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The map label for this gene is ddl [H]

Identifier: 49183263

GI number: 49183263

Start: 232006

End: 232482

Strand: Direct

Name: ddl [H]

Synonym: BAS0230

Alternate gene names: 49183263

Gene position: 232006-232482 (Clockwise)

Preceding gene: 49183262

Following gene: 49183264

Centisome position: 4.44

GC content: 35.64

Gene sequence:

>477_bases
GTGACAAAAATTAAATTAGGTTTATTATATGGTGGAAAATCAGCTGAGCATCAAGTTTCGTTACAAACGGCTCTTGCTGC
TATTAAAGCATTAAATCAAGATAAATTCGAGATTCATCCAATTTATATTACAGAACAAGGTCAATGGATACGCGGTGAAC
GTATTGAAGGTGAAGTAGCAAATGTTGAAGTTCTAAAAATGAGCGGTGCAGAAAATGCGATTTCTCCGTTATCTTTAAGC
ACAGAAATCATTCCATCTGCAAATGCTGAGGAAAATGCTATCGACGTTATTTTCCCATTACTACATGGACCAAATGGTGA
AGATGGAACGGTTCAAGGTTTATTAGAATTAATGAACATTCCTTATGTAGGAAATGGTGTTCTAGCATCTGCTGCTGGTA
TGGATAAAGTTGTTATGAAAAACATTTTCGCAGAAGCTGGATTAAAAACAAGCGAAATATGCATCATTCATTCGTAG

Upstream 100 bases:

>100_bases
ATTATTTGTGGAAGTTACATTTTATTTCAAATAGGAACTACCTATTTTTATAAAAAGTGCTAAAATAGAAAAATGAAAAC
AATTGGTTAGGGTGATTTCA

Downstream 100 bases:

>100_bases
CGGATGGGAAAAAGATCGTGAAACAGCATATGAAAAAGTAGAAGAAGTATTAGGATACCCTTGCTTCGTAAAACCAGCAA
ACCTTGGTTCAAGTGTTGGG

Product: D-alanine-D-alanine ligase, N-terminus

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKTSEICIIHS

Sequences:

>Translated_158_residues
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLS
TEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKTSEICIIHS
>Mature_157_residues
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVANVEVLKMSGAENAISPLSLST
EIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNIPYVGNGVLASAAGMDKVVMKNIFAEAGLKTSEICIIHS

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=154, Percent_Identity=39.6103896103896, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1786280, Length=144, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 16881; Mature: 16749

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00843 DALA_DALA_LIGASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVA
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCEEC
NVEVLKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKTSEICIIHS
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
TKIKLGLLYGGKSAEHQVSLQTALAAIKALNQDKFEIHPIYITEQGQWIRGERIEGEVA
CEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCEECCCCCCCEEC
NVEVLKMSGAENAISPLSLSTEIIPSANAEENAIDVIFPLLHGPNGEDGTVQGLLELMNI
CEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCC
PYVGNGVLASAAGMDKVVMKNIFAEAGLKTSEICIIHS
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA