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The map label for this gene is dagK [H]

Identifier: 49183336

GI number: 49183336

Start: 330304

End: 331209

Strand: Direct

Name: dagK [H]

Synonym: BAS0308

Alternate gene names: 49183336

Gene position: 330304-331209 (Clockwise)

Preceding gene: 49183335

Following gene: 49183337

Centisome position: 6.32

GC content: 39.74

Gene sequence:

>906_bases
ATGATGAAGCGAGCAAGAATTATTTATAATCCTACTTCTGGGCGTGAGCTATTTAAGAAGAGTTTACCAGAAGTATTACA
AAAATTAGAACAAGCTGGATATGAGGCATCTTGTCATGCGACAACGGGTCCTGGCGACGCAACTGTGGCGGCGAGGCAAG
CTGCGGATCGTAAATTTGATGTTGTTATCGCTGCTGGTGGTGACGGCACGTTAAATGAAGTGGTGAACGGTTTAGTAGGG
CATGAACATCGTCCGAAGTTCGGAATTATTCCGGTTGGAACGACTAATGACTTTGCACGTGCAATCGGTGTACCTCGTTC
TATTGAAGAGGCAGCAGATATTATTTGTGAAGGAAAAACTGTGCCATTAGACCTTGGTAGAGCAAACGATACATATTTTA
TTAACATCGCTGGTGGCGGTCGCATTACAGAATTAACATATGAAGTACCGAGTAAGCTAAAGACTGTATTAGGACAACTT
GCTTATTATTTAAAAGGTATCGAAATGCTACCATCACTACACCCAACATATGTTGAAATTGAGTATGATGGAAAATTACT
ACAAGAAGAAATTACGATGTTTTTAATTACGAATACTCGTTCAGTGGGCGGCTTTGAAAAGGTAGCACCATATGCATCAA
TTAACGATGGCTTATTTGATTTATTAGTGCTGAAAAAAGGTTCGATTGCTGACTTAATTAAAGCAGCAACACAAGCGCAG
CGTGGTGAACATATTAACAATCCAAAAGTACTGTACACACAAGCTAACCGAATTAAAGTGCATTCACCAGACAAACTAAT
GATTAATTTAGATGGTGAATACGGTGGAGATGCACCGATGGAATTCGAAAATATATATCATTGTCTGGAACTATTTGTTC
CTGAACATCAAGAGGATGCCCTGTAA

Upstream 100 bases:

>100_bases
AAAGAAGGATAGGAAGATGAGAGGAGGTAGGCATGGGAATACTGAATAGAGGTTCGGTATGTTCTTTTTCTGTGCATAAA
GAAATTTCCTGTGCTAATAA

Downstream 100 bases:

>100_bases
AGGCATCCTTTTTATTATATAGAGGAATTGTAAATTGGAAGTTAGTTTGATTTTCATGGTATTTTTACAATTCGTTTATT
TTATATCCATACTTTATTGA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MMKRARIIYNPTSGRELFKKSLPEVLQKLEQAGYEASCHATTGPGDATVAARQAADRKFDVVIAAGGDGTLNEVVNGLVG
HEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEHQEDAL

Sequences:

>Translated_301_residues
MMKRARIIYNPTSGRELFKKSLPEVLQKLEQAGYEASCHATTGPGDATVAARQAADRKFDVVIAAGGDGTLNEVVNGLVG
HEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEHQEDAL
>Mature_301_residues
MMKRARIIYNPTSGRELFKKSLPEVLQKLEQAGYEASCHATTGPGDATVAARQAADRKFDVVIAAGGDGTLNEVVNGLVG
HEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKTVPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQL
AYYLKGIEMLPSLHPTYVEIEYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEHQEDAL

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=279, Percent_Identity=32.258064516129, Blast_Score=143, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 32988; Mature: 32988

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKRARIIYNPTSGRELFKKSLPEVLQKLEQAGYEASCHATTGPGDATVAARQAADRKFD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCEEE
VVIAAGGDGTLNEVVNGLVGHEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKT
EEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHEECCCC
VPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEI
CEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
EECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEHQEDA
CCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
L
C
>Mature Secondary Structure
MMKRARIIYNPTSGRELFKKSLPEVLQKLEQAGYEASCHATTGPGDATVAARQAADRKFD
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHCCCEEE
VVIAAGGDGTLNEVVNGLVGHEHRPKFGIIPVGTTNDFARAIGVPRSIEEAADIICEGKT
EEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHCCCCHHHHHHHHEECCCC
VPLDLGRANDTYFINIAGGGRITELTYEVPSKLKTVLGQLAYYLKGIEMLPSLHPTYVEI
CEEECCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
EYDGKLLQEEITMFLITNTRSVGGFEKVAPYASINDGLFDLLVLKKGSIADLIKAATQAQ
EECCHHHHCCEEEEEEECCCCCCCHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHH
RGEHINNPKVLYTQANRIKVHSPDKLMINLDGEYGGDAPMEFENIYHCLELFVPEHQEDA
CCCCCCCCEEEEEECCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]