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The map label for this gene is ksgA

Identifier: 49183075

GI number: 49183075

Start: 45283

End: 46161

Strand: Direct

Name: ksgA

Synonym: BAS0040

Alternate gene names: 49183075

Gene position: 45283-46161 (Clockwise)

Preceding gene: 49183074

Following gene: 49183076

Centisome position: 0.87

GC content: 36.06

Gene sequence:

>879_bases
ATGAAGGATATCGCAACGCCAAATCGTACGAAAGACATTGTTGAAAAGTATGGATTTTCATTCAAAAAAAGTTTAGGACA
AAATTTTTTAATTGATACGAATGTATTAAATCGTATCGTTGATCACGCTGAAATTGGTTCAGAAAGTGGTGCAATTGAAA
TTGGACCAGGTATCGGTGCATTAACAGAACAATTAGCGAAGCGTGCCAAAAAAGTAGTGGCTTTTGAAATTGATCAAAGG
CTATTACCAATTTTAGATGAGACGTTAGCTCCATATGGAAACGTTACAGTTATCAATAAAGATGTACTAAAGGCAGATGT
ACATGAGGTGTTTAGTGAGCAATTTGAAGAAGGACAAGATGTAATGGTAGTAGCTAACTTACCATACTATATTACAACGC
CAATTTTATTTAAATTGCTGGAAGAGAAATTACCGGTTCGTGGATTCGTTGTTATGATGCAAAAAGAAGTTGGGGATCGT
TTAGCGGCTAAGCCTGGAACGAAAGAGTACGGTTCTTTATCCATTGCTATTCAGTATTATACAGAGGTAGAAACAGTTAT
GACTGTACCGCGTACAGTGTTTGTGCCACAACCAAATGTTGATTCTGCAATTATTCGCCTCCTAAAGCGTCCGAAACCAG
TAGTAGAAGTAACAGATGAGACATTCTTCTTTGAAGTAGTACGAGCAAGTTTTGCACAACGCCGTAAAACTTTAATGAAT
AATTTATCAAATAATTTAAATGGTTTCCCGAAAGATAAAGAGCTGTTGGATCGAATTTTAACAGAAGTAGGAATTGATCC
AAAACGAAGAGGCGAAACGCTATCTATCGAAGAGTTTGCGACATTAAGTAATGCATTAGTTCTTCATAAGTTATCATAA

Upstream 100 bases:

>100_bases
ATACAAACGCAAAACAGTTACATAAACGTTTACAAATGTTTCAAGTCTCAAAAGAATCATTTGCAGAAGCTTATAAGCAA
GTCATACAGGAGGAAAGAAA

Downstream 100 bases:

>100_bases
GAATACAAAAGGGACAGTTCAATTTGAACTGTCCCTTTTGTCACCTTTCTCCTCCTAAATTCATACTTTAAAAACAGGTA
AGATGGCCTAACGAGTTTGG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MKDIATPNRTKDIVEKYGFSFKKSLGQNFLIDTNVLNRIVDHAEIGSESGAIEIGPGIGALTEQLAKRAKKVVAFEIDQR
LLPILDETLAPYGNVTVINKDVLKADVHEVFSEQFEEGQDVMVVANLPYYITTPILFKLLEEKLPVRGFVVMMQKEVGDR
LAAKPGTKEYGSLSIAIQYYTEVETVMTVPRTVFVPQPNVDSAIIRLLKRPKPVVEVTDETFFFEVVRASFAQRRKTLMN
NLSNNLNGFPKDKELLDRILTEVGIDPKRRGETLSIEEFATLSNALVLHKLS

Sequences:

>Translated_292_residues
MKDIATPNRTKDIVEKYGFSFKKSLGQNFLIDTNVLNRIVDHAEIGSESGAIEIGPGIGALTEQLAKRAKKVVAFEIDQR
LLPILDETLAPYGNVTVINKDVLKADVHEVFSEQFEEGQDVMVVANLPYYITTPILFKLLEEKLPVRGFVVMMQKEVGDR
LAAKPGTKEYGSLSIAIQYYTEVETVMTVPRTVFVPQPNVDSAIIRLLKRPKPVVEVTDETFFFEVVRASFAQRRKTLMN
NLSNNLNGFPKDKELLDRILTEVGIDPKRRGETLSIEEFATLSNALVLHKLS
>Mature_292_residues
MKDIATPNRTKDIVEKYGFSFKKSLGQNFLIDTNVLNRIVDHAEIGSESGAIEIGPGIGALTEQLAKRAKKVVAFEIDQR
LLPILDETLAPYGNVTVINKDVLKADVHEVFSEQFEEGQDVMVVANLPYYITTPILFKLLEEKLPVRGFVVMMQKEVGDR
LAAKPGTKEYGSLSIAIQYYTEVETVMTVPRTVFVPQPNVDSAIIRLLKRPKPVVEVTDETFFFEVVRASFAQRRKTLMN
NLSNNLNGFPKDKELLDRILTEVGIDPKRRGETLSIEEFATLSNALVLHKLS

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=289, Percent_Identity=32.1799307958478, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI156415992, Length=294, Percent_Identity=30.6122448979592, Blast_Score=118, Evalue=5e-27,
Organism=Escherichia coli, GI1786236, Length=271, Percent_Identity=34.6863468634686, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI25146882, Length=285, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=28.8135593220339, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324989, Length=281, Percent_Identity=29.5373665480427, Blast_Score=116, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21358017, Length=235, Percent_Identity=35.3191489361702, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI21357273, Length=298, Percent_Identity=27.1812080536913, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_BACAA (C3P9I6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002864692.1
- ProteinModelPortal:   C3P9I6
- SMR:   C3P9I6
- EnsemblBacteria:   EBBACT00000127566
- GeneID:   7848457
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_0050
- GeneTree:   EBGT00050000002781
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 32768; Mature: 32768

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 76-76 BINDING 101-101 BINDING 126-126

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDIATPNRTKDIVEKYGFSFKKSLGQNFLIDTNVLNRIVDHAEIGSESGAIEIGPGIGA
CCCCCCCCHHHHHHHHHCCHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCHH
LTEQLAKRAKKVVAFEIDQRLLPILDETLAPYGNVTVINKDVLKADVHEVFSEQFEEGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
VMVVANLPYYITTPILFKLLEEKLPVRGFVVMMQKEVGDRLAAKPGTKEYGSLSIAIQYY
EEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEH
TEVETVMTVPRTVFVPQPNVDSAIIRLLKRPKPVVEVTDETFFFEVVRASFAQRRKTLMN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
NLSNNLNGFPKDKELLDRILTEVGIDPKRRGETLSIEEFATLSNALVLHKLS
HHHHCCCCCCCCHHHHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKDIATPNRTKDIVEKYGFSFKKSLGQNFLIDTNVLNRIVDHAEIGSESGAIEIGPGIGA
CCCCCCCCHHHHHHHHHCCHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCHH
LTEQLAKRAKKVVAFEIDQRLLPILDETLAPYGNVTVINKDVLKADVHEVFSEQFEEGQD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
VMVVANLPYYITTPILFKLLEEKLPVRGFVVMMQKEVGDRLAAKPGTKEYGSLSIAIQYY
EEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEH
TEVETVMTVPRTVFVPQPNVDSAIIRLLKRPKPVVEVTDETFFFEVVRASFAQRRKTLMN
HHHHHHHHCCCEEECCCCCCCHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHHH
NLSNNLNGFPKDKELLDRILTEVGIDPKRRGETLSIEEFATLSNALVLHKLS
HHHHCCCCCCCCHHHHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA