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The map label for this gene is pckG [H]

Identifier: 46447005

GI number: 46447005

Start: 1640925

End: 1642700

Strand: Reverse

Name: pckG [H]

Synonym: pc1371

Alternate gene names: 46447005

Gene position: 1642700-1640925 (Counterclockwise)

Preceding gene: 46447006

Following gene: 46447004

Centisome position: 68.04

GC content: 39.75

Gene sequence:

>1776_bases
ATGAAATATGAAGAATTAAAACCTTGGATTCAAGAAATCGCTGAACTTTGTCAACCTCAAATTGTGCATATTTGCGATGG
GTCTAAAAAAGAATACGAAGATTTATGCGCTTTAATGGTTAAAAAAGGAACTTTGATCCCATTAAATGCAGCTAAACGTT
CTCAAAGTTATTTATGCCGCTCAGACCCCCATGATGTGGCTAGAGTTGAAGATAGAACGTTTATTTGTTCTAAAAATTCT
GAAGATGCTGGACCTACTAATCAGTGGCGTGAACCTTTAGAAATGAAACAAACTTTGCGGACCCTTTTTAAAGGTTCAAT
GCAGGGAAGGACGCTATATGTGATTCCATTTAGTATGGGTCCTTTAGGTTCTAAGATTTCTCATTTAGGAGTTCAAATTA
CTGATTCACCCTACGTCGTCTGTAATATGCGATTAATGACGCGCATGGGTAAACAAGTGGAAGAATTATTACCAACTCAA
GAGTTTGTTCCCTGTCTTCATTCCGTCGGAATGCCTTTGAAAGATAGTTTAAAAGATGTCCCGTGGCCTTGTTCTAAAGA
TAAATATATTGTTCATTTTCCTGAAAGTCGTGAAATTTGGTCATTTGGAAGTGGTTATGGAGGAAATGCTCTTTTAGGAA
AAAAATGTTATGCTTTGCGAATTGCTTCTGTGATGGCTCGTGATGAGGGATGGCTAGCAGAGCATATGTTGATCTTAGGT
ATAACAAATCCTCAAGGAGAAAAAAAATATTTAGCGGCTGCCTTTCCCAGTGCTTGTGGAAAAACAAACTTAGCTATGAT
GAAACCCACCTTACCTGGTTGGAAAATTGAAACAGTTGGCGATGATATTGCTTGGATGAAATTTGGGGAAGATGGGCAGT
TATACGCGATTAATCCAGAAGCTGGTTTTTTTGGTGTAGCAAGCGGAACAGCCATGCAATCAAATCCCAACGCCATGTTA
ACAATTGATCGTCAAGTTATTTTTACTAATGTAGCATTGACAGATGAAGGAGATGTGTGGTGGGAAGGGATGACAGAAAC
GCCTCCTCAGCATCTAGTCAATTGGTTAGGGCATGAATGGACACCTCAATCTAAAGAAAAAGCAGCTCATCCTAACTCTC
GTTTTACAGTTGCCGCTAAACAATGTCCGGTTATTGATTCTAAATGGGAAGATCCTAAGGGTGTTCCCATCTCTGCAATT
TTATTTGGCGGACGACGCTCACATACCATCCCTTTAATTTGCGAAGCTTTTAATTGGCAGCACGGAACATTTTTAGGCGC
TTCTATTTCCTCTGAGACGACAGCAGCTTCCACAGGAGAAGTGGGTAAGCTTCGTCATGATCCTTTTGCAATGCTTCCTT
TCTGTGGATACCATATGGGAGATTATTTTAATCACTGGTTGAAAATGGGCCTAAAAAAGGGGGTTAAGCTACCCAAAATT
TTTTACGCAAATTGGTTTAGAAAAGGAGATAAGGGGGATTATCTTTGGCCTGGCTTTGGAGAAAATTGTCGAGTGTTAAA
ATGGATTTTTGAACGCACTTCCGGCAAAGGTCAAGCCATTCGAACACCGATTGGGTATCTGCCAACCGAAGAATCAATTG
ATACGCAAGGGCTTGTTCTCAAACCTCAAGTGATGGAAAAACTTCTTCAAGTAGATCGAGAAGCTTGGCAAAAAGAAATA
CAAGAGATTAGACACTATTTTACCCGTTTTGGGAATAAACTTCCTCAAGCCATTCAGGAAGAATGGCAGCAATTAAAAGA
TCGCTTTAATGGATAA

Upstream 100 bases:

>100_bases
TTTATTAGCAGTTTGTTTAGGAACAGGAAAAGCCCTTGAGTACTTAGATAAATTTAAAAAACGTAAAGCAATTTGAGTTT
TTTAAGAGGAATTTTTCAGC

Downstream 100 bases:

>100_bases
ATGAATTTAAATGATGTCCCTTGATCCTTCTCCTCTTAAAAAAATCAGACGTTTAAGCCAAGCTTTAATGATAAGTGGTG
CATTAAATATTGGAGTCCTC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKYEELKPWIQEIAELCQPQIVHICDGSKKEYEDLCALMVKKGTLIPLNAAKRSQSYLCRSDPHDVARVEDRTFICSKNS
EDAGPTNQWREPLEMKQTLRTLFKGSMQGRTLYVIPFSMGPLGSKISHLGVQITDSPYVVCNMRLMTRMGKQVEELLPTQ
EFVPCLHSVGMPLKDSLKDVPWPCSKDKYIVHFPESREIWSFGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILG
ITNPQGEKKYLAAAFPSACGKTNLAMMKPTLPGWKIETVGDDIAWMKFGEDGQLYAINPEAGFFGVASGTAMQSNPNAML
TIDRQVIFTNVALTDEGDVWWEGMTETPPQHLVNWLGHEWTPQSKEKAAHPNSRFTVAAKQCPVIDSKWEDPKGVPISAI
LFGGRRSHTIPLICEAFNWQHGTFLGASISSETTAASTGEVGKLRHDPFAMLPFCGYHMGDYFNHWLKMGLKKGVKLPKI
FYANWFRKGDKGDYLWPGFGENCRVLKWIFERTSGKGQAIRTPIGYLPTEESIDTQGLVLKPQVMEKLLQVDREAWQKEI
QEIRHYFTRFGNKLPQAIQEEWQQLKDRFNG

Sequences:

>Translated_591_residues
MKYEELKPWIQEIAELCQPQIVHICDGSKKEYEDLCALMVKKGTLIPLNAAKRSQSYLCRSDPHDVARVEDRTFICSKNS
EDAGPTNQWREPLEMKQTLRTLFKGSMQGRTLYVIPFSMGPLGSKISHLGVQITDSPYVVCNMRLMTRMGKQVEELLPTQ
EFVPCLHSVGMPLKDSLKDVPWPCSKDKYIVHFPESREIWSFGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILG
ITNPQGEKKYLAAAFPSACGKTNLAMMKPTLPGWKIETVGDDIAWMKFGEDGQLYAINPEAGFFGVASGTAMQSNPNAML
TIDRQVIFTNVALTDEGDVWWEGMTETPPQHLVNWLGHEWTPQSKEKAAHPNSRFTVAAKQCPVIDSKWEDPKGVPISAI
LFGGRRSHTIPLICEAFNWQHGTFLGASISSETTAASTGEVGKLRHDPFAMLPFCGYHMGDYFNHWLKMGLKKGVKLPKI
FYANWFRKGDKGDYLWPGFGENCRVLKWIFERTSGKGQAIRTPIGYLPTEESIDTQGLVLKPQVMEKLLQVDREAWQKEI
QEIRHYFTRFGNKLPQAIQEEWQQLKDRFNG
>Mature_591_residues
MKYEELKPWIQEIAELCQPQIVHICDGSKKEYEDLCALMVKKGTLIPLNAAKRSQSYLCRSDPHDVARVEDRTFICSKNS
EDAGPTNQWREPLEMKQTLRTLFKGSMQGRTLYVIPFSMGPLGSKISHLGVQITDSPYVVCNMRLMTRMGKQVEELLPTQ
EFVPCLHSVGMPLKDSLKDVPWPCSKDKYIVHFPESREIWSFGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILG
ITNPQGEKKYLAAAFPSACGKTNLAMMKPTLPGWKIETVGDDIAWMKFGEDGQLYAINPEAGFFGVASGTAMQSNPNAML
TIDRQVIFTNVALTDEGDVWWEGMTETPPQHLVNWLGHEWTPQSKEKAAHPNSRFTVAAKQCPVIDSKWEDPKGVPISAI
LFGGRRSHTIPLICEAFNWQHGTFLGASISSETTAASTGEVGKLRHDPFAMLPFCGYHMGDYFNHWLKMGLKKGVKLPKI
FYANWFRKGDKGDYLWPGFGENCRVLKWIFERTSGKGQAIRTPIGYLPTEESIDTQGLVLKPQVMEKLLQVDREAWQKEI
QEIRHYFTRFGNKLPQAIQEEWQQLKDRFNG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI66346721, Length=599, Percent_Identity=51.5859766277129, Blast_Score=636, Evalue=0.0,
Organism=Homo sapiens, GI187281517, Length=601, Percent_Identity=52.0798668885191, Blast_Score=633, Evalue=0.0,
Organism=Homo sapiens, GI66346723, Length=391, Percent_Identity=52.1739130434783, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17555492, Length=614, Percent_Identity=50.3257328990228, Blast_Score=613, Evalue=1e-175,
Organism=Caenorhabditis elegans, GI71989645, Length=600, Percent_Identity=48.3333333333333, Blast_Score=584, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71989658, Length=600, Percent_Identity=48.3333333333333, Blast_Score=583, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI71989653, Length=587, Percent_Identity=48.5519591141397, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI32564411, Length=547, Percent_Identity=52.2851919561243, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI32564407, Length=521, Percent_Identity=52.5911708253359, Blast_Score=547, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI71989649, Length=498, Percent_Identity=50.2008032128514, Blast_Score=508, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI32564409, Length=369, Percent_Identity=50.9485094850949, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17540956, Length=500, Percent_Identity=28.8, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24655085, Length=599, Percent_Identity=50.9181969949916, Blast_Score=626, Evalue=1e-179,
Organism=Drosophila melanogaster, GI24655082, Length=599, Percent_Identity=52.5876460767947, Blast_Score=620, Evalue=1e-178,
Organism=Drosophila melanogaster, GI161077226, Length=566, Percent_Identity=53.1802120141343, Blast_Score=588, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655088, Length=501, Percent_Identity=53.2934131736527, Blast_Score=556, Evalue=1e-158,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 66824; Mature: 66824

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYEELKPWIQEIAELCQPQIVHICDGSKKEYEDLCALMVKKGTLIPLNAAKRSQSYLCR
CCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCHHHCCCCCCCC
SDPHDVARVEDRTFICSKNSEDAGPTNQWREPLEMKQTLRTLFKGSMQGRTLYVIPFSMG
CCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
PLGSKISHLGVQITDSPYVVCNMRLMTRMGKQVEELLPTQEFVPCLHSVGMPLKDSLKDV
CCCCHHHHCCEEECCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCC
PWPCSKDKYIVHFPESREIWSFGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILG
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
ITNPQGEKKYLAAAFPSACGKTNLAMMKPTLPGWKIETVGDDIAWMKFGEDGQLYAINPE
ECCCCCCHHEEHHHCCHHCCCCCEEEECCCCCCCEEEECCCCEEEEEECCCCCEEEECCC
AGFFGVASGTAMQSNPNAMLTIDRQVIFTNVALTDEGDVWWEGMTETPPQHLVNWLGHEW
CCEEEECCCCEECCCCCEEEEEECEEEEEEEEEECCCCEEECCCCCCCHHHHHHHHCCCC
TPQSKEKAAHPNSRFTVAAKQCPVIDSKWEDPKGVPISAILFGGRRSHTIPLICEAFNWQ
CCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCC
HGTFLGASISSETTAASTGEVGKLRHDPFAMLPFCGYHMGDYFNHWLKMGLKKGVKLPKI
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
FYANWFRKGDKGDYLWPGFGENCRVLKWIFERTSGKGQAIRTPIGYLPTEESIDTQGLVL
HHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEE
KPQVMEKLLQVDREAWQKEIQEIRHYFTRFGNKLPQAIQEEWQQLKDRFNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYEELKPWIQEIAELCQPQIVHICDGSKKEYEDLCALMVKKGTLIPLNAAKRSQSYLCR
CCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCHHHCCCCCCCC
SDPHDVARVEDRTFICSKNSEDAGPTNQWREPLEMKQTLRTLFKGSMQGRTLYVIPFSMG
CCCCHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCC
PLGSKISHLGVQITDSPYVVCNMRLMTRMGKQVEELLPTQEFVPCLHSVGMPLKDSLKDV
CCCCHHHHCCEEECCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHCC
PWPCSKDKYIVHFPESREIWSFGSGYGGNALLGKKCYALRIASVMARDEGWLAEHMLILG
CCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEE
ITNPQGEKKYLAAAFPSACGKTNLAMMKPTLPGWKIETVGDDIAWMKFGEDGQLYAINPE
ECCCCCCHHEEHHHCCHHCCCCCEEEECCCCCCCEEEECCCCEEEEEECCCCCEEEECCC
AGFFGVASGTAMQSNPNAMLTIDRQVIFTNVALTDEGDVWWEGMTETPPQHLVNWLGHEW
CCEEEECCCCEECCCCCEEEEEECEEEEEEEEEECCCCEEECCCCCCCHHHHHHHHCCCC
TPQSKEKAAHPNSRFTVAAKQCPVIDSKWEDPKGVPISAILFGGRRSHTIPLICEAFNWQ
CCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCC
HGTFLGASISSETTAASTGEVGKLRHDPFAMLPFCGYHMGDYFNHWLKMGLKKGVKLPKI
CCEEEECCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
FYANWFRKGDKGDYLWPGFGENCRVLKWIFERTSGKGQAIRTPIGYLPTEESIDTQGLVL
HHHHHHCCCCCCCEECCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEE
KPQVMEKLLQVDREAWQKEIQEIRHYFTRFGNKLPQAIQEEWQQLKDRFNG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA