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The map label for this gene is sacP [H]

Identifier: 49183742

GI number: 49183742

Start: 776543

End: 777919

Strand: Reverse

Name: sacP [H]

Synonym: BAS0718

Alternate gene names: 49183742

Gene position: 777919-776543 (Counterclockwise)

Preceding gene: 49183743

Following gene: 49183741

Centisome position: 14.88

GC content: 36.46

Gene sequence:

>1377_bases
ATGAATATGAAACAAATTGCAACACAAGTATTACAAAACATCGGTGGAAAGGAAAACATTATGCGCGCTACCCATTGTGC
TACTCGCCTTCGTCTTGTACTAAAAGATGAAACAAAAATAAACGCTTCCGAAATCGAAAACATCGATGAAGTGAAAGGTG
CTTTCGCAACGACCGAACAATATCAAATTATTTTTGGAACTGGCATAGTAAACAAAGTATATGATGAATTCTCAAAACTA
ATTGGGATAGCCGAACTAGATTCTACAAGATCAAACGATATACCTAAGAAAAAAATGAATCCTATCGCCCGCCTAGCAAA
GACACTATCCAATATTTTTGTTCCAATTATTCCAGCTATCGTCGCAAGCGGTTTACTTATGGGTCTACTCGGAATGTTGA
AAGCGTTTAAATTAGTTCCTGGAGATCACTCACTTATCCAATTACTTGATATGTTCTCAAGTGCAGCTTTCATTATTTTA
CCTATTTTAATTGGAGTGAGTGCCGCTAAAGAATTTGGTGGAAATATTTTCTTAGGTGCAGTTGTTGGTGGTATATTAAC
TCACCCAAGCTTAACAAATCCTTGGACTCTTTCAAATGCAAAACCAACTGTTTTACATTTCCTTGGTATGGATATTGATA
TGATTGGCTATCAAGGTACTGTTTTACCTATTTTATTATGCGTATATGTTATGAGTACAATTGAAAAAGAACTTCGAAAA
CGTGTTCCGAACTCTTTAGATTTATTAGTTACACCATTCTTAACAATTATTATAACTGGCTTTTTATCACTTATTATTAT
CGGACCAATTGGATATAAAATCGGTGACGGCATTACGTACCTTTTAAATACAATTTATCAATTTGCAGGACCAGTAGCTG
GACTTATATTCGGCGGATTGTATTCAACAATCGTTCTTACTGGCTTACATCATAGCTTCCACGCTATTGAAGCGGGCTTA
CTTGCAAATAAAGATATCGGTGTCAATTTCTTACTCCCTATTTGGTCAATGGCGAACGTTGCACAGGGCGGTGCTACTTT
AGCAGTTTATTTCAAAACAAAAAACAAAAAAACGAAAGAAATTGCAATTCCAGCAGCAGCTTCAGCATTTTTAGGAATTA
CGGAACCAGCTATTTTTGGTGTCAATTTAAAACTGGGTAAACCATTTATTGCAGCAGCTATTGGCGGCGCAATTGGAGGC
GCTTACGTTGTCTGGATGCAAGTAGCAGCTAACGCATACGGTTTAACAGGTATTCCGATGCTTACAATTGTTGCACCTCT
TGGCATGATGAACATGATTCACTATATAATTGGCTTCGCTATTGCAGTTACCACTGCTTTTGTTGCCACATTCATTTTAT
ATAAAGAAAGTAAATAA

Upstream 100 bases:

>100_bases
TTCCACATTAAAAACCAGGATTCTTCACATCAATTCCCTGTTTTTTATTTTAATCGTTCACGGAACCGGTTCCATAAAAA
TATAGTAAGGGGGAATTTTT

Downstream 100 bases:

>100_bases
TAACGGGGTGAAAACCTATGTCAAAATATAAAACAATACTGCAATCTAATAAAGACGAATTACATTCTTTATATGAAATT
GCAAACCAAGATTCATGGAA

Product: PTS system sucrose-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIIBC-Scr; EII-Scr; Sucrose-specific phosphotransferase enzyme IIB component; PTS system sucrose-specific EIIB component; Sucrose permease IIC component; PTS system sucrose-specific EIIC component [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNMKQIATQVLQNIGGKENIMRATHCATRLRLVLKDETKINASEIENIDEVKGAFATTEQYQIIFGTGIVNKVYDEFSKL
IGIAELDSTRSNDIPKKKMNPIARLAKTLSNIFVPIIPAIVASGLLMGLLGMLKAFKLVPGDHSLIQLLDMFSSAAFIIL
PILIGVSAAKEFGGNIFLGAVVGGILTHPSLTNPWTLSNAKPTVLHFLGMDIDMIGYQGTVLPILLCVYVMSTIEKELRK
RVPNSLDLLVTPFLTIIITGFLSLIIIGPIGYKIGDGITYLLNTIYQFAGPVAGLIFGGLYSTIVLTGLHHSFHAIEAGL
LANKDIGVNFLLPIWSMANVAQGGATLAVYFKTKNKKTKEIAIPAAASAFLGITEPAIFGVNLKLGKPFIAAAIGGAIGG
AYVVWMQVAANAYGLTGIPMLTIVAPLGMMNMIHYIIGFAIAVTTAFVATFILYKESK

Sequences:

>Translated_458_residues
MNMKQIATQVLQNIGGKENIMRATHCATRLRLVLKDETKINASEIENIDEVKGAFATTEQYQIIFGTGIVNKVYDEFSKL
IGIAELDSTRSNDIPKKKMNPIARLAKTLSNIFVPIIPAIVASGLLMGLLGMLKAFKLVPGDHSLIQLLDMFSSAAFIIL
PILIGVSAAKEFGGNIFLGAVVGGILTHPSLTNPWTLSNAKPTVLHFLGMDIDMIGYQGTVLPILLCVYVMSTIEKELRK
RVPNSLDLLVTPFLTIIITGFLSLIIIGPIGYKIGDGITYLLNTIYQFAGPVAGLIFGGLYSTIVLTGLHHSFHAIEAGL
LANKDIGVNFLLPIWSMANVAQGGATLAVYFKTKNKKTKEIAIPAAASAFLGITEPAIFGVNLKLGKPFIAAAIGGAIGG
AYVVWMQVAANAYGLTGIPMLTIVAPLGMMNMIHYIIGFAIAVTTAFVATFILYKESK
>Mature_458_residues
MNMKQIATQVLQNIGGKENIMRATHCATRLRLVLKDETKINASEIENIDEVKGAFATTEQYQIIFGTGIVNKVYDEFSKL
IGIAELDSTRSNDIPKKKMNPIARLAKTLSNIFVPIIPAIVASGLLMGLLGMLKAFKLVPGDHSLIQLLDMFSSAAFIIL
PILIGVSAAKEFGGNIFLGAVVGGILTHPSLTNPWTLSNAKPTVLHFLGMDIDMIGYQGTVLPILLCVYVMSTIEKELRK
RVPNSLDLLVTPFLTIIITGFLSLIIIGPIGYKIGDGITYLLNTIYQFAGPVAGLIFGGLYSTIVLTGLHHSFHAIEAGL
LANKDIGVNFLLPIWSMANVAQGGATLAVYFKTKNKKTKEIAIPAAASAFLGITEPAIFGVNLKLGKPFIAAAIGGAIGG
AYVVWMQVAANAYGLTGIPMLTIVAPLGMMNMIHYIIGFAIAVTTAFVATFILYKESK

Specific function: Acts as both a kinase and a phosphatase on sacT [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=429, Percent_Identity=32.4009324009324, Blast_Score=238, Evalue=8e-64,
Organism=Escherichia coli, GI1790159, Length=458, Percent_Identity=30.7860262008734, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI48994906, Length=469, Percent_Identity=29.2110874200426, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788769, Length=444, Percent_Identity=29.2792792792793, Blast_Score=179, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010973 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 49084; Mature: 49084

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKQIATQVLQNIGGKENIMRATHCATRLRLVLKDETKINASEIENIDEVKGAFATTEQ
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCCC
YQIIFGTGIVNKVYDEFSKLIGIAELDSTRSNDIPKKKMNPIARLAKTLSNIFVPIIPAI
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMLKAFKLVPGDHSLIQLLDMFSSAAFIILPILIGVSAAKEFGGNIFLGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
VVGGILTHPSLTNPWTLSNAKPTVLHFLGMDIDMIGYQGTVLPILLCVYVMSTIEKELRK
HHHHHHCCCCCCCCEECCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RVPNSLDLLVTPFLTIIITGFLSLIIIGPIGYKIGDGITYLLNTIYQFAGPVAGLIFGGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YSTIVLTGLHHSFHAIEAGLLANKDIGVNFLLPIWSMANVAQGGATLAVYFKTKNKKTKE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCE
IAIPAAASAFLGITEPAIFGVNLKLGKPFIAAAIGGAIGGAYVVWMQVAANAYGLTGIPM
EECCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LTIVAPLGMMNMIHYIIGFAIAVTTAFVATFILYKESK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNMKQIATQVLQNIGGKENIMRATHCATRLRLVLKDETKINASEIENIDEVKGAFATTEQ
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHCCCCC
YQIIFGTGIVNKVYDEFSKLIGIAELDSTRSNDIPKKKMNPIARLAKTLSNIFVPIIPAI
EEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
VASGLLMGLLGMLKAFKLVPGDHSLIQLLDMFSSAAFIILPILIGVSAAKEFGGNIFLGA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHH
VVGGILTHPSLTNPWTLSNAKPTVLHFLGMDIDMIGYQGTVLPILLCVYVMSTIEKELRK
HHHHHHCCCCCCCCEECCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
RVPNSLDLLVTPFLTIIITGFLSLIIIGPIGYKIGDGITYLLNTIYQFAGPVAGLIFGGL
HCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHH
YSTIVLTGLHHSFHAIEAGLLANKDIGVNFLLPIWSMANVAQGGATLAVYFKTKNKKTKE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCE
IAIPAAASAFLGITEPAIFGVNLKLGKPFIAAAIGGAIGGAYVVWMQVAANAYGLTGIPM
EECCHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LTIVAPLGMMNMIHYIIGFAIAVTTAFVATFILYKESK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3122206; 7934828; 9384377; 10568751 [H]