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The map label for this gene is braB [H]

Identifier: 49184367

GI number: 49184367

Start: 1381185

End: 1382540

Strand: Reverse

Name: braB [H]

Synonym: BAS1350

Alternate gene names: 49184367

Gene position: 1382540-1381185 (Counterclockwise)

Preceding gene: 49184369

Following gene: 49184356

Centisome position: 26.44

GC content: 37.83

Gene sequence:

>1356_bases
ATGAACACTGTATCAAAAAAACATATTTTTTTCACGGGTCTTATGCTATTTTCTTTATTTTTTGGGGCAGGTAATTTAAT
TTTCCCTCCCATGCTTGGACAAAATGCAGGTGAAAACTTTTGGCCAGCAATGATTGGATTTTTACTAACAGGAGTCGGTT
TACCACTACTAACTGTTATCGCAATTTCTTTATCAGGAAATGGTATGCAACAACTTGCAAGTCATGTTCACCCATTATTC
GGAATATTCTTTACGGTAGTTGTATACATTGCCATTGGTCCTTCTATGGGTATTCCACGCGTTGCAAATGTCGCTTATGA
AATGGGCGTAAGTTCTTTCTTACCAGAAACAATCCGTTCTAGCAGCCTAGTGCTATTTGTATATACAATCATATTCTTTG
CTATCGTATTTTGGCTGAGCTTGAATCCATCTAAACTTGTAGATCGCATTGGAAATATATTAACACCTATTTTATTACTC
TCTATTTTCTTATTGTTTGTTAAAAGTGTATTTACACCACTCGGGCAATCAGGTCCAGCGATGCAAGAGTATCAAACTTT
CCCGATCTTCAAAGGATTTATGGAAGGATACTTAACGATGGATACCATTTCAGCACTTGCATTTGGAATTATCGTTGTAA
ATGCTATTCGCTCTAAAGGTGTGAATGATCGTAAATCCATTGCCATCGCAACAGCAAAAGCAGGGCTTATTGCCGCTACT
GGTCTCGTACTTGTATATGGTGCGCTTGGCTGGCTCGGTACAACTAGCGTCTCGCTCGGATATGCAAAAAATGGCGGACA
GTTACTTACTGTTATCGTACAACAATTATTCGGGCCATACGGCTTACTTCTACTAGCTCTAATCGTTACACTCGCTTGCT
TAACGACATGTGTTGGACTCGTATCTGCATGTAGTCAATATTTCTCAACATTATTGACTAAATTTTCATACAAAACATTT
GCAAGTATCATTTGTGCTTTAGGGTTACTAGTTGCTAACTTAGGTTTAACAAAAATCATTGCCATCTCTGTTCCAATTCT
ACTTGTTGTATATCCAATTGCAATCGTACTTGTTCTACTATCGTTACTGCATAAATACTTCGGGGGATATCGTTCTGTTT
ATGTAGGTGCTTTAATTGGAGCAGCTATAGTGAGCGTATTTGATGGATTAAAACAAGGGGGTATTTCAGTTTCATTTATC
ACATCATATTTTGAATTTATCCCTTTATATAATGAAGGAATAGGCTGGCTAGTACCTGCATTAACCGGAGCTCTTATCGG
CCTTACAATCGCTAAACTAAGCGGTGCGAAAAAAGTTCCTTTACTAACCGAATCTCGTGAAGTAAAAGCATCCTAA

Upstream 100 bases:

>100_bases
ATAAATTAGGGTGGTATCGCGGGTAAATATAACTCGTCCCTTTCTTTAGGGACGAGTTTTTTGTGTTCTTACAAATGAAT
GTAAAAAAGGAGGAAAAACA

Downstream 100 bases:

>100_bases
AAAAACTCCCAAAAGGGAGTTTTTTACTTTTGATAAGCAGTATGATTAAAACTGCTTATTTTATACACTTTCGTATAATA
GTCGATATTTGTATCCACTG

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTFPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALTGALIGLTIAKLSGAKKVPLLTESREVKAS

Sequences:

>Translated_451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTFPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALTGALIGLTIAKLSGAKKVPLLTESREVKAS
>Mature_451_residues
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVIAISLSGNGMQQLASHVHPLF
GIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRSSSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLL
SIFLLFVKSVFTPLGQSGPAMQEYQTFPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSIAIATAKAGLIAAT
GLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGLVSACSQYFSTLLTKFSYKTF
ASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLLSLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFI
TSYFEFIPLYNEGIGWLVPALTGALIGLTIAKLSGAKKVPLLTESREVKAS

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=437, Percent_Identity=37.9862700228833, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 48076; Mature: 48076

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHC
SSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLLSIFLLFVKSVFTPLGQSGPA
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQEYQTFPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSIAIATAKAGLIAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHH
GLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGL
HHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSACSQYFSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFIPLYNEGIGWLVPA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LTGALIGLTIAKLSGAKKVPLLTESREVKAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNTVSKKHIFFTGLMLFSLFFGAGNLIFPPMLGQNAGENFWPAMIGFLLTGVGLPLLTVI
CCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AISLSGNGMQQLASHVHPLFGIFFTVVVYIAIGPSMGIPRVANVAYEMGVSSFLPETIRS
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCHHHHHC
SSLVLFVYTIIFFAIVFWLSLNPSKLVDRIGNILTPILLLSIFLLFVKSVFTPLGQSGPA
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
MQEYQTFPIFKGFMEGYLTMDTISALAFGIIVVNAIRSKGVNDRKSIAIATAKAGLIAAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEHHHHHHHHHHH
GLVLVYGALGWLGTTSVSLGYAKNGGQLLTVIVQQLFGPYGLLLLALIVTLACLTTCVGL
HHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
VSACSQYFSTLLTKFSYKTFASIICALGLLVANLGLTKIIAISVPILLVVYPIAIVLVLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLHKYFGGYRSVYVGALIGAAIVSVFDGLKQGGISVSFITSYFEFIPLYNEGIGWLVPA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHH
LTGALIGLTIAKLSGAKKVPLLTESREVKAS
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]