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The map label for this gene is dacA [H]

Identifier: 49183048

GI number: 49183048

Start: 17037

End: 18353

Strand: Direct

Name: dacA [H]

Synonym: BAS0012

Alternate gene names: 49183048

Gene position: 17037-18353 (Clockwise)

Preceding gene: 49183047

Following gene: 49183049

Centisome position: 0.33

GC content: 35.23

Gene sequence:

>1317_bases
GTGAAAGGTATGTTTTGCAAAAGATTCATTGCACTAGTAACGATGCTTACACTAGTTTGTAGTATGTTGTTACCATATAG
CAATGCGTCAGCAGAAACAGGATCTGCTTTAAACATTGAAGCAGGTGCGGCAATTTTAGTTGAAGCGAATTCTGGAAAAA
TTTTATATCAAAAGAATGCAGATGAATTATTATCAATTGCTAGTATGACAAAGATGATGAGTGAATATTTAGTTCACGAA
GCAGTGGATAAAGGGAAGCTTAAGTGGGATCAGAAAATTAAGGTTTCTGAATATGCGTATAAAGTTTCACAAGATGCTTC
ATTATCAAATGTGGCATTAGAAAATGGTGGTTCTTATACGGTAAAAGAGTTGTACGAGGCAATGGCAATCTTCTCTGCAA
ACGGTGCAACGATTGCATTAGCAGAAGCAATTGCAGGTAAAGAAGTAGATTTCGTAAAAATGATGAATGATAAATCGAAA
GAGCTAGGGTTGAAAAATTATAAATTTGTCAATTCTACAGGTTTAACGAATAAGGATTTAAAGGGAATGCATCCAGAAGG
AACAACAGCAGATGAAGAAAATAAAATGTCTGCAAAGGATGTTGCAACTTTAGCACAACATTTAATTAAAGATTATCCGA
AAGTGTTAGATACAGCAAAAATCCCGAAAAAAGAATTCCGTCCAGAAAAAGAGAAGTTTGCAATGTCGAACTGGAACTGG
ATGTTAAAAGGTTTAGTTAAAGAATATGATGGCGTAGATGGCCTAAAAACAGGTTCAACTCCAGAAGCAGGAGATTGCTT
CACTGGTACGGTTGAAAGAAACGGTATGCGTTTTATTTCTGTAGTAATTAAAACAAGTTCTCATACAGCACGTTTTGATG
AAACAAAGAAGCTATATGATTATGGATTTGCTAACTTTGAAATGAAACAAATGTATAAAAAAGGTTCTTCAGTAAAAGGA
CAAGAAACAGTACGAGTAGAAAATGCGAAAGATAAGGATGTAGCAGTTCAAACGAAACAAGCCATTTCACTTCCAGTACC
AAAAGGAAGTAAAGAAGTTTATAAAACAGAATTAAAAGAATCAAGTAAAGGACAAGAAGCACCTATTAAAAAAGGAGCTG
CGCTTGGTCAAATGGTAATTACGCCAAAAGATACAAACGACCCTGGATTTTTAACTGGTAAGTCGTTGCAAGTGGATCTT
GTAACAACATCTGAAGTAGAAGAAGCAAACTGGTTTACTCGTGCTATGCGCGGAATTGGTTCTTTCTTTAGTGGTATATG
GAATAGTGCTGTTGATACAGTAAAAGGTTGGTTTTAA

Upstream 100 bases:

>100_bases
TTATATACAAATAGACGGAGATTTGATATCTCTGTCTATTTTTTTTTGATTATGTTAGAATAACGGTTATGTGAGTACAT
AGATATTGGGGGTAGCAAAA

Downstream 100 bases:

>100_bases
AAGCTCCTCATTGTAGGGGCTTTTTCTTATTCCTATTTTTCATACCGACTTTATGAAAAAGTAGTAGACAAGCATCTGAT
AGTTAGTGGTAGAATGTAAG

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHE
AVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSK
ELGLKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNW
MLKGLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKG
QETVRVENAKDKDVAVQTKQAISLPVPKGSKEVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDL
VTTSEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF

Sequences:

>Translated_438_residues
MKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHE
AVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSK
ELGLKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNW
MLKGLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKG
QETVRVENAKDKDVAVQTKQAISLPVPKGSKEVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDL
VTTSEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF
>Mature_438_residues
MKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNADELLSIASMTKMMSEYLVHE
AVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYTVKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSK
ELGLKNYKFVNSTGLTNKDLKGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNW
MLKGLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYDYGFANFEMKQMYKKGSSVKG
QETVRVENAKDKDVAVQTKQAISLPVPKGSKEVYKTELKESSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDL
VTTSEVEEANWFTRAMRGIGSFFSGIWNSAVDTVKGWF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=420, Percent_Identity=30.4761904761905, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI1786851, Length=441, Percent_Identity=28.3446712018141, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87082022, Length=400, Percent_Identity=27.75, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI87082054, Length=266, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 48215; Mature: 48215

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEECCH
DELLSIASMTKMMSEYLVHEAVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCHHHEEECCCCCEE
VKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELGLKNYKFVNSTGLTNKDL
HHHHHHHHHHHCCCCCEEEEHHHHCCCCCHHHHHHCCCHHHCCCCCEEEECCCCCCCCCC
KGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCHHH
MLKGLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYD
HHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
YGFANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAISLPVPKGSKEVYKTELKE
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHEECCCCCCCHHHHHHHHHH
SSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTTSEVEEANWFTRAMRGIG
CCCCCCCCHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHH
SFFSGIWNSAVDTVKGWF
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGMFCKRFIALVTMLTLVCSMLLPYSNASAETGSALNIEAGAAILVEANSGKILYQKNA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCEEEEEECCCCEEEEECCH
DELLSIASMTKMMSEYLVHEAVDKGKLKWDQKIKVSEYAYKVSQDASLSNVALENGGSYT
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCCCHHHEEECCCCCEE
VKELYEAMAIFSANGATIALAEAIAGKEVDFVKMMNDKSKELGLKNYKFVNSTGLTNKDL
HHHHHHHHHHHCCCCCEEEEHHHHCCCCCHHHHHHCCCHHHCCCCCEEEECCCCCCCCCC
KGMHPEGTTADEENKMSAKDVATLAQHLIKDYPKVLDTAKIPKKEFRPEKEKFAMSNWNW
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHCCHHH
MLKGLVKEYDGVDGLKTGSTPEAGDCFTGTVERNGMRFISVVIKTSSHTARFDETKKLYD
HHHHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
YGFANFEMKQMYKKGSSVKGQETVRVENAKDKDVAVQTKQAISLPVPKGSKEVYKTELKE
CCCCCHHHHHHHHCCCCCCCCCEEEECCCCCCCCEEEHHHHEECCCCCCCHHHHHHHHHH
SSKGQEAPIKKGAALGQMVITPKDTNDPGFLTGKSLQVDLVTTSEVEEANWFTRAMRGIG
CCCCCCCCHHHCCCCCCEEECCCCCCCCCEEECCCEEEEEEECCCHHHHHHHHHHHHHHH
SFFSGIWNSAVDTVKGWF
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 6768745; 3087956; 6780559; 111240 [H]