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The map label for this gene is atoC [C]

Identifier: 49183339

GI number: 49183339

Start: 333724

End: 335091

Strand: Direct

Name: atoC [C]

Synonym: BAS0311

Alternate gene names: 49183339

Gene position: 333724-335091 (Clockwise)

Preceding gene: 49183338

Following gene: 49183340

Centisome position: 6.38

GC content: 35.45

Gene sequence:

>1368_bases
ATGGTTGCAGAAAAGGAACGAGTGTTAATGGATTTACAAGATGTATTTGAATATGCGTTTGACGAAATTTTTGTGACTGA
CGACAAGGGAATCGTTGTGCGTGTAAATAGTACATGTGAAAGGCACTATCAAATAGCTGCTAAAGAGTTAGTAGGTAAGC
ATGTAAAAGAATTGCAGAAGGATGGGATCTTTTATCCATCAGCAACGTTAGAAGTAATTGAAAAAAAGAGGCCGATTGAA
CTCGTTCAAACGACAAAATCAGGAGAGTATTTACACGTTCGTACAAGGCCTGTTTTTGATGATGAGGGAAATTTAAGAAG
GGTGATTAGTTACTCTCGTGATCTTACAGAACTCTATCAATTACGTCAAAAGGTAGAAGAAATGGATAATCAGTTAAAAA
CATATAAAAAAGAATTAAGAGAAACATATGAACATGAAGGACTTATTTTTAAAAGTATCGCTATGCAAAAAATAATCGAG
ACAATCAAGAAAGTATCCGTGGTAGATAGTACTGTTCTTGTTTTAGGTGAGACAGGAGTAGGGAAGAGTCGATTGGTGCG
TCATTTACATGAAATGAGTAACCGGAAGAACGAAAGTTTCTATGAAATTAATTGTGCGGCATTGCCAACGAATTTAATTG
AGTCAGAGCTTTTTGGATATTCAGGTGGATCCTTTACAGGTGCGAATCGTGAAGGGAAAAAGGGATTATTAGAATCAGCG
CATAAAGGAACTCTTTTTTTAGATGAAATTGGTGAAATGCCACTTGAAATTCAAGCAAAGCTTTTGCAAGTGTTGCAAGA
GAAAACGTTTCGCCCTATAGGCGGAAGAGAATTAAAAAAGGTGGACGTTAGGATTGTAGCGGCAACAAATAGAGATTTAA
GTATGATGGTGAAACAAGGAACATTCCGGAAAGATTTATATTATCGTCTGAATGTCATTCCAATTTTAATTCCACCACTA
AGGGAAAGAACTGAAGACATTTTGCCACTCATTTATCATTATTTACAACACTTTAACGAGAAATATGGAAGGAATGTAAA
ATTAGCGCCTAGTACATTGCAAATGTTTGTTGGTTACCCGTGGGAAGGAAATAATAGAGAAATTGAAAATGTAATTGAGC
GAATTGTTATAACTGCTGATGATATCGTTACGATAGAGGATTTGCCAATAGCGATGCAAGAATCAACGGTTGAACAATCG
GGCCAAAGTCTCTATAAAATGTTAGAAGAAGTAGAACGGAATATTATTTTGAAAGCATATAAAACATATGGATCGAGTTA
TAAAGTAGCGGAGTTTTTAAAAATAAGTCAATCTGCTGCCACGAGAAAAATAAAGAAACTTATAGAGGAGGAAGAGAACA
TTGGATAA

Upstream 100 bases:

>100_bases
TAGCAAACATTCTAGCTGACTCTATATAAAATGCAGAAAATTCACTTTATAATAAAGGTAGTGAAGTGAATCGATGGATT
GGAAGTTAGGGGTGGCTAGG

Downstream 100 bases:

>100_bases
AAAAGCGACATTTGTAAATGTAGAAAAAAAGGCGATGTATATAAATGGTGAGTGGATTACATTGCAAGAACAAATCGAAG
TAAATAATCCAGCAACGAAG

Product: sensory box sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDDKGIVVRVNSTCERHYQIAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIIE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEMSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPILIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG

Sequences:

>Translated_455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDDKGIVVRVNSTCERHYQIAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIIE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEMSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPILIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
>Mature_455_residues
MVAEKERVLMDLQDVFEYAFDEIFVTDDKGIVVRVNSTCERHYQIAAKELVGKHVKELQKDGIFYPSATLEVIEKKRPIE
LVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQLRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIIE
TIKKVSVVDSTVLVLGETGVGKSRLVRHLHEMSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQGTFRKDLYYRLNVIPILIPPL
RERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYPWEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQS
GQSLYKMLEEVERNIILKAYKTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=351, Percent_Identity=41.3105413105413, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1789233, Length=318, Percent_Identity=40.5660377358491, Blast_Score=241, Evalue=1e-64,
Organism=Escherichia coli, GI1789087, Length=231, Percent_Identity=49.3506493506493, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=48.0519480519481, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1790437, Length=334, Percent_Identity=37.7245508982036, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1790299, Length=328, Percent_Identity=39.6341463414634, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1788905, Length=263, Percent_Identity=41.06463878327, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=35.0819672131148, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1786524, Length=363, Percent_Identity=33.3333333333333, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=42.9752066115703, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081872, Length=237, Percent_Identity=39.2405063291139, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI87081858, Length=463, Percent_Identity=26.5658747300216, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789828, Length=243, Percent_Identity=36.6255144032922, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR002078 [H]

Pfam domain/function: PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52454; Mature: 52454

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAEKERVLMDLQDVFEYAFDEIFVTDDKGIVVRVNSTCERHYQIAAKELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIIETIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEMSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPILIPPLRERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYP
CHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHEECC
WEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVAEKERVLMDLQDVFEYAFDEIFVTDDKGIVVRVNSTCERHYQIAAKELVGKHVKELQK
CCCCHHHHHHHHHHHHHHHHHHEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
DGIFYPSATLEVIEKKRPIELVQTTKSGEYLHVRTRPVFDDEGNLRRVISYSRDLTELYQ
CCCCCCCHHHHHHHCCCCHHHHEECCCCCEEEEECCCCCCCCCCHHHHHHHHCCHHHHHH
LRQKVEEMDNQLKTYKKELRETYEHEGLIFKSIAMQKIIETIKKVSVVDSTVLVLGETGV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
GKSRLVRHLHEMSNRKNESFYEINCAALPTNLIESELFGYSGGSFTGANREGKKGLLESA
CHHHHHHHHHHHHCCCCCCEEEEEEHHCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHC
HKGTLFLDEIGEMPLEIQAKLLQVLQEKTFRPIGGRELKKVDVRIVAATNRDLSMMVKQG
CCCCEEHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCHHHHHHCC
TFRKDLYYRLNVIPILIPPLRERTEDILPLIYHYLQHFNEKYGRNVKLAPSTLQMFVGYP
CHHHHHHHEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHEECC
WEGNNREIENVIERIVITADDIVTIEDLPIAMQESTVEQSGQSLYKMLEEVERNIILKAY
CCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTYGSSYKVAEFLKISQSAATRKIKKLIEEEENIG
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]