Click here to switch to the map view.

The map label for this gene is proS

Identifier: 46446958

GI number: 46446958

Start: 1580274

End: 1581797

Strand: Reverse

Name: proS

Synonym: pc1324

Alternate gene names: 46446958

Gene position: 1581797-1580274 (Counterclockwise)

Preceding gene: 46446959

Following gene: 46446950

Centisome position: 65.51

GC content: 40.49

Gene sequence:

>1524_bases
ATGACAAGTACAAAAGATAAAACTGCTATTACACCCACAAGAGAAAATGACTATCCAGAATGGTATCAGCAAGTCGTTAA
AGCCTCGGATCTCGCTGAAAATTCACCTGTGCGCGGCAGTATGGTGATCAAACCTTGGGGTTATGGAATATGGGAAAACA
TTCAGCGGCAGTTAGACGATCGGATTAAAGAGACTGGACATGAGAATGCGTATTTTCCTCTTTTCATTCCCTTAAGTTTT
TTAGAAAAAGAAGCGGCGCACATAGAAGGTTTTGCTAAAGAATGTGCCGTTGTGACGCACCATCGCTTAGAAGAAAAGGA
CGGCCGTTTGATCCCTGCAGGTCCTTTAGAAGAGCCATTAATTGTGAGGCCCACCTCGGAGACAATCATTGGGGATTCTT
TTTCTCGTTGGGTAGAATCTTATCGTGATTTACCTCTTTTAATTAATCAATGGGCGAATGTTGTTCGATGGGAGATGCGT
CCACGCATCTTTTTAAGGACGACAGAATTTCTATGGCAAGAAGGGCATACTGCTCATGCAACACATGAAGAAGCCTTACA
AGAAACCATGACAATGTTAGAAGTCTATCGCTCTTTTGTTGAAGATGTCTTAGCTATTCCTGTGATTGTCGGAGAAAAGT
CTCCCGGAGAAAGATTTCCTGGCGCTGAAAATACCTTCACTTTAGAAGCCATGATGCAAGATCGTAAGGCTTTACAATCC
TGTACTTCTCATTATCTAGGGCAAAATTTTGCTAAAGGTTCTAATATTCGCTTTAGTAATATGGAAGGGCAATTAGAATA
TGCTTATACGACATCTTGGGGAATGACAACGCGATTGATTGGAAGTTTAATTATGTGTCATGGAGACGATGATGGATTGA
GACTTCCTCCCCGTATTGCCCATAAACAAATTGTAATTGTTCCAGTCATCCCTAAACCTGAATTGGAAGCTCAAGTTCTC
GAATATGCTGAAAATTTGGCGGCTGAATTGAGAAAACAAATTTTTTACGGCAAACCTTTAACCGTTCATATTGATAAAAG
AGATAAACGAGGCGGAGAGAAAAATTGGGAGTGGGTTAAAAAAGGGGTCCCTCTGCGTTTAGAGGTTGGGCCAAGAGATA
TCAATGAGCAAGCTGTGATGGTAGCAAGAAGAGATCAGTCTCCTAAAAATAAACAATCGTTGCCCAAACAACAATTTGTG
CAGCAAGCTGCTTCAATTTTAGAAGACATTCAACGTAACTATTACGTTCAAGCAGCTACTTACAGAGATCAGCATATTTA
TCGTCATTTAGAAACTTTTGAAGAAATGCGTGCTTTTTTTACTCCTAAGAATGAAGAAAAACCTGAAATTCATGGAGGTT
TTGTTTTAGCAAAGTGGTGTGGAGATCCGGTCACTGAAGAAATGTTAAGCGATTTAAAAGTCACGATTCGTTGCTTACCT
GTGAAGCAAAGTGGAACAAAAGGGCGCTGCATTTTGACAGGACGAGAGGCAACATTAGATGCTATCTTTGCAAAATCGTA
CTGA

Upstream 100 bases:

>100_bases
AAGAAGACGAACATAATTTTAAGCGAGAATTGAATTTGGATGTTAATTTTTTAGAAATATTATAGACTAGCGTTTTTTAT
CAAAAGCTAATGAGAAAACT

Downstream 100 bases:

>100_bases
TCAAAATTTAAGTGGTTGAAAAGGCTGAATTTAATCTACCTCTACTGAATTTGTCTACTTGGCTAAGGTGAGAAAGATGT
TTAATTTTCGCAGCTTATTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MTSTKDKTAITPTRENDYPEWYQQVVKASDLAENSPVRGSMVIKPWGYGIWENIQRQLDDRIKETGHENAYFPLFIPLSF
LEKEAAHIEGFAKECAVVTHHRLEEKDGRLIPAGPLEEPLIVRPTSETIIGDSFSRWVESYRDLPLLINQWANVVRWEMR
PRIFLRTTEFLWQEGHTAHATHEEALQETMTMLEVYRSFVEDVLAIPVIVGEKSPGERFPGAENTFTLEAMMQDRKALQS
CTSHYLGQNFAKGSNIRFSNMEGQLEYAYTTSWGMTTRLIGSLIMCHGDDDGLRLPPRIAHKQIVIVPVIPKPELEAQVL
EYAENLAAELRKQIFYGKPLTVHIDKRDKRGGEKNWEWVKKGVPLRLEVGPRDINEQAVMVARRDQSPKNKQSLPKQQFV
QQAASILEDIQRNYYVQAATYRDQHIYRHLETFEEMRAFFTPKNEEKPEIHGGFVLAKWCGDPVTEEMLSDLKVTIRCLP
VKQSGTKGRCILTGREATLDAIFAKSY

Sequences:

>Translated_507_residues
MTSTKDKTAITPTRENDYPEWYQQVVKASDLAENSPVRGSMVIKPWGYGIWENIQRQLDDRIKETGHENAYFPLFIPLSF
LEKEAAHIEGFAKECAVVTHHRLEEKDGRLIPAGPLEEPLIVRPTSETIIGDSFSRWVESYRDLPLLINQWANVVRWEMR
PRIFLRTTEFLWQEGHTAHATHEEALQETMTMLEVYRSFVEDVLAIPVIVGEKSPGERFPGAENTFTLEAMMQDRKALQS
CTSHYLGQNFAKGSNIRFSNMEGQLEYAYTTSWGMTTRLIGSLIMCHGDDDGLRLPPRIAHKQIVIVPVIPKPELEAQVL
EYAENLAAELRKQIFYGKPLTVHIDKRDKRGGEKNWEWVKKGVPLRLEVGPRDINEQAVMVARRDQSPKNKQSLPKQQFV
QQAASILEDIQRNYYVQAATYRDQHIYRHLETFEEMRAFFTPKNEEKPEIHGGFVLAKWCGDPVTEEMLSDLKVTIRCLP
VKQSGTKGRCILTGREATLDAIFAKSY
>Mature_506_residues
TSTKDKTAITPTRENDYPEWYQQVVKASDLAENSPVRGSMVIKPWGYGIWENIQRQLDDRIKETGHENAYFPLFIPLSFL
EKEAAHIEGFAKECAVVTHHRLEEKDGRLIPAGPLEEPLIVRPTSETIIGDSFSRWVESYRDLPLLINQWANVVRWEMRP
RIFLRTTEFLWQEGHTAHATHEEALQETMTMLEVYRSFVEDVLAIPVIVGEKSPGERFPGAENTFTLEAMMQDRKALQSC
TSHYLGQNFAKGSNIRFSNMEGQLEYAYTTSWGMTTRLIGSLIMCHGDDDGLRLPPRIAHKQIVIVPVIPKPELEAQVLE
YAENLAAELRKQIFYGKPLTVHIDKRDKRGGEKNWEWVKKGVPLRLEVGPRDINEQAVMVARRDQSPKNKQSLPKQQFVQ
QAASILEDIQRNYYVQAATYRDQHIYRHLETFEEMRAFFTPKNEEKPEIHGGFVLAKWCGDPVTEEMLSDLKVTIRCLPV
KQSGTKGRCILTGREATLDAIFAKSY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=520, Percent_Identity=37.5, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71991342, Length=518, Percent_Identity=34.9420849420849, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI71991346, Length=370, Percent_Identity=40.8108108108108, Blast_Score=300, Evalue=9e-82,
Organism=Saccharomyces cerevisiae, GI6321808, Length=530, Percent_Identity=36.6037735849057, Blast_Score=322, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24649468, Length=465, Percent_Identity=38.2795698924731, Blast_Score=308, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24649466, Length=465, Percent_Identity=38.7096774193548, Blast_Score=307, Evalue=1e-83,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PARUW (Q6MBK1)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008323.1
- HSSP:   O26708
- ProteinModelPortal:   Q6MBK1
- SMR:   Q6MBK1
- STRING:   Q6MBK1
- GeneID:   2779500
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1324
- NMPDR:   fig|264201.1.peg.1324
- eggNOG:   COG0442
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- PhylomeDB:   Q6MBK1
- ProtClustDB:   PRK08661
- BioCyc:   CPRO264201:PC1324-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 58248; Mature: 58117

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTKDKTAITPTRENDYPEWYQQVVKASDLAENSPVRGSMVIKPWGYGIWENIQRQLDD
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHH
RIKETGHENAYFPLFIPLSFLEKEAAHIEGFAKECAVVTHHRLEEKDGRLIPAGPLEEPL
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCE
IVRPTSETIIGDSFSRWVESYRDLPLLINQWANVVRWEMRPRIFLRTTEFLWQEGHTAHA
EEECCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCCCCCCC
THEEALQETMTMLEVYRSFVEDVLAIPVIVGEKSPGERFPGAENTFTLEAMMQDRKALQS
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHH
CTSHYLGQNFAKGSNIRFSNMEGQLEYAYTTSWGMTTRLIGSLIMCHGDDDGLRLPPRIA
HHHHHHCCCCCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHEEEEECCCCCCCCCHHHC
HKQIVIVPVIPKPELEAQVLEYAENLAAELRKQIFYGKPLTVHIDKRDKRGGEKNWEWVK
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHH
KGVPLRLEVGPRDINEQAVMVARRDQSPKNKQSLPKQQFVQQAASILEDIQRNYYVQAAT
CCCCEEEEECCCCCCCHHEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHCEEEEEEH
YRDQHIYRHLETFEEMRAFFTPKNEEKPEIHGGFVLAKWCGDPVTEEMLSDLKVTIRCLP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEEEEEEE
VKQSGTKGRCILTGREATLDAIFAKSY
ECCCCCCCEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure 
TSTKDKTAITPTRENDYPEWYQQVVKASDLAENSPVRGSMVIKPWGYGIWENIQRQLDD
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHH
RIKETGHENAYFPLFIPLSFLEKEAAHIEGFAKECAVVTHHRLEEKDGRLIPAGPLEEPL
HHHHCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCE
IVRPTSETIIGDSFSRWVESYRDLPLLINQWANVVRWEMRPRIFLRTTEFLWQEGHTAHA
EEECCCCCHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCCCCCCC
THEEALQETMTMLEVYRSFVEDVLAIPVIVGEKSPGERFPGAENTFTLEAMMQDRKALQS
HHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHH
CTSHYLGQNFAKGSNIRFSNMEGQLEYAYTTSWGMTTRLIGSLIMCHGDDDGLRLPPRIA
HHHHHHCCCCCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHEEEEECCCCCCCCCHHHC
HKQIVIVPVIPKPELEAQVLEYAENLAAELRKQIFYGKPLTVHIDKRDKRGGEKNWEWVK
CCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHH
KGVPLRLEVGPRDINEQAVMVARRDQSPKNKQSLPKQQFVQQAASILEDIQRNYYVQAAT
CCCCEEEEECCCCCCCHHEEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHCEEEEEEH
YRDQHIYRHLETFEEMRAFFTPKNEEKPEIHGGFVLAKWCGDPVTEEMLSDLKVTIRCLP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHEEEEEEEE
VKQSGTKGRCILTGREATLDAIFAKSY
ECCCCCCCEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA