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The map label for this gene is pyk [H]

Identifier: 46447270

GI number: 46447270

Start: 1956411

End: 1958207

Strand: Reverse

Name: pyk [H]

Synonym: pc1636

Alternate gene names: 46447270

Gene position: 1958207-1956411 (Counterclockwise)

Preceding gene: 46447272

Following gene: 46447269

Centisome position: 81.1

GC content: 37.28

Gene sequence:

>1797_bases
ATGGCATTAATTAGAACAAAAATTGTTTGTACAATAGGACCTGCTTGCAATTCCTTAGAGAAAATTATTGAGTTAATAAA
CGTAGGAATGAACGTTGCAAGGTTGAATTTTAGTCATGGAACTCAAGAAGAACATTTAAGGACAATTAATCTTTTAAAAG
AAGCGAGATGTCAACTTAACTTACCTTTAGCCATCATGTTAGATACAAAAGGGCCAGAAATACGGCTAGGAAAAATTCGA
GATGGACAAATTTTTTTAACGGTAGGTCAAAAATGGCGGTTAGTGAAAAAGGAGGTTTTAGGAGATGAAAGTCAGGTTTC
TATTTTTCCTCTCAATATTTTAGATCAACTTCCTGTTGGGACAACAATTTTATTTGATGATGGGTATATTGCCTCACGCG
TGATTGAAAATTCATCCGAAGGTGTGCTTGTTGAAATTAATAACAGCGGGATGATTCGTTCAAGTAAAGGAGTTAATATT
CCCAATACAAGCCTAAATTTGCCTGCTGTCACCGAAAAAGACATTGACGATATTCGTTTTGGATGTAGCCAGGATATTGA
CTTGATTGCTGCTTCCTTTGTTCGTTCTGCAGAACATGTCTTGGAGATTAAGCGTTTATTAGCTGATGAAAAAAAAACTG
ATATTCTAGTTATTGCAAAAATTGAAAATAGTGAAGGGGTGCAAAATTTTGATAGCATTGTCCAAGCAGCAGACGGAATC
ATGATAGCAAGAGGAGATTTAGGGGTTGAAGTTCCTTTAAGCCATGTTCCTAGATTGCAGAAAATGATGATCCGTAAAAG
TTATTTGGCAGGCAAGCCAGTTGTGACAGCAACGCAGATGTTAGAATCAATGATCAATAATCCTAGACCAACTCGTGCAG
AAACATCTGATGTAGCAAATGCGATTTATGATAGTACATCTGCTATCATGCTTTCAGGAGAAACAGCAATTGGTCGTTAT
CCTGTTGAGACGGTTAATGTGATGCGAAGTATCGTAGAAGAGGCTGAAGCTGATTTCAACTACAGTACTTTTTTTGATCA
ACATGCCCCTTTAGTTTATCATGATGTTCCCTCTGCTGTGACTTTAGCTACTGTAAAAACAGCTTACAGTTCTAGCGCTA
AAGCTATTTTTGCTTTTACTAAAGCAGGAACAACGGCACGACTTTTATCAAGACTTAGGCCTAAAATGCCTATTATTGCG
ATGACAGCAAAAGAAAAAATTTTTCATCAGTTGGCATTTAATTGGGGAGTTATTCCTTTTTTAAGTGAACCTTGTAACTC
TTTAGATAAAAGTTTAAAAAAAATCAGTGATTTCGCCCTTGGAAGTCAACACGTCAGCTATGGAGATTTGGTTGTGATTA
CTGCAGGAACTCCTTTTGGATTTTCAGGAACGACCAATATGATGATCGTTGAAAATATTGGAGATGTTTTGGTAAGAGGG
CACTCTGGATATGGTATACGTGTGCATGGGAACGTAGTAATAGTTCTTGCTCCTCAGTCTAAAGCTCCTTATGCGGTCAA
AGATCAGGTACTTGTGATTGCCAAATGCGATGAGAGTTATTTATCTTTAATGAAGGAAGCTTCTGCTATTATTTTACAGA
ACCATATCAATGATTTTGCGTCTGAAAAATACGCGAAGAAGATTGCTCTGAGTTTAGGTAAGCCTATTCTTCTTCGAGCT
GATGCGGCATGTCAAATTTTAAAAGAAGGACAGCTTGTTACTCTCGATCCTGAAAAAGCCCTTGTTTACAAAGGAGTTGT
TTTGGACCAATTGTTGGCGAGTGGAGAATTTTCATGA

Upstream 100 bases:

>100_bases
GACTTTTAAAGTTTTAGCAATAATTAAGTTACATTTGTCAAAATAGTCCCTAGTGATTAAATTAAATCAAAGCACGTTCA
AATAGAAACTCAGGAAAAAT

Downstream 100 bases:

>100_bases
GTGCTGTCCAAGCATTCCCTTTTCATCTATTTTCTTATATTTCGCCATTTTACTATCAAAATTATGTTGTAAACAGCAAC
CAACAAACTTTTGTTTTTCA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MALIRTKIVCTIGPACNSLEKIIELINVGMNVARLNFSHGTQEEHLRTINLLKEARCQLNLPLAIMLDTKGPEIRLGKIR
DGQIFLTVGQKWRLVKKEVLGDESQVSIFPLNILDQLPVGTTILFDDGYIASRVIENSSEGVLVEINNSGMIRSSKGVNI
PNTSLNLPAVTEKDIDDIRFGCSQDIDLIAASFVRSAEHVLEIKRLLADEKKTDILVIAKIENSEGVQNFDSIVQAADGI
MIARGDLGVEVPLSHVPRLQKMMIRKSYLAGKPVVTATQMLESMINNPRPTRAETSDVANAIYDSTSAIMLSGETAIGRY
PVETVNVMRSIVEEAEADFNYSTFFDQHAPLVYHDVPSAVTLATVKTAYSSSAKAIFAFTKAGTTARLLSRLRPKMPIIA
MTAKEKIFHQLAFNWGVIPFLSEPCNSLDKSLKKISDFALGSQHVSYGDLVVITAGTPFGFSGTTNMMIVENIGDVLVRG
HSGYGIRVHGNVVIVLAPQSKAPYAVKDQVLVIAKCDESYLSLMKEASAIILQNHINDFASEKYAKKIALSLGKPILLRA
DAACQILKEGQLVTLDPEKALVYKGVVLDQLLASGEFS

Sequences:

>Translated_598_residues
MALIRTKIVCTIGPACNSLEKIIELINVGMNVARLNFSHGTQEEHLRTINLLKEARCQLNLPLAIMLDTKGPEIRLGKIR
DGQIFLTVGQKWRLVKKEVLGDESQVSIFPLNILDQLPVGTTILFDDGYIASRVIENSSEGVLVEINNSGMIRSSKGVNI
PNTSLNLPAVTEKDIDDIRFGCSQDIDLIAASFVRSAEHVLEIKRLLADEKKTDILVIAKIENSEGVQNFDSIVQAADGI
MIARGDLGVEVPLSHVPRLQKMMIRKSYLAGKPVVTATQMLESMINNPRPTRAETSDVANAIYDSTSAIMLSGETAIGRY
PVETVNVMRSIVEEAEADFNYSTFFDQHAPLVYHDVPSAVTLATVKTAYSSSAKAIFAFTKAGTTARLLSRLRPKMPIIA
MTAKEKIFHQLAFNWGVIPFLSEPCNSLDKSLKKISDFALGSQHVSYGDLVVITAGTPFGFSGTTNMMIVENIGDVLVRG
HSGYGIRVHGNVVIVLAPQSKAPYAVKDQVLVIAKCDESYLSLMKEASAIILQNHINDFASEKYAKKIALSLGKPILLRA
DAACQILKEGQLVTLDPEKALVYKGVVLDQLLASGEFS
>Mature_597_residues
ALIRTKIVCTIGPACNSLEKIIELINVGMNVARLNFSHGTQEEHLRTINLLKEARCQLNLPLAIMLDTKGPEIRLGKIRD
GQIFLTVGQKWRLVKKEVLGDESQVSIFPLNILDQLPVGTTILFDDGYIASRVIENSSEGVLVEINNSGMIRSSKGVNIP
NTSLNLPAVTEKDIDDIRFGCSQDIDLIAASFVRSAEHVLEIKRLLADEKKTDILVIAKIENSEGVQNFDSIVQAADGIM
IARGDLGVEVPLSHVPRLQKMMIRKSYLAGKPVVTATQMLESMINNPRPTRAETSDVANAIYDSTSAIMLSGETAIGRYP
VETVNVMRSIVEEAEADFNYSTFFDQHAPLVYHDVPSAVTLATVKTAYSSSAKAIFAFTKAGTTARLLSRLRPKMPIIAM
TAKEKIFHQLAFNWGVIPFLSEPCNSLDKSLKKISDFALGSQHVSYGDLVVITAGTPFGFSGTTNMMIVENIGDVLVRGH
SGYGIRVHGNVVIVLAPQSKAPYAVKDQVLVIAKCDESYLSLMKEASAIILQNHINDFASEKYAKKIALSLGKPILLRAD
AACQILKEGQLVTLDPEKALVYKGVVLDQLLASGEFS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=485, Percent_Identity=41.8556701030928, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI33286420, Length=485, Percent_Identity=41.8556701030928, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI10835121, Length=494, Percent_Identity=42.1052631578947, Blast_Score=355, Evalue=7e-98,
Organism=Homo sapiens, GI33286418, Length=485, Percent_Identity=41.8556701030928, Blast_Score=355, Evalue=7e-98,
Organism=Homo sapiens, GI32967597, Length=494, Percent_Identity=42.1052631578947, Blast_Score=355, Evalue=9e-98,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=45.1048951048951, Blast_Score=236, Evalue=6e-62,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=45.1048951048951, Blast_Score=236, Evalue=6e-62,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=42.5531914893617, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=42.5531914893617, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=41.2037037037037, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=43.7768240343348, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788160, Length=485, Percent_Identity=36.0824742268041, Blast_Score=271, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=42.8571428571429, Blast_Score=357, Evalue=7e-99,
Organism=Caenorhabditis elegans, GI17506829, Length=488, Percent_Identity=41.1885245901639, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71984413, Length=488, Percent_Identity=41.1885245901639, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=41.0309278350515, Blast_Score=313, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=41.0309278350515, Blast_Score=312, Evalue=3e-85,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=40.3292181069959, Blast_Score=314, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6319279, Length=434, Percent_Identity=41.2442396313364, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=40.6952965235174, Blast_Score=325, Evalue=8e-89,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=40.8163265306122, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24648966, Length=411, Percent_Identity=42.0924574209246, Blast_Score=302, Evalue=4e-82,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=34.1666666666667, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24646914, Length=311, Percent_Identity=36.6559485530547, Blast_Score=186, Evalue=5e-47,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 65282; Mature: 65150

