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The map label for this gene is trpA

Identifier: 49184181

GI number: 49184181

Start: 1208712

End: 1209488

Strand: Direct

Name: trpA

Synonym: BAS1162

Alternate gene names: 49184181

Gene position: 1208712-1209488 (Clockwise)

Preceding gene: 49184180

Following gene: 49184182

Centisome position: 23.12

GC content: 39.9

Gene sequence:

>777_bases
ATGGGAGTAGAAAGAATTAAAGCAGCATTTGAAAATGGTAAGAAAGCATTTATTCCGTACGTAATGGGCGGAGATGGTGG
CCTTGAAATATTAAAAGAAAGAATTCGTTTTCTAGACGAAGCGGGAGCAAGCATCGTTGAAATTGGTATCCCGTTTTCAG
ACCCAGTCGCAGATGGCCCAACGATTCAAAGAGCAGGGAAACGAGCGCTAGATAGCGGTGTAACAGTAAAAGGCATTTTT
CAAGCATTAATAGAGGTAAGGAAAGAAGTGCAAATTCCGTTTGTACTCATGACATATTTAAATCCAGTACTTGCATTCGG
GAAAGAACGATTTATCGAGAACTGTATGGAAGCAGGTGTAGACGGCATCATCGTTCCAGACTTACCGTATGAAGAACAAG
ACATTATTGCTCCGTTACTTCGAGAGGCAAACATTGCGCTCATTCCACTTGTTACTGTTACGAGCCCAATTGAGCGAATC
AAAAAAATTACGAGTGAATCAGAAGGATTTGTCTACGCCGTTACAGTAGCGGGCGTAACAGGAGTACGTCAAAATTTTAA
AGATGAGATTCATAGTTACTTAGAAAAAGTGAAATCGCATACACATTTACCGGTAGTGGCAGGGTTCGGTATTTCAACAA
AGGAACATGTGGAAGAAATGGTTACAATATGTGATGGTGTTGTCGTTGGAAGTAAAGTGATTGAGCTATTAGAAAATGAA
AAACGAGAAGAAATATGTGAATTCATACAGGCAACAAAACAAAAAGAAGAGGCGTAA

Upstream 100 bases:

>100_bases
GCCGCAAATGAAGGAAGATGAAGGACTTGTCATTTGTTTATCCGGCCGTGGTGATAAAGATGTAGAGAGTATAAAACGTT
ACATGGAAGAGGTGTAAAAA

Downstream 100 bases:

>100_bases
GTCTCTTCTTTTTGTTTTGTGTAATAAATGTTAAAATATACAGAAAATACATATAATTTAGAGGGTGAGAAAGCATGCTA
AGACGAAAACTACTATATCT

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MGVERIKAAFENGKKAFIPYVMGGDGGLEILKERIRFLDEAGASIVEIGIPFSDPVADGPTIQRAGKRALDSGVTVKGIF
QALIEVRKEVQIPFVLMTYLNPVLAFGKERFIENCMEAGVDGIIVPDLPYEEQDIIAPLLREANIALIPLVTVTSPIERI
KKITSESEGFVYAVTVAGVTGVRQNFKDEIHSYLEKVKSHTHLPVVAGFGISTKEHVEEMVTICDGVVVGSKVIELLENE
KREEICEFIQATKQKEEA

Sequences:

>Translated_258_residues
MGVERIKAAFENGKKAFIPYVMGGDGGLEILKERIRFLDEAGASIVEIGIPFSDPVADGPTIQRAGKRALDSGVTVKGIF
QALIEVRKEVQIPFVLMTYLNPVLAFGKERFIENCMEAGVDGIIVPDLPYEEQDIIAPLLREANIALIPLVTVTSPIERI
KKITSESEGFVYAVTVAGVTGVRQNFKDEIHSYLEKVKSHTHLPVVAGFGISTKEHVEEMVTICDGVVVGSKVIELLENE
KREEICEFIQATKQKEEA
>Mature_257_residues
GVERIKAAFENGKKAFIPYVMGGDGGLEILKERIRFLDEAGASIVEIGIPFSDPVADGPTIQRAGKRALDSGVTVKGIFQ
ALIEVRKEVQIPFVLMTYLNPVLAFGKERFIENCMEAGVDGIIVPDLPYEEQDIIAPLLREANIALIPLVTVTSPIERIK
KITSESEGFVYAVTVAGVTGVRQNFKDEIHSYLEKVKSHTHLPVVAGFGISTKEHVEEMVTICDGVVVGSKVIELLENEK
REEICEFIQATKQKEEA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=250, Percent_Identity=35.2, Blast_Score=134, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6321412, Length=258, Percent_Identity=34.4961240310078, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_BACAA (C3P3U1)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002865770.1
- ProteinModelPortal:   C3P3U1
- SMR:   C3P3U1
- EnsemblBacteria:   EBBACT00000128123
- GeneID:   7853307
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_1330
- GeneTree:   EBGT00050000001942
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28355; Mature: 28224

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 47-47 ACT_SITE 58-58

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVERIKAAFENGKKAFIPYVMGGDGGLEILKERIRFLDEAGASIVEIGIPFSDPVADGP
CCHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
TIQRAGKRALDSGVTVKGIFQALIEVRKEVQIPFVLMTYLNPVLAFGKERFIENCMEAGV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGIIVPDLPYEEQDIIAPLLREANIALIPLVTVTSPIERIKKITSESEGFVYAVTVAGVT
CEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHH
GVRQNFKDEIHSYLEKVKSHTHLPVVAGFGISTKEHVEEMVTICDGVVVGSKVIELLENE
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KREEICEFIQATKQKEEA
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GVERIKAAFENGKKAFIPYVMGGDGGLEILKERIRFLDEAGASIVEIGIPFSDPVADGP
CHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
TIQRAGKRALDSGVTVKGIFQALIEVRKEVQIPFVLMTYLNPVLAFGKERFIENCMEAGV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DGIIVPDLPYEEQDIIAPLLREANIALIPLVTVTSPIERIKKITSESEGFVYAVTVAGVT
CEEEECCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEEEHHHH
GVRQNFKDEIHSYLEKVKSHTHLPVVAGFGISTKEHVEEMVTICDGVVVGSKVIELLENE
HHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
KREEICEFIQATKQKEEA
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA