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The map label for this gene is scrR [H]

Identifier: 49183743

GI number: 49183743

Start: 778048

End: 779034

Strand: Reverse

Name: scrR [H]

Synonym: BAS0719

Alternate gene names: 49183743

Gene position: 779034-778048 (Counterclockwise)

Preceding gene: 49183744

Following gene: 49183742

Centisome position: 14.9

GC content: 32.93

Gene sequence:

>987_bases
ATGACTAAAACAATTGCAGATATCGCCAAATTGGCTGGAGTTGCAAAAAGCACAGTTTCTCGTTACTTAAACGGGGGATA
CGTAAGTGATAAAACAAAACTTAAAATCGAAAGTATTATTCAAGAAACAAATTTTTCTCCCAATACATTTGCTCAAAGCT
TAAAAGCAAAAACAACAAATTTAATTGGTGTTATTATCCCCCGATTAGACTCCTTCGCTACAATGAAAACACTTATCGGG
ATTGATAATACGTTACAGGAAAATAACTATCAAATGCTTGTAGCAAATGCAAATCAAACAATTGAAACTGAAATACAAGC
AATGGAAAACTTTATAAAACAAAGAGTAGCTGGCATTATTTTATTAACTAAAACTTTAACAAATAAACATCAACAAATTA
TTGCTAATTCAAATATCCCTATTTTATTCGTTGGGCAAGAATATAAAGATCAATATTGCCTCGTTCATGATGATTATGAT
GCAGCTTATGAGTTAGGAGCATACGTGTTATCACAAGGGCATAGAAATATCGCCTATTTAGGAGTAGAAAAAGATGATAT
TTCTGTTGGTATAAATCGAAAAAACGGTTTTCAAAAAGCCATTGAAAATTTAGAACCAACTTGCAGTGTTTGTTATTACG
AAACATCTTTCCATATAGAAGATGCCATGAAACAAGTACAACATATTTTAGATAATAATCGCCCAACTCTTATCGTATGT
GCTACAGATAACATCGCTCTTGGCGCAATGAAAGTTATTCACTCCCACTCCCTTTCTGTACCAGGTGATATTTCTGTAAC
AGGATTTGGGGGATATGATATTTCAGAAATGGTACACCCTAGCTTAACGACAATTGCATTTGATTATGAGTATGCTGGAA
AGCTTGCTGCCACTTCTCTTTCACAGCTTGTTGAAAACAAAACTATACCAAAAATATTACACTCTCGTTATACATTAAAA
ATCCAAGAAAGCGTTGACAAAATATAA

Upstream 100 bases:

>100_bases
ATACGTGAGGTGGTTTTTTCTTTTCCTCTTCTTGTTTACGTCCTCCCCAATTCTCCCTATAATAAAATTAATATTTTTAT
GAATACGAAGGTGAAATAAT

Downstream 100 bases:

>100_bases
AGGCACTCTATAATAAAAATAAAACCGGTTCCACATTAAAAACCAGGATTCTTCACATCAATTCCCTGTTTTTTATTTTA
ATCGTTCACGGAACCGGTTC

Product: sucrose operon repressor

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTKTIADIAKLAGVAKSTVSRYLNGGYVSDKTKLKIESIIQETNFSPNTFAQSLKAKTTNLIGVIIPRLDSFATMKTLIG
IDNTLQENNYQMLVANANQTIETEIQAMENFIKQRVAGIILLTKTLTNKHQQIIANSNIPILFVGQEYKDQYCLVHDDYD
AAYELGAYVLSQGHRNIAYLGVEKDDISVGINRKNGFQKAIENLEPTCSVCYYETSFHIEDAMKQVQHILDNNRPTLIVC
ATDNIALGAMKVIHSHSLSVPGDISVTGFGGYDISEMVHPSLTTIAFDYEYAGKLAATSLSQLVENKTIPKILHSRYTLK
IQESVDKI

Sequences:

>Translated_328_residues
MTKTIADIAKLAGVAKSTVSRYLNGGYVSDKTKLKIESIIQETNFSPNTFAQSLKAKTTNLIGVIIPRLDSFATMKTLIG
IDNTLQENNYQMLVANANQTIETEIQAMENFIKQRVAGIILLTKTLTNKHQQIIANSNIPILFVGQEYKDQYCLVHDDYD
AAYELGAYVLSQGHRNIAYLGVEKDDISVGINRKNGFQKAIENLEPTCSVCYYETSFHIEDAMKQVQHILDNNRPTLIVC
ATDNIALGAMKVIHSHSLSVPGDISVTGFGGYDISEMVHPSLTTIAFDYEYAGKLAATSLSQLVENKTIPKILHSRYTLK
IQESVDKI
>Mature_327_residues
TKTIADIAKLAGVAKSTVSRYLNGGYVSDKTKLKIESIIQETNFSPNTFAQSLKAKTTNLIGVIIPRLDSFATMKTLIGI
DNTLQENNYQMLVANANQTIETEIQAMENFIKQRVAGIILLTKTLTNKHQQIIANSNIPILFVGQEYKDQYCLVHDDYDA
AYELGAYVLSQGHRNIAYLGVEKDDISVGINRKNGFQKAIENLEPTCSVCYYETSFHIEDAMKQVQHILDNNRPTLIVCA
TDNIALGAMKVIHSHSLSVPGDISVTGFGGYDISEMVHPSLTTIAFDYEYAGKLAATSLSQLVENKTIPKILHSRYTLKI
QESVDKI

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=336, Percent_Identity=27.9761904761905, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=26.2820512820513, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=30.8681672025724, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786540, Length=319, Percent_Identity=29.4670846394984, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=25.2396166134185, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=25.9259259259259, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=319, Percent_Identity=26.6457680250784, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790369, Length=290, Percent_Identity=28.2758620689655, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790194, Length=325, Percent_Identity=26.4615384615385, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788474, Length=321, Percent_Identity=26.4797507788162, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786268, Length=290, Percent_Identity=26.8965517241379, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787580, Length=328, Percent_Identity=25, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787906, Length=254, Percent_Identity=25.9842519685039, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36360; Mature: 36229

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTIADIAKLAGVAKSTVSRYLNGGYVSDKTKLKIESIIQETNFSPNTFAQSLKAKTTN
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LIGVIIPRLDSFATMKTLIGIDNTLQENNYQMLVANANQTIETEIQAMENFIKQRVAGII
HEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
LLTKTLTNKHQQIIANSNIPILFVGQEYKDQYCLVHDDYDAAYELGAYVLSQGHRNIAYL
EEEHHHCCHHHHEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
GVEKDDISVGINRKNGFQKAIENLEPTCSVCYYETSFHIEDAMKQVQHILDNNRPTLIVC
EEECCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
ATDNIALGAMKVIHSHSLSVPGDISVTGFGGYDISEMVHPSLTTIAFDYEYAGKLAATSL
ECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCCCCEEEEEEHHHHCHHHHHHH
SQLVENKTIPKILHSRYTLKIQESVDKI
HHHHHCCCHHHHHHCCEEEEEHHHHCCC
>Mature Secondary Structure 
TKTIADIAKLAGVAKSTVSRYLNGGYVSDKTKLKIESIIQETNFSPNTFAQSLKAKTTN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LIGVIIPRLDSFATMKTLIGIDNTLQENNYQMLVANANQTIETEIQAMENFIKQRVAGII
HEEEEECCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
LLTKTLTNKHQQIIANSNIPILFVGQEYKDQYCLVHDDYDAAYELGAYVLSQGHRNIAYL
EEEHHHCCHHHHEEECCCCCEEEECCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
GVEKDDISVGINRKNGFQKAIENLEPTCSVCYYETSFHIEDAMKQVQHILDNNRPTLIVC
EEECCCEEEEECCCCHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
ATDNIALGAMKVIHSHSLSVPGDISVTGFGGYDISEMVHPSLTTIAFDYEYAGKLAATSL
ECCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHCCCCCEEEEEEHHHHCHHHHHHH
SQLVENKTIPKILHSRYTLKIQESVDKI
HHHHHCCCHHHHHHCCEEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550467 [H]