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The map label for this gene is tuf [H]

Identifier: 49183142

GI number: 49183142

Start: 119377

End: 120564

Strand: Direct

Name: tuf [H]

Synonym: BAS0108

Alternate gene names: 49183142

Gene position: 119377-120564 (Clockwise)

Preceding gene: 49183141

Following gene: 49183143

Centisome position: 2.28

GC content: 43.94

Gene sequence:

>1188_bases
ATGGCTAAAGCTAAATTCGAACGTTCTAAACCCCATGTTAACATCGGTACAATCGGCCACGTTGACCATGGTAAAACTAC
ATTAACTGCTGCGATCACTACAGTACTTGCAAAAGCTGGTGGTGCTGAAGCACGCGGATACGATCAAATCGATGCTGCTC
CAGAAGAAAGAGAGCGCGGTATCACAATCTCAACTGCACACGTTGAGTACGAAACTGAAACTCGTCACTATGCACACGTT
GACTGCCCAGGTCACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCTCAAATGGACGGCGGTATCTTAGTAGTATC
TGCTGCTGATGGCCCAATGCCTCAAACTCGTGAGCACATCCTTCTTTCTCGTCAAGTAGGTGTACCTTACATCGTTGTAT
TCTTAAACAAATGCGACATGGTAGACGACGAAGAATTATTAGAATTAGTAGAAATGGAAGTTCGCGACCTATTATCTGAA
TACGGATTCCCAGGCGACGACATTCCTGTAATCAAAGGTTCTGCTCTTAAAGCTCTTCAAGGAGAAGCTGATTGGGAAGC
AAAAATCATTGAATTAATGGCTGAAGTTGATGCTTACATCCCAACTCCAGAACGTGAAACTGACAAACCATTCTTAATGC
CTGTAGAGGACGTATTCTCTATCACAGGTCGTGGTACAGTTGCTACTGGTCGTGTTGAGCGCGGTATCGTTAAAGTTGGT
GACGTAGTAGAAATCATCGGTCTTGCTGAAGAAAATGCTTCTACAACTGTAACTGGTGTAGAGATGTTCCGTAAACTTCT
TGACCAAGCTCAAGCTGGAGACAACATCGGTGCTTTACTTCGTGGGGTTGCTCGTGAAGACATCCAACGTGGACAAGTAC
TTGCAAAAAGCGGTTCTGTAAAAGCTCACGCTAAATTCAAAGCTGAAGTTTTCGTATTATCTAAAGAAGAAGGTGGACGT
CACACTCCATTCTTCGCTAACTACCGTCCTCAGTTCTACTTCCGTACAACTGACGTAACTGGTATCATCCAATTACCAGA
AGGTACTGAAATGGTAATGCCTGGTGACAACATCGAAATGACTATCGAACTTATCGCTCCAATCGCTATCGAAGAGGGAA
CTAAATTCTCTATTCGTGAAGGTGGACGTACAGTAGGTTACGGTGTAGTTGCTACTATCGTTGAGTAA

Upstream 100 bases:

>100_bases
TTCACGTATAACTACTTATGTAAGCTTAGAAAGTGGGACGCAAGTTTCGCTTTCTAGCCTAAATATAAAATAACCTATAT
AAACTAAGGAGGAATTTAGA

Downstream 100 bases:

>100_bases
TCTTAACTGATATAAAAAACCCCGAGGGATTTTCCCTTGGGGTTTTTGTTTGCGTTTATATAATAAATAAAATCATATTT
TTATGTTTTGACGAGTTTCT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YGFPGDDIPVIKGSALKALQGEADWEAKIIELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGIVKVG
DVVEIIGLAEENASTTVTGVEMFRKLLDQAQAGDNIGALLRGVAREDIQRGQVLAKSGSVKAHAKFKAEVFVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNIEMTIELIAPIAIEEGTKFSIREGGRTVGYGVVATIVE

Sequences:

>Translated_395_residues
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHV
DCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YGFPGDDIPVIKGSALKALQGEADWEAKIIELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGIVKVG
DVVEIIGLAEENASTTVTGVEMFRKLLDQAQAGDNIGALLRGVAREDIQRGQVLAKSGSVKAHAKFKAEVFVLSKEEGGR
HTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNIEMTIELIAPIAIEEGTKFSIREGGRTVGYGVVATIVE
>Mature_394_residues
AKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERGITISTAHVEYETETRHYAHVD
CPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEY
GFPGDDIPVIKGSALKALQGEADWEAKIIELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGIVKVGD
VVEIIGLAEENASTTVTGVEMFRKLLDQAQAGDNIGALLRGVAREDIQRGQVLAKSGSVKAHAKFKAEVFVLSKEEGGRH
TPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNIEMTIELIAPIAIEEGTKFSIREGGRTVGYGVVATIVE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=56.8181818181818, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI4503475, Length=357, Percent_Identity=33.3333333333333, Blast_Score=175, Evalue=8e-44,
Organism=Homo sapiens, GI4503471, Length=450, Percent_Identity=29.3333333333333, Blast_Score=172, Evalue=6e-43,
Organism=Homo sapiens, GI223555963, Length=446, Percent_Identity=29.8206278026906, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=446, Percent_Identity=29.8206278026906, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI194018522, Length=345, Percent_Identity=27.8260869565217, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194097354, Length=344, Percent_Identity=27.906976744186, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI54607086, Length=321, Percent_Identity=28.6604361370716, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI194018520, Length=344, Percent_Identity=27.906976744186, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI46094014, Length=331, Percent_Identity=27.7945619335347, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI4503507, Length=358, Percent_Identity=27.6536312849162, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI157426893, Length=236, Percent_Identity=29.6610169491525, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=75.6345177664975, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=75.6345177664975, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI2367247, Length=370, Percent_Identity=30, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI48994988, Length=240, Percent_Identity=32.9166666666667, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1789108, Length=321, Percent_Identity=31.7757009345794, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=31.5068493150685, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=52.1303258145363, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=386, Percent_Identity=42.7461139896373, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=452, Percent_Identity=30.9734513274336, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17569207, Length=452, Percent_Identity=30.9734513274336, Blast_Score=181, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=29.7235023041475, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32566629, Length=458, Percent_Identity=27.292576419214, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI115532067, Length=429, Percent_Identity=27.2727272727273, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=428, Percent_Identity=27.3364485981308, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17506081, Length=266, Percent_Identity=29.6992481203008, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=150, Percent_Identity=37.3333333333333, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=288, Percent_Identity=26.7361111111111, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=28.8793103448276, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17565438, Length=426, Percent_Identity=23.7089201877934, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=398, Percent_Identity=65.3266331658292, Blast_Score=532, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=30.1801801801802, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=30.1801801801802, Blast_Score=176, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6322937, Length=349, Percent_Identity=26.0744985673352, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=352, Percent_Identity=28.4090909090909, Blast_Score=93, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6320863, Length=267, Percent_Identity=28.0898876404494, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=238, Percent_Identity=29.8319327731092, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=34.8148148148148, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=58.6294416243655, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=58.6294416243655, Blast_Score=482, Evalue=1e-136,
Organism=Drosophila melanogaster, GI19921738, Length=385, Percent_Identity=48.3116883116883, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI45553807, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45553816, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24651721, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17864154, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI24652838, Length=450, Percent_Identity=30, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17137572, Length=450, Percent_Identity=30, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.6732673267327, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17137380, Length=342, Percent_Identity=29.5321637426901, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550900, Length=338, Percent_Identity=29.585798816568, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24647046, Length=290, Percent_Identity=26.2068965517241, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647048, Length=335, Percent_Identity=26.2686567164179, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=242, Percent_Identity=32.2314049586777, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582462, Length=165, Percent_Identity=32.7272727272727, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI78706572, Length=236, Percent_Identity=27.9661016949153, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42939; Mature: 42808

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHCCCCCEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYGFPGDDIPVIKGSALKALQ
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEADWEAKIIELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGIVKVG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECHHHCCEEECC
DVVEIIGLAEENASTTVTGVEMFRKLLDQAQAGDNIGALLRGVAREDIQRGQVLAKSGSV
CHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCE
KAHAKFKAEVFVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNIEM
EEEEEEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TIELIAPIAIEEGTKFSIREGGRTVGYGVVATIVE
EEEEEEEEEECCCCEEEECCCCCEECCEEEEEEEC
>Mature Secondary Structure 
AKAKFERSKPHVNIGTIGHVDHGKTTLTAAITTVLAKAGGAEARGYDQIDAAPEERERG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCC
ITISTAHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHCCCCCEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYGFPGDDIPVIKGSALKALQ
EEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEADWEAKIIELMAEVDAYIPTPERETDKPFLMPVEDVFSITGRGTVATGRVERGIVKVG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHCCCCEEEECHHHCCEEECC
DVVEIIGLAEENASTTVTGVEMFRKLLDQAQAGDNIGALLRGVAREDIQRGQVLAKSGSV
CHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCEEECCCCE
KAHAKFKAEVFVLSKEEGGRHTPFFANYRPQFYFRTTDVTGIIQLPEGTEMVMPGDNIEM
EEEEEEEEEEEEEECCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCEEEECCCCCEE
TIELIAPIAIEEGTKFSIREGGRTVGYGVVATIVE
EEEEEEEEEECCCCEEEECCCCCEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA