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The map label for this gene is murD

Identifier: 46446885

GI number: 46446885

Start: 1491898

End: 1493232

Strand: Reverse

Name: murD

Synonym: pc1251

Alternate gene names: 46446885

Gene position: 1493232-1491898 (Counterclockwise)

Preceding gene: 46446886

Following gene: 46446884

Centisome position: 61.85

GC content: 37.15

Gene sequence:

>1335_bases
ATGCCGATAGAAAATTACTTTGGGAAAAAAGCGCTCATCATTGGATTAGGGGTAAGTGGAAGAGCCGCGGCTTCTTTCTT
GCAAACATCTGGAGTAAAAGTTTTAGGGGTGGATCGAGATTATCTTCAATTGGAAAATCAACCTGAAATTTTAGAACTGA
AAGCAAAGGGATTGGTAATTCAAGCGGATTGTGAAACTATTGATTTAAAAACATTTGATTTAATCGTCGTTTCTCCTGGA
ATTCCTTCAAATCACCCTTTAATTAAAAAAGCGCACGAACATAAAAAAGAGGTAATCGGAGAAATTGAATTGGGATGCCG
CGCGTCTTGTCATTCGATTATTGGTGTGACAGGAACTAATGGCAAAACAACGGTCACTTTATTAATTACACATATTTTGA
ATGCAAATGGATTAAAAGCTAAAGCTTTAGGTAATGTAGGGACTCCCTTAACAAGAGAAATTTTAACACTCGATTTTCAA
GAAATTGCCGTTTTGGAGCTTAGCTCCTATCAGCTTGACACTTTTCAACAACAGGTTTTGGACGAAGCTGTTGTTTTGAA
TATCACTCCTGATCATTTAGAGCGCTATGAGACAATGGAAAACTATGCAAAATCGAAATTTCAAATTGGAAAATGTTTAA
AAAAATCGGGAAAATTATTTGTCGAGAGGAAAGCTGCCGTCGAATACCAGCATCTTTTAAATTTTCCTCATTCGACGTAT
GGTTATTTACCTTCTTGTCATACTTATACAGATTTATGCTCGGTCTTCTTAGAAGGAATGCAGCAGTTTGAGTTACCGCT
AGAATGGCAAGGGAAAGAAAATCATGATCTAGAAAATCTCTTAGCTGCCTATGCCATTTGCGCACGACGAGGAATTAAAC
CTCAGCAATTTTTACAGGCTTTAAAGACTTTTCAAAAGCCCTCGCATCGTGTCGAGTTTGTTTTAGAAAAGAATGGGATT
CATTTTTATGATGATAGCAAAGGAACGAATATTGATGCGGTTATGCGAGCTGTACAATCGATCAATCGTCCTGTATTTTT
GATCGCTGGAGGAGTGGATAAAGGGGCTTCTTATGTGGCTTGGTTAAATGTTTTTAAAGGTCATGTAAAAAAAGTTTATG
CCATAGGGCAAGCGGCAAATAAAATTCAAAAAGAATTATCACTGCATATTAGAGTGCAAATCGAAGCAAATTTAGATGAC
GCTGTCAAGCAGGCTTATCAGGATGCTAAAGAAGGAGACGCTGTTTTACTCTCTCCCGGTTGTGCAAGTTTTGATATGTT
TAAAGATTATATCCATCGTGGAGAAGAGTTTAAAAGGCTCGTAAAACTTCTATAA

Upstream 100 bases:

>100_bases
GGCCCGAAACTAAAGTTGTGATTCGATTTTGGATCATGAGTTTACTCTTTGCTATCATTGGCATTGCCTCTTTAAAATTT
CAATAAAGGATTGATAACAG

Downstream 100 bases:

>100_bases
ATAAGAGAAATAAAAAAAATAGAGAGGATAAATAAAATGACAAGAAAGGATACGATACTGATTGCTGTCGTTATCAATGC
TGGATTGTTAGCGATTTTAT

Product: putative UDP-N-acetylmuramoylalanine-D-glutamate ligase murD

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MPIENYFGKKALIIGLGVSGRAAASFLQTSGVKVLGVDRDYLQLENQPEILELKAKGLVIQADCETIDLKTFDLIVVSPG
IPSNHPLIKKAHEHKKEVIGEIELGCRASCHSIIGVTGTNGKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQ
EIAVLELSSYQLDTFQQQVLDEAVVLNITPDHLERYETMENYAKSKFQIGKCLKKSGKLFVERKAAVEYQHLLNFPHSTY
GYLPSCHTYTDLCSVFLEGMQQFELPLEWQGKENHDLENLLAAYAICARRGIKPQQFLQALKTFQKPSHRVEFVLEKNGI
HFYDDSKGTNIDAVMRAVQSINRPVFLIAGGVDKGASYVAWLNVFKGHVKKVYAIGQAANKIQKELSLHIRVQIEANLDD
AVKQAYQDAKEGDAVLLSPGCASFDMFKDYIHRGEEFKRLVKLL

Sequences:

>Translated_444_residues
MPIENYFGKKALIIGLGVSGRAAASFLQTSGVKVLGVDRDYLQLENQPEILELKAKGLVIQADCETIDLKTFDLIVVSPG
IPSNHPLIKKAHEHKKEVIGEIELGCRASCHSIIGVTGTNGKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQ
EIAVLELSSYQLDTFQQQVLDEAVVLNITPDHLERYETMENYAKSKFQIGKCLKKSGKLFVERKAAVEYQHLLNFPHSTY
GYLPSCHTYTDLCSVFLEGMQQFELPLEWQGKENHDLENLLAAYAICARRGIKPQQFLQALKTFQKPSHRVEFVLEKNGI
HFYDDSKGTNIDAVMRAVQSINRPVFLIAGGVDKGASYVAWLNVFKGHVKKVYAIGQAANKIQKELSLHIRVQIEANLDD
AVKQAYQDAKEGDAVLLSPGCASFDMFKDYIHRGEEFKRLVKLL
>Mature_443_residues
PIENYFGKKALIIGLGVSGRAAASFLQTSGVKVLGVDRDYLQLENQPEILELKAKGLVIQADCETIDLKTFDLIVVSPGI
PSNHPLIKKAHEHKKEVIGEIELGCRASCHSIIGVTGTNGKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQE
IAVLELSSYQLDTFQQQVLDEAVVLNITPDHLERYETMENYAKSKFQIGKCLKKSGKLFVERKAAVEYQHLLNFPHSTYG
YLPSCHTYTDLCSVFLEGMQQFELPLEWQGKENHDLENLLAAYAICARRGIKPQQFLQALKTFQKPSHRVEFVLEKNGIH
FYDDSKGTNIDAVMRAVQSINRPVFLIAGGVDKGASYVAWLNVFKGHVKKVYAIGQAANKIQKELSLHIRVQIEANLDDA
VKQAYQDAKEGDAVLLSPGCASFDMFKDYIHRGEEFKRLVKLL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=459, Percent_Identity=32.0261437908497, Blast_Score=184, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PARUW (Q6MBS4)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008250.1
- ProteinModelPortal:   Q6MBS4
- STRING:   Q6MBS4
- GeneID:   2780007
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1251
- NMPDR:   fig|264201.1.peg.1251
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   FQVGRHR
- PhylomeDB:   Q6MBS4
- ProtClustDB:   CLSK2762359
- BioCyc:   CPRO264201:PC1251-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49497; Mature: 49366

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIENYFGKKALIIGLGVSGRAAASFLQTSGVKVLGVDRDYLQLENQPEILELKAKGLVI
CCCCHHCCCEEEEEEECCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCEEEEEECCEEE
QADCETIDLKTFDLIVVSPGIPSNHPLIKKAHEHKKEVIGEIELGCRASCHSIIGVTGTN
EECCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQEIAVLELSSYQLDTFQQQVL
CCEEHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHH
DEAVVLNITPDHLERYETMENYAKSKFQIGKCLKKSGKLFVERKAAVEYQHLLNFPHSTY
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCC
GYLPSCHTYTDLCSVFLEGMQQFELPLEWQGKENHDLENLLAAYAICARRGIKPQQFLQA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LKTFQKPSHRVEFVLEKNGIHFYDDSKGTNIDAVMRAVQSINRPVFLIAGGVDKGASYVA
HHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
WLNVFKGHVKKVYAIGQAANKIQKELSLHIRVQIEANLDDAVKQAYQDAKEGDAVLLSPG
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCC
CASFDMFKDYIHRGEEFKRLVKLL
CCHHHHHHHHHHCCHHHHHHHHHC
>Mature Secondary Structure 
PIENYFGKKALIIGLGVSGRAAASFLQTSGVKVLGVDRDYLQLENQPEILELKAKGLVI
CCCHHCCCEEEEEEECCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCEEEEEECCEEE
QADCETIDLKTFDLIVVSPGIPSNHPLIKKAHEHKKEVIGEIELGCRASCHSIIGVTGTN
EECCCEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
GKTTVTLLITHILNANGLKAKALGNVGTPLTREILTLDFQEIAVLELSSYQLDTFQQQVL
CCEEHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHCCHHHHEEEEECCCHHHHHHHHHH
DEAVVLNITPDHLERYETMENYAKSKFQIGKCLKKSGKLFVERKAAVEYQHLLNFPHSTY
CCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCC
GYLPSCHTYTDLCSVFLEGMQQFELPLEWQGKENHDLENLLAAYAICARRGIKPQQFLQA
CCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LKTFQKPSHRVEFVLEKNGIHFYDDSKGTNIDAVMRAVQSINRPVFLIAGGVDKGASYVA
HHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
WLNVFKGHVKKVYAIGQAANKIQKELSLHIRVQIEANLDDAVKQAYQDAKEGDAVLLSPG
HHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCC
CASFDMFKDYIHRGEEFKRLVKLL
CCHHHHHHHHHHCCHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA