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The map label for this gene is guaA

Identifier: 49183286

GI number: 49183286

Start: 260912

End: 262459

Strand: Direct

Name: guaA

Synonym: BAS0254

Alternate gene names: 49183286

Gene position: 260912-262459 (Clockwise)

Preceding gene: 49183285

Following gene: 49183287

Centisome position: 4.99

GC content: 40.57

Gene sequence:

>1548_bases
GTGATTATTTTGAAGAAGCAACACGATACAATTATCGTTTTAGATTTTGGAAGTCAATACAATCAGTTAATAGCACGTCG
AATTCGTGAGTTCGGTGTATACAGTGAGCTTCATCCACATACAATTACTGCGGAAGAAATTAAAGCAATGAATCCAAAAG
GGATTATCTTCTCTGGTGGACCAAATAGTGTATACGGTGAAGGCGCATTACATTGTGATGAAAAAATCTTTGACCTAGGA
TTACCGATCTTCGGTATTTGTTACGGTATGCAACTTATGACGCAACAATTCGGTGGTACAGTAGAGCGTGCAAACCACCG
TGAGTACGGAAAAGCTGTTCTGAAAGTAGAGAACGAGTCAAAATTATATGCGAACCTTCCAGAAGAGCAAGTTGTATGGA
TGAGTCATGGCGACTTAGTAACTGGTTTACCTGAAGGATTCGTAGTAGACGCGACAAGTGAGTCTTGCCCAATTGCTGGT
ATGAGCAATGAAGCGAAAAACTTATACGGTGTACAATTCCACCCAGAAGTACGTCACTCTGAGCACGGTAACGATTTAAT
TAAAAACTTCGTATTCGGCGTATGTGGCTGTTCTGAAGGATGGAACATGGAGAACTTTATTGAAGTAGAACTAGAGAAAA
TCCGTGAAACTGTTGGAGACAAAAAAGTACTATGTGCGCTTAGCGGCGGTGTAGATTCTTCTGTTGTAGCAGTATTAATT
CATAAAGCAATCGGCGATCAGTTAACATGTATTTTCGTTGACCATGGTTTACTTCGTAAAGGCGAAGCAGAAGGTGTTAT
GAAAACATTTAGCGAAGGCTTCCACATGAACGTTATTAAAGTGGATGCAAAAGAACGCTTCATGAACAAGTTAAAAGGTG
TAGAAGATCCAGAACAAAAACGTAAAATTATCGGTAACGAATTCATTTACGTATTTGATGATGAAGCTTCTAAATTAGAA
GGAATGGACTTCTTAGCACAAGGTACACTGTACACAGATATCGTTGAAAGTGGTACAGCAACTGCACAAACAATTAAATC
TCACCATAACGTTGGTGGACTTCCAGAAGACATGCAGTTCAAATTAATTGAGCCTTTAAACACGTTATTTAAAGATGAAG
TACGTGTATTAGGATCTGAACTAGGAATTCCTGATGAGATCGTATGGCGTCAACCATTCCCAGGTCCTGGTCTTGGTATT
CGTGTACTAGGTGAAATCACGGAAGAGAAATTAGAAATCGTTCGTGAATCTGATGCGATTTTACGTGAAGAAATTATTAA
AGCAGGCTTAGACCGCGAAATCTGGCAATACTTCACTGCGCTTCCTGGTATGCGTAGCGTAGGTGTTATGGGTGACGAGC
GTACTTACGATTACACAGTGGGTATCCGTGCAGTAACATCTATCGACGGTATGACAGCTGACTGGGCACGTATCCCTTGG
GACGTATTAGAGAAAATCTCTGTACGTATCGTAAACGAAGTGAAACACGTTAACCGTATCGTGTATGATGTAACGAGTAA
GCCACCAGCAACTATTGAGTGGGAATAG

Upstream 100 bases:

>100_bases
TGTTATGAGACTAGAGGCGGAGGACTGGCTTTTTTTATTATATTGGTAATGCTTTTCGCCAAATTGGTGAAAATATTTAT
ATACGAGAACTAACGTTGGG

Downstream 100 bases:

>100_bases
TATTATAATAAAAGATCCATCTATTGCTCATGCAATAGATGGATCTTTTTTGATTTGAGTTTAATACGAACGAAATTATA
AAAGTTAATAAAAATGTTTG

Product: GMP synthase

Products: NA

Alternate protein names: GMP synthetase; Glutamine amidotransferase

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MIILKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGGPNSVYGEGALHCDEKIFDLG
LPIFGICYGMQLMTQQFGGTVERANHREYGKAVLKVENESKLYANLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAG
MSNEAKNLYGVQFHPEVRHSEHGNDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLI
HKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKVDAKERFMNKLKGVEDPEQKRKIIGNEFIYVFDDEASKLE
GMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGI
RVLGEITEEKLEIVRESDAILREEIIKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPW
DVLEKISVRIVNEVKHVNRIVYDVTSKPPATIEWE

Sequences:

>Translated_515_residues
MIILKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGGPNSVYGEGALHCDEKIFDLG
LPIFGICYGMQLMTQQFGGTVERANHREYGKAVLKVENESKLYANLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAG
MSNEAKNLYGVQFHPEVRHSEHGNDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLI
HKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKVDAKERFMNKLKGVEDPEQKRKIIGNEFIYVFDDEASKLE
GMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGI
RVLGEITEEKLEIVRESDAILREEIIKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPW
DVLEKISVRIVNEVKHVNRIVYDVTSKPPATIEWE
>Mature_515_residues
MIILKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGGPNSVYGEGALHCDEKIFDLG
LPIFGICYGMQLMTQQFGGTVERANHREYGKAVLKVENESKLYANLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAG
MSNEAKNLYGVQFHPEVRHSEHGNDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLI
HKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKVDAKERFMNKLKGVEDPEQKRKIIGNEFIYVFDDEASKLE
GMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGI
RVLGEITEEKLEIVRESDAILREEIIKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPW
DVLEKISVRIVNEVKHVNRIVYDVTSKPPATIEWE

Specific function: Catalyzes the synthesis of GMP from XMP

COG id: COG0519

COG function: function code F; GMP synthase, PP-ATPase domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GMP-binding domain

Homologues:

Organism=Homo sapiens, GI4504035, Length=546, Percent_Identity=37.1794871794872, Blast_Score=323, Evalue=3e-88,
Organism=Escherichia coli, GI1788854, Length=516, Percent_Identity=52.3255813953488, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789760, Length=154, Percent_Identity=35.7142857142857, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71992717, Length=566, Percent_Identity=36.3957597173145, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71992710, Length=566, Percent_Identity=36.3957597173145, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI133901714, Length=566, Percent_Identity=36.3957597173145, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6323873, Length=515, Percent_Identity=54.3689320388349, Blast_Score=554, Evalue=1e-158,
Organism=Saccharomyces cerevisiae, GI6322638, Length=154, Percent_Identity=32.4675324675325, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281365319, Length=552, Percent_Identity=35.5072463768116, Blast_Score=308, Evalue=5e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GUAA_BACAA (C3PBL1)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864893.1
- ProteinModelPortal:   C3PBL1
- SMR:   C3PBL1
- EnsemblBacteria:   EBBACT00000126146
- GeneID:   7850426
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0311
- GeneTree:   EBGT00050000001033
- ProtClustDB:   PRK00074
- HAMAP:   MF_00344
- InterPro:   IPR006220
- InterPro:   IPR001962
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR001674
- InterPro:   IPR004739
- InterPro:   IPR022955
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR00884
- TIGRFAMs:   TIGR00888

Pfam domain/function: PF00733 Asn_synthase; PF00117 GATase; PF00958 GMP_synt_C

EC number: =6.3.5.2

Molecular weight: Translated: 57580; Mature: 57580

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 87-87 ACT_SITE 174-174 ACT_SITE 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGG
CEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEECCC
PNSVYGEGALHCDEKIFDLGLPIFGICYGMQLMTQQFGGTVERANHREYGKAVLKVENES
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCEEEEECCCC
KLYANLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAGMSNEAKNLYGVQFHPEVRHS
CEEECCCCCEEEEEECCCEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC
EHGNDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLI
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
HKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKVDAKERFMNKLKGVEDPEQK
HHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCHHHH
RKIIGNEFIYVFDDEASKLEGMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMQF
HHHCCCCEEEEECCCCCHHCCCHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHH
KLIEPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGIRVLGEITEEKLEIVRESDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LREEIIKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPW
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHCCH
DVLEKISVRIVNEVKHVNRIVYDVTSKPPATIEWE
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECC
>Mature Secondary Structure
MIILKKQHDTIIVLDFGSQYNQLIARRIREFGVYSELHPHTITAEEIKAMNPKGIIFSGG
CEEEEECCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEECCC
PNSVYGEGALHCDEKIFDLGLPIFGICYGMQLMTQQFGGTVERANHREYGKAVLKVENES
CCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCHHHCCEEEEECCCC
KLYANLPEEQVVWMSHGDLVTGLPEGFVVDATSESCPIAGMSNEAKNLYGVQFHPEVRHS
CEEECCCCCEEEEEECCCEEECCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCC
EHGNDLIKNFVFGVCGCSEGWNMENFIEVELEKIRETVGDKKVLCALSGGVDSSVVAVLI
CCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHH
HKAIGDQLTCIFVDHGLLRKGEAEGVMKTFSEGFHMNVIKVDAKERFMNKLKGVEDPEQK
HHHHCCCEEEEEECCCHHCCCCHHHHHHHHCCCCEEEEEEECHHHHHHHHHCCCCCHHHH
RKIIGNEFIYVFDDEASKLEGMDFLAQGTLYTDIVESGTATAQTIKSHHNVGGLPEDMQF
HHHCCCCEEEEECCCCCHHCCCHHHHCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHH
KLIEPLNTLFKDEVRVLGSELGIPDEIVWRQPFPGPGLGIRVLGEITEEKLEIVRESDAI
HHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LREEIIKAGLDREIWQYFTALPGMRSVGVMGDERTYDYTVGIRAVTSIDGMTADWARIPW
HHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCHHHCCH
DVLEKISVRIVNEVKHVNRIVYDVTSKPPATIEWE
HHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA