTCAAAGGTCATAATATGGCCACCGATTTAAAACGCCAAGTCGGTTCAACAATGAAACCAATTTTTGACTAC
GGCCCAGCAATTGAAAATTTACAATGGTCTACATACCATCAATTAAATGACTCAGAGTATACGTATTCCAATGGTAAAAA
GATACGAAATGCAACAAATAGTTACAAAGGTGACGTTTCACTACGTGAAGCTTTGAAAAAGTCATTAAACATCCCAGCTT
TAAAAACAGCCCAAGCGGTTGGTCTTCCTAAGTCACAAGCGTTTGCTGAAGGTTTAGGTATGACATTTAAAGATGGCAAA
ACATTTGAATCAACAGCAATTGGTAGTAACGATAGTTCACCATTACAATTAGCGGGTGCATATGCAGCCTTTGGTAATGG
TGGGAACTACAATAAACCGCACTTCGTAAAAGAAGTTACCTTCCCAGATGGGAAAAAGAAAAGTTTTAAACCGAAAGAAC
AACGTGCGATGCAAGACTACACAGCTTACATGGTGACTGACGTTCTTCGTGATGTCGTAAAACCTGGTTCTGGCGGTACT
GGTCCAACAGCATACGTTTCAGGTGTTGATGTTGCCGGTAAAACAGGAACACAAAACTTTGATGCAGACGTTATAAAAGA
ATATGGTATTCCAGCTGATGCAAACAGAGATAGCTGGTTCGCTGGATATACACCGCAATATACAATGGCAGTATGGACTG
GGTACGAAAAAAATGGCCCAGAAAACTACATTAGTGATCGTTATACTAGAATTGCACAGCAAATGTTCCAAGTAATGATG
AGCAAATTCGCTACAGATAAAACTCGTTTCGAACGCCCTTCTTCTGTACAAGAAATAAACGGAGAGTTATATGTAAAAGG
TGCGAAGAAAGATGCAATTAAACAAATTAAAGTAGATGCACCTAGTGGTCTTAATGTCACTTTCGATGGCGCTAGCACAG
TTACACTAAACTGGTCTGGACCAACAGAAGTTGATGCGTATGCAGCAAGCTATAAAGCAACTGACGGTTCAAGTGGTAGT
TTATCAGTAAAAGGTACAACAGCTACTCTTGGTGGTATTAAACCAGGCGTTACTTACAGCTTCTCTGTAGTAGCGAAAAA
AGGTACTGGAACAAGCCCTGCAGTTGGAGCATCCTTCACTGCGCCTGGCGGAACTCCAGACGCGAAGAAAGCCGAGGAAG
AAGCTAAGAAGAAGGCTGAAGAAGAAGCTAAGAAAAAAGCTGATGAGGAAGCTCAGAAAAAGGCCAATGAAGATAAACTA
AAACAAGAAGAAGCTACTAAAAAAGCTGAAGAAGAAGCTAAGAAACAACAAGAACAACAACGTAAACAGCAAGAAGAAGC
TCAAAAGAAAGCTGAAGAGGAAGCTAGAAAAAAAGCTGAAGAAGAAGCTAAGAAACAACAAGAACAACAACGTAAACAGC
AAGAAGAAGCTCAAAAGAAAGCTGAAGAAGAAGCTAAAAAACAACAAGAGCAACAGCAGCAAGAACAACAACAAAATACC
GGAGGAAATACACCTCACGCAGACGGAAATTTTGTTACAACAGAGTCTTAA

Upstream 100 bases:

>100_bases
ATATCCGTGTACTAGACACGGTTTATTTTTCGTGATAGAGTCATCACTAAGGCTCTTTTTTCGACTTTTGGGGAGAGAAT
GAAAGGTAGGAGAAAGTATA

Downstream 100 bases:

>100_bases
TACAAAAAAAGAAGTCCTTTCTCTTAACGAGAACAGGACTTCTTTTTTGTTATCATTTTCTTTACATATAGCTTTTCTAT
TTCAATATACAACTGTGCTA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 896; Mature: 895

Protein sequence:

>896_residues
MSDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFL
DKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGT
GPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMM
SKFATDKTRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPTEVDAYAASYKATDGSSGS
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEEAQKKANEDKL
KQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEQQRKQQEEAQKKAEEEAKKQQEQQQQEQQQNT
GGNTPHADGNFVTTES

Sequences:

>Translated_896_residues
MSDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFL
DKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGT
GPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMM
SKFATDKTRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPTEVDAYAASYKATDGSSGS
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEEAQKKANEDKL
KQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEQQRKQQEEAQKKAEEEAKKQQEQQQQEQQQNT
GGNTPHADGNFVTTES
>Mature_895_residues
SDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFLD
KDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLT
MDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDPT
KKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLTI
HTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDYG
PAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGKT
FESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGTG
PTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMMS
KFATDKTRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPTEVDAYAASYKATDGSSGSL
SVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEEAQKKANEDKLK
QEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEQQRKQQEEAQKKAEEEAKKQQEQQQQEQQQNTG
GNTPHADGNFVTTES

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=548, Percent_Identity=31.021897810219, Blast_Score=217, Evalue=3e-57,
Organism=Escherichia coli, GI87082258, Length=318, Percent_Identity=38.3647798742138, Blast_Score=210, Evalue=3e-55,
Organism=Escherichia coli, GI1788867, Length=603, Percent_Identity=27.0315091210614, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1789601, Length=204, Percent_Identity=32.843137254902, Blast_Score=90, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 99532; Mature: 99401

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLIGCLLLGIVGLVAGVSAFFVMV
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDAPKLDKSKLVNPLSTKFLDKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFY
CCCCCCCHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLTMDKTAKRKVQEWYLSYKLE
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTEGLQPSSEITEMKYQAFLDA
CHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VVKEVEKEYPDVNIGSDGLTIHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQS
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCC
GEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDYGPAIENLQWSTYHQLNDSEY
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHCCCCCCE
TYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
EECCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCCEECCCC
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDY
CCHHHCCCCCCCCCCEEHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
TAYMVTDVLRDVVKPGSGGTGPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWF
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEE
AGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMMSKFATDKTRFERPSSVQEIN
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCC
GELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPTEVDAYAASYKATDGSSGS
CEEEEECCCHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCE
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAE
EEEECCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EEAKKKADEEAQKKANEDKLKQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKKQQEQQRKQQEEAQKKAEEEAKKQQEQQQQEQQQNTGGNTPHADGNFVTTES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]