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALIRTKIVCTIGPACNSLEKIIELINVGMNVARLNFSHGTQEEHLRTINLLKEARCQLN
CCEEEEEEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEC
LPLAIMLDTKGPEIRLGKIRDGQIFLTVGQKWRLVKKEVLGDESQVSIFPLNILDQLPVG
CCEEEEEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHHHCCCCC
TTILFDDGYIASRVIENSSEGVLVEINNSGMIRSSKGVNIPNTSLNLPAVTEKDIDDIRF
EEEEEECCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHC
GCSQDIDLIAASFVRSAEHVLEIKRLLADEKKTDILVIAKIENSEGVQNFDSIVQAADGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCE
MIARGDLGVEVPLSHVPRLQKMMIRKSYLAGKPVVTATQMLESMINNPRPTRAETSDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIYDSTSAIMLSGETAIGRYPVETVNVMRSIVEEAEADFNYSTFFDQHAPLVYHDVPSAV
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCE
TLATVKTAYSSSAKAIFAFTKAGTTARLLSRLRPKMPIIAMTAKEKIFHQLAFNWGVIPF
EHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCHH
LSEPCNSLDKSLKKISDFALGSQHVSYGDLVVITAGTPFGFSGTTNMMIVENIGDVLVRG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEC
HSGYGIRVHGNVVIVLAPQSKAPYAVKDQVLVIAKCDESYLSLMKEASAIILQNHINDFA
CCCCEEEEECCEEEEEECCCCCCCEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SEKYAKKIALSLGKPILLRADAACQILKEGQLVTLDPEKALVYKGVVLDQLLASGEFS
HHHHHHHHHHHCCCCEEEECHHHHHHHHCCCEEEECCCHHEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALIRTKIVCTIGPACNSLEKIIELINVGMNVARLNFSHGTQEEHLRTINLLKEARCQLN
CEEEEEEEEEECHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEC
LPLAIMLDTKGPEIRLGKIRDGQIFLTVGQKWRLVKKEVLGDESQVSIFPLNILDQLPVG
CCEEEEEECCCCCEEEEEEECCEEEEEECCCHHHHHHHHCCCCCCEEEEEHHHHHCCCCC
TTILFDDGYIASRVIENSSEGVLVEINNSGMIRSSKGVNIPNTSLNLPAVTEKDIDDIRF
EEEEEECCHHHHHHHCCCCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHC
GCSQDIDLIAASFVRSAEHVLEIKRLLADEKKTDILVIAKIENSEGVQNFDSIVQAADGI
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCE
MIARGDLGVEVPLSHVPRLQKMMIRKSYLAGKPVVTATQMLESMINNPRPTRAETSDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHH
AIYDSTSAIMLSGETAIGRYPVETVNVMRSIVEEAEADFNYSTFFDQHAPLVYHDVPSAV
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCE
TLATVKTAYSSSAKAIFAFTKAGTTARLLSRLRPKMPIIAMTAKEKIFHQLAFNWGVIPF
EHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCHH
LSEPCNSLDKSLKKISDFALGSQHVSYGDLVVITAGTPFGFSGTTNMMIVENIGDVLVRG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEEEC
HSGYGIRVHGNVVIVLAPQSKAPYAVKDQVLVIAKCDESYLSLMKEASAIILQNHINDFA
CCCCEEEEECCEEEEEECCCCCCCEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
SEKYAKKIALSLGKPILLRADAACQILKEGQLVTLDPEKALVYKGVVLDQLLASGEFS
HHHHHHHHHHHCCCCEEEECHHHHHHHHCCCEEEECCCHHEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7549104 [H